We narrowed to 4,591 results for: ado
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Plasmid#53367PurposeContains a truncated Arabidopsis beta-carotene hydroxylase cDNA, the product of which converts beta-carotene primarily to monocyclic beta-cryptoxanthin in E. coli. Use with pAC-BETA or pAC-BETAipi.DepositorInsertchyB1 (BETA-OHASE 1 Mustard Weed)
ExpressionBacterialMutationDeleted codons for the first 129 amino acidsPromoterTrcAvailable SinceApril 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF-pIgH-ESCargo(FTV,Orange)
Plasmid#140161PurposeExpresses a regulatable secretory cargo for mammalian cellsDepositorInsertpiGH-FTVNTT-E2-Orange-FKBP(LV)(C22V)
TagsER signal sequence (MGWSCIILFLVATATGAHS) (N termi…ExpressionMammalianPromoterEF-1aAvailable SinceMarch 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pWPXL-NP1
Plasmid#72654PurposeConstitutive expression of NP1DepositorAvailable SinceJan. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
PX458_TEAD1_1
Plasmid#86356PurposeEncodes gRNA for 3' target of human TEAD1DepositorInsertgRNA against TEAD1 (TEAD1 Human)
UseCRISPRAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-1_pET29b
Plasmid#215433PurposeExpression construct for UMG-SP-1 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-1
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
UMG-SP-2_pET29b
Plasmid#215434PurposeExpression construct for UMG-SP-2 gene, codon optimized for expression in E. coliDepositorInsertUMG-SP-2
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTrcHisA-rat-PTP1B
Plasmid#35987DepositorInsertprotein tyrosine phosphatase 1B (Ptpn1 Rat)
TagsHis and Xpress Epitope TagExpressionBacterialAvailable SinceMarch 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
NylB_pET29b
Plasmid#215414PurposeExpression construct for NylB gene, codon optimized for expression in E. coliDepositorInsertNylB
ExpressionBacterialAvailable SinceMay 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNAtet-phaZ
Plasmid#169874PurposeSingle guide RNA plasmid for Cas9 targeting phaZ in P. putidaDepositorInsertsgRNA towards phaZ in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-CamK2-tTA
Plasmid#210731PurposeAAV vector expressing tetracycline-controlled transactivator (tTA) under CamK2 promoter (for expressing tTA in glutamatergic neurons)DepositorInserttetracycline-controlled transactivator (tTA)
UseAAVPromoterCamK2Available SinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Ptrc-base
Plasmid#169243PurposeSuicide vector for integrating expression cassette in P. putida (IPTG induction)DepositorInsertlacI
ExpressionBacterialPromoterlacIqAvailable SinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX208-NanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
Plasmid#125231PurposeFor generating SPARK2 NanoLuc-βarrestin2-TEVp lentivirusDepositorInsertNanoLuc-15aa linker-βarrestin2-HA-GS linker-TEVp
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Cg
Plasmid#61652PurposeGateway vector for generating cyan fluorescent protein fusions in fungiDepositorInsertenhanced cyan fluorescent protein
ExpressionPlantAvailable SinceMarch 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRN3P_T3_BE3_IVT
Plasmid#177015PurposePlasmid to be used as DNA template for in-vitro RNA transcription of the BE3 base editor (C to T) by T3 RNA polymeraseDepositorInsertBE3
UseCRISPR; Vector for in-vitro transcriptionPromoterT3 promoterAvailable SinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
Tt-NylC_pET28a
Plasmid#215431PurposeExpression construct for Tt-NylC gene, codon optimized for expression in E. coliDepositorInsertTt-NylC
ExpressionBacterialAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pgRNAkan-glpR
Plasmid#169873PurposeSingle guide RNA plasmid for Cas9 targeting glpR in P. putidaDepositorInsertsgRNA towards glpR in Pseudomonas putida
UseCRISPR and Synthetic BiologyExpressionBacterialAvailable SinceJuly 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-002
Plasmid#78689PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLX208-gLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
Plasmid#125233PurposeExpresses "sender" trans-cellular NanoLuc in mammalian cellsDepositorInsertgLuc sp-NanoLuc-15aa linker-ICAM-Nrxn3b-HA
UseLentiviralTagsHAExpressionMammalianPromoterCMVAvailable SinceJuly 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFPL-Yh2
Plasmid#61650PurposeGateway vector for generating yellow fluorescent protein fusions in fungiDepositorInsertenhanced yellow fluorescent protein
ExpressionPlantAvailable SinceJune 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
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BC-A1-006
Plasmid#78693PurposeA weak promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP-Terminator
UseSynthetic BiologyAvailable SinceFeb. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1-PH-GAP1IP4BP
Plasmid#20200DepositorAvailable SinceFeb. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
AAV-tLuc
Plasmid#107552PurposetLuc in AAV backboneDepositorInsertLuciferase
UseAAVAvailable SinceJuly 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_NFIL3
Plasmid#86287PurposeDonor vector for 3' FLAG tag of human NFIL3DepositorAvailable SinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_NFIL3_2
Plasmid#86349PurposeEncodes gRNA for 3' target of human NFIL3DepositorInsertgRNA against NFIL3 (NFIL3 Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_NFIL3_1
Plasmid#86348PurposeEncodes gRNA for 3' target of human NFIL3DepositorInsertgRNA against NFIL3 (NFIL3 Human)
UseCRISPRAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-001
Plasmid#78688PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3 Glt1a
Plasmid#98705Purposemammalian expression of Glt1aDepositorInsertGlt1a (Slc1a2 Rat)
ExpressionMammalianMutationI288V (please see depositor comments below)PromoterCMVAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-flag-UTR
Plasmid#183234PurposeMammalian expression empty backboneDepositorTypeEmpty backboneTagsFLAG tagExpressionMammalianPromoterCMVAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_MIXL1
Plasmid#86280PurposeDonor vector for 3' FLAG tag of human MIXL1DepositorAvailable SinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_TEAD3
Plasmid#86281PurposeDonor vector for 3' FLAG tag of human TEAD3DepositorAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHB4
Plasmid#53462PurposeThis plasmid contains an ADHpr driven chimeric protein with SNC1, N-terminal GFP and C-terminal SUC2. It contains flanking homology to SUC2, allowing optional integration.DepositorInsertyeGFP-Snc1-Suc2
ExpressionYeastPromoterADHAvailable SinceMay 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_NR2F6
Plasmid#86284PurposeDonor vector for 3' FLAG tag of human NR2F6DepositorAvailable SinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFETCh_NCoA2
Plasmid#86283PurposeDonor vector for 3' FLAG tag of human NCoA2DepositorAvailable SinceJan. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-004
Plasmid#78691PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-003
Plasmid#78690PurposeA strong promoter drives the expression of wild type luxR, which regulates expression of LVA-tagged GFP under the control of a weak RBS.DepositorInsertBBa_J23100-BBa_B0034-luxR-Terminator-pLux-BBa_B0033-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
BC-A1-013
Plasmid#78701PurposeA weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of LVA-tagged GFP under the control of a strong RBS.DepositorInsertBBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP(LVA)-Terminator
UseSynthetic BiologyAvailable SinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Glt1b
Plasmid#98706Purposemammalian expression of Glt1bDepositorInsertGlt1b (Slc1a2 Rat)
ExpressionMammalianMutationI348V and N365Y (please see depositor comments se…PromoterCMVAvailable SinceJuly 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
PX458_RXRB_1
Plasmid#104050PurposeEncodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFPDepositorAvailable SinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits only