Skip to main content
Addgene
Showing: 1201 - 1250 of 17682 results

  4. pEUK028

    Plasmid
    #180826
    Purpose
    T7-inducible expression construct to produce SwGdmA (SWIT_RS16490) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    SwGdmA (SWIT_RS16490 Synthetic, Sphingomonas wittichii RW1)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180826-plasmid-map-sequence-id-355555

    Enlarge

  5. pEUK037

    Plasmid
    #180829
    Purpose
    T7-inducible expression construct to produce CnGdmB (CNE_RS35790) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    CnGdmB (CNE_RS35790 Cupriavidus necator N-1)
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180829-plasmid-map-sequence-id-355562

    Enlarge

  6. pEUK066

    Plasmid
    #180830
    Purpose
    T7-inducible expression construct to produce NaGdmA (SARO_RS07455) in E. coli
    Depositor
    Gregg Beckham
    Article
    Bleem et al Chem Catalysis, 2022
    Insert
    NaGdmA
    Use
    Tags
    6X His
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    180830-plasmid-map-sequence-id-355559

    Enlarge

  41. pCASB

    Plasmid
    #190175
    Purpose
    Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence
    Depositor
    Jessica Tyler
    Article
    Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:
    Insert
    sgRNA cloning site
    Use
    Tags
    Expression
    Bacterial and Yeast
    Mutation
    Added 5'-agagacc-3' upstream and 5'…
    Promoter
    Available since
    Oct. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    190175-plasmid-map-sequence-id-372326

    Enlarge

  44. pREDCas9

    Plasmid
    #71541
    Purpose
    Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.
    Depositor
    Tao Chen
    Article
    Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10
    Inserts
    Cas9
    lambda red genes
    plasmid curing system
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pBAD
    Available since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only
    71541-plasmid-map-sequence-id-333602

    Enlarge

  48. pComb3XTT

    Plasmid
    #63891
    Purpose
    Backbone of pComb3X containing TT Fab (light and heavy chains) against tetanus toxin, used as phage display/expression control and PCR template for human antibody constant regions (kappa and CH1)
    Depositor
    Carlos Barbas
    Article
    Andris-Widhopf et al J Immunol Methods. 2000 Aug 28;242(1-2):
    Insert
    TT Fab
    Use
    Phage display
    Tags
    6xHis, HA tag, and geneIII
    Expression
    Bacterial
    Mutation
    Promoter
    lacZ
    Available since
    July 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    63891-plasmid-map-sequence-id-450336

    Enlarge

Showing: 1201 - 1250 of 17682 results