-
Plasmid#229703PurposeLentiviral expression of iRFP670-alpha-cateninA+ in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseLentiviralTagsiRFP670ExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-YAPS94A
Plasmid#84010Purposeexpresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoterDepositorInsertYAP1 (siRNA insensitive) (YAP1 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationS94APromoterTRE-CMVAvailable sinceOct. 31, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_BIRC2_WT
Plasmid#82188PurposeGateway Donor vector containing BIRC2 , part of the Target Accelerator Plasmid Collection.DepositorInsertBIRC2 (BIRC2 Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCEFL GAL4dbd-KLF4 159-329
Plasmid#140148PurposeGAL4-DNA binding domain fusion protein with amino acids 159 to 329 of human KLF4 (repressor domain)DepositorInsertKLF4 aa 159-329 (KLF4 Human)
UseTagsGAL4dbdExpressionMammalianMutationDeleted amino acids 1 to 158 and 330 to 479PromoterEF1aAvailable sinceApril 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_p.R377H
Plasmid#81566PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationR377HPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
Icam 2 SaCas9 YAP sgRNA3
Plasmid#99736PurposeExpresses SaCas9 and a gRNA targeting mouse YAPDepositorInsertSaCas9 gRNA targeting Yap1 (Yap1 Mouse)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_p.N568K
Plasmid#81612PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationN568KPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLViN-iRFP670-α-cateninΔβH
Plasmid#229706PurposeLentiviral expression of iRFP670-alpha-catenin-delta-beta-H in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseLentiviralTagsiRFP670ExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
PX459-CTNNA1(α-catenin CRISPR KO)
Plasmid#229708PurposeKnockout of a-catenin in mammalian cells using CRISPR-Cas9 technologyDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsExpressionMammalianMutationPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-α-cateninV-
Plasmid#229696PurposeTransient expression of GFP-alpha-cateninV- in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseTagsEGFPExpressionMammalianMutationL344PPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFBOH-MHL.RING1B
Plasmid#224712PurposeExpresses N-terminal hexahistidine-tagged human RING1B (RNF2) in insect cells.DepositorInsertRING1b (RNF2 Human)
UseTags6x histidine tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 18, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLV-shFMR1-mCherry
Plasmid#222963PurposeMake lentivirus to knock down human FMR1 geneDepositorInsertFMR1 shRNA (FMR1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR221-SLC50A1_STOP
Plasmid#161394PurposeGateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.DepositorInsertSLC50A1 (SLC50A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 15, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223_CTCF_p.R377C
Plasmid#81584PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationR377CPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CTCF_p.P552S
Plasmid#81514PurposeGateway Donor vector containing CTCF , part of the Target Accelerator Plasmid Collection.DepositorInsertCTCF (CTCF Human)
UseGateway entry vectorTagsExpressionMutationP552SPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIG-829_HA-GD2-28z_CAR_JUN_Retroviral
Plasmid#207506PurposeThis plasmid can be used to generate retrovirus.DepositorInsertJUN, HA-GD2-28z_CAR (JUN Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(RIT)-FLAG-AVITEV
Plasmid#74053Purposeretroviral expression plasmid for human NFATc2/C (with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human)
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterpMSCV-LTRsAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP
Plasmid#174168PurposeLentiviral vector to constitutively express YAP under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationPromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
EFSp-GFP-YAP (S94A)
Plasmid#174169PurposeLentiviral vector to constitutively express TEAD-binding mutant YAP under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationSerine 94 mutated to AlaninePromoterEFSAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGKp-GFP-YAP
Plasmid#174172PurposeLentiviral vector to constitutively express YAP under control of the human PGK promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationPromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Icam 2 SaCas9 YAP sgRNA2
Plasmid#99735PurposeExpresses SaCas9 and a gRNA targeting mouse YAPDepositorInsertSaCas9 gRNA targeting Yap1 (Yap1 Mouse)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
sgYAP-TAZ-3
Plasmid#229453Purposeknockout of YAP1 and WWTR1 (TAZ)DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceDec. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
Icam 2 SaCas9 YAP sgRNA1
Plasmid#99734PurposeExpresses SaCas9 and a gRNA targeting mouse YAPDepositorInsertSaCas9 gRNA targeting Yap1 (Yap1 Mouse)
UseAAV and CRISPRTagsExpressionMammalianMutationPromoterU6Available sinceDec. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
PGKp-GFP-YAP (S94A)
Plasmid#174173PurposeLentiviral vector to constitutively express TEAD-binding mutant YAP under control of the human PGK promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationSerine 94 mutated to AlaninePromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLViN-iRFP670-α-cateninA+ΔβH
Plasmid#229707PurposeLentiviral expression of iRFP670-alpha-cateninA+delta-beta-H in mammalian cellsDepositorInsertCTNNA1 (alpha-catenin) Human (CTNNA1 Human)
UseLentiviralTagsiRFP670ExpressionMammalianMutationamino acids 670-673, RAIM--> GSGSPromoterCMV promoterAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
sgYAP-TAZ-2
Plasmid#229452Purposeknockout of YAP1 and WWTR1 (TAZ)DepositorUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6;bU6;EFSAvailable sinceJan. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
EFSp-GFP-YAP (5SA)
Plasmid#174170PurposeLentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationFive LATS phosphorylation sites (S61, S109, S127,…PromoterEFSAvailable sinceSept. 28, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
PGKp-GFP-YAP (5SA)
Plasmid#174174PurposeLentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the human PGK promoter.DepositorInsertYAP (YAP1 Human)
UseLentiviralTags3x FLAG tagExpressionMammalianMutationFive LATS phosphorylation sites (S61, S109, S127,…PromoterPGKAvailable sinceAug. 19, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pDONR223-MAGI1
Plasmid#23523DepositorInsertMAGI1 (MAGI1 Human)
UseGateway donor vectorTagsExpressionMutationPromoterAvailable sinceJuly 30, 2010AvailabilityAcademic Institutions and Nonprofits only -
pEMS1369
Plasmid#29170PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle105 (HAP1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1368
Plasmid#29169PurposeExpression of the eGFP reporter gene was not detected in the brain using eGFP. Untested in the eye.DepositorInsertPle104 (HAP1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1086
Plasmid#29020PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle106 (HAP1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1084
Plasmid#29019PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle104 (HAP1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1370
Plasmid#29171PurposeExpression of the eGFP reporter gene was not detected in the brain and eye.DepositorInsertPle106 (HAP1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceJan. 6, 2012AvailabilityAcademic Institutions and Nonprofits only -
pEMS1367
Plasmid#29168PurposeExpression of the reporter gene under the control of the MiniPromoter in this plasmid has not been testedDepositorInsertPle103 (HAP1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-NLSExpressionMutationPromoterAvailable sinceNov. 14, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEMS1083
Plasmid#29018PurposePleiades (Ple) MiniPromoter expression pattern described in Portales-Casamar et al., PNAS, 2010 (PMID: 20807748)DepositorInsertPle103 (HAP1 Human)
UsePleiades promoter project [sic, pleaides plieades]TagsEGFP-Cre-NLSExpressionMutationPromoterAvailable sinceJan. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_JUN_WT
Plasmid#82138PurposeGateway Donor vector containing JUN , part of the Target Accelerator Plasmid Collection.DepositorInsertJUN (JUN Human)
UseGateway entry vectorTagsExpressionMutationWTPromoterNoneAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pIG-755_HA-GD2-28z_CAR_RFP-JUN
Plasmid#207494PurposeThis plasmid can be used to generate HDR templates for non-viral knockin into the TRAC locus of human T cells.DepositorInsertRFP-JUN, HA-GD2-28z_CAR (JUN Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV
Plasmid#74055Purposeretroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 Human, AS 1-686 (N terminus deleted))
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
Dynein-2_complex
Plasmid#132536PurposeFull length human (Hs) IFT dynein (dynein-2) complex for Sf9 expression. Contains His-ZZ-TEV-SNAPf-HC, WDR60, WDR34-Strep, LIC3, TCTEX-1, TCTEX1D2, LC8-1, LC8-2, TCTEX-3, Rbl-1, Rbl-2, LC8-like.DepositorInsertsUseTags8x His, Linker-TEV-linker-TEV, SNAPf, Strep, and …ExpressionInsectMutationCodon optimised for Sf9 expressionPromoterAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICH41308
Plasmid#174574PurposeLevel 0 Acceptor for CDS to be N-taggedDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL01005
Plasmid#174575PurposeLevel 0 Acceptor for CDS to be C-taggedDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL01003
Plasmid#174577PurposeLevel 0 Acceptor for creating C-tag modulesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL01002
Plasmid#174576PurposeLevel 0 Acceptor for creating N-tag modulesDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL30019
Plasmid#174585PurposeLevel 0 N-terminal tag module carrying 6xHIS-3CDepositorInsert6xHIS-3C
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL50026
Plasmid#174590PurposeLevel 0 C-terminal tag module carrying 3C-6xHISDepositorInsert3C-6xHIS
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL30018
Plasmid#174584PurposeLevel 0 N-terminal tag module carrying 6xHIS -SUMO-3CDepositorInsert6xHIS -SUMO-3C
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL30015
Plasmid#174582PurposeLevel 0 N-terminal tag module carrying 6xHIS-MBP-3CDepositorInsert6xHIS-MBP-3C
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL50025
Plasmid#174589PurposeLevel 0 C-terminal tag module carrying 6xHIS uncleavableDepositorInsert6xHIS uncleavable
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pICSL30017
Plasmid#174583PurposeLevel 0 N-terminal tag module carrying 6xHIS uncleavableDepositorInsert6xHIS uncleavable
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 6, 2021AvailabilityAcademic Institutions and Nonprofits only