163,486 results

1. pCMV-T7 RNA POL

   Plasmid

   #138352

   Purpose

   Expressing T7 RNA Polymerase in mammalian cells

   Depositor

   Baisong Lu

   Insert

   T7 RNA POL
   

   Use

   CRISPR and Lentiviral

   Tags

   No

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV-evoCAST-Cas6

   Plasmid

   #234725

   Purpose

   Engineered human-codon optimized PseCas6 in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   PseCas6
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV-evoCAST-eCas7

   Plasmid

   #234726

   Purpose

   Engineered human-codon optimized PseCas7 in mammalian cells (for evoCAST)

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   ePseCas7
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pU6-hROSA26-1-1kbdonor

   Plasmid

   #234736

   Purpose

   PseCAST crRNA targeting hROSA26-1, with 1 kb donor transposon

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   hROSA26-1 crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. lentiCRISPR v2-Blast

   Plasmid

   #83480

   Purpose

   Mammalian expression of Cas9 and sgRNA scaffold

   Depositor

   Mohan Babu

   Insert

   Blasticidin S deaminase
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Replaced puromycin N-acetyltransferase on the ori…

   Promoter

   EF-1α

   Available Since

   Nov. 10, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRL-SV40P

   Plasmid

   #27163

   Purpose

   Renilla SV40 promoter (no enhancer) reporter gene for normalization of luciferase experiments

   Depositor

   Ron Prywes

   Article

   Chen et al Mol Cell Biol. 1999 Jul . 19(7):4695-702

   Insert

   SV40 promoter
   

   Use

   Luciferase

   Tags

   Renilla Luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. lentiCas9-Blast

   Plasmid

   #52962

   Purpose

   Expresses human codon-optimized S. pyogenes Cas9 protein and blasticidin resistance from EFS promoter. 3rd generation lentiviral backbone.

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:

   Has Service

   Lentiviral Prep

   Inserts

   Cas9
   Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EFS-NS

   Available Since

   May 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pU6-AAVS1-5-1kbdonor

   Plasmid

   #234735

   Purpose

   PseCAST crRNA targeting AAVS1-5, with 1 kb donor transposon

   Depositor

   David Liu

   Article

   Witte et al Science. 2025 May 15;388(6748):eadt5199.

   Insert

   AAVS1-5 crRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-Ef1a-DIO ChETA-EYFP

   Plasmid

   #26968

   Purpose

   AAV expression of humanized ChR2 with E123T/H134R mutations fused to EYFP driven by EF1a promoter for optogenetic activation

   Depositor

   Karl Deisseroth

   Article

   Gunaydin et al Nat Neurosci. 2010 Mar;13(3):387-92. doi

   Has Service

   AAV1, AAV5, and AAV9

   Insert

   hChR2(E123T/H134R)-EYFP
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T, H134R

   Promoter

   EF1alpha

   Available Since

   Jan. 28, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXs-3XHA-EGFP-OMP25

    Plasmid

    #83356

    Purpose

    For tagging mitochondria with HA epitopes (HA-MITO construct)

    Depositor

    David Sabatini

    Article

    Chen et al Cell. 2016 Aug 25;166(5):1324-1337.e11.

    Insert

    3XHA-EGFP-OMP25
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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