We narrowed to 58,322 results for: {{{s}}}

1. R619-M88-303: CMV51p> MERS-CoV S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166013

   Purpose

   mammalian expression of MERS-CoV soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   MERS-CoV S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CMV-minidCas13X.1-RESCUE-S-SV40pA_U6-BbsI-DR_CMV-mCherry-P2A-Puro-BGHpA

   Plasmid

   #171385

   Purpose

   Expression vector for encoding a human codon-optimized minidCas13X.1-RESCUE-S driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

   Depositor

   Hui Yang

   Article

   Xu et al Nat Methods. 2021 May;18(5):499-506. doi

   Insert

   humanized minidCas13X.1-RESCUE-S
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, U6

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. R619-M87-303: CMV51p> SARS-CoV S-2P-T4f-3C-His8-Strep2x2

   Plasmid

   #166012

   Purpose

   mammalian expression of SARS-CoV soluble spike trimer protein

   Depositor

   Dominic Esposito

   Article

   Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

   Insert

   SARS-CoV S (spike)
   

   Use

   Tags

   3C-His8-Strep2x2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Gal1/10 His6-MBP TEV Ura S. cerevisiae expression vector (12URA-C)

   Plasmid

   #48305

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Na

   Tags

   His6 and MBP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET His6 Sumo TEV expression vector with BioBrick polypromoter restriction sites (14-S)

   Plasmid

   #48313

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   His6 and Sumo

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. R619-M67-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6

   Plasmid

   #166018

   Purpose

   mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD)

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S )
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   Promoter

   CMV51

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Gal1/10 His6-MBP TEV Trp S. cerevisiae expression vector (12TRP-C)

   Plasmid

   #48302

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Na

   Tags

   His6 and MBP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Gal1/10 His6-MBP TEV Ade S. cerevisiae expression vector (12ADE-C)

   Plasmid

   #48299

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Na

   Tags

   His6 and MBP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3.1 Flag YFP hNKCC1 N-terminus T,S mutations P676C I730C (NT95)

   Plasmid

   #50913

   Purpose

   Expresses human NKCC1 with mutated Ser and Thr residues in the N-terminus and mutations at P676C I730C. Contains N-terminal 3xFLAG-YFP tag for expression in mammalian cells

   Depositor

   Biff Forbush

   Article

   Monette et al J Biol Chem. 2014 Jan 22.

   Insert

   human NKCC1 (synthetic) (SLC12A2 Human, Synthetic)
   

   Use

   Tags

   3x Flag and YFP

   Expression

   Mammalian

   Mutation

   S150N S155Q S170Q T177A S183R S193E T203A T205A T…

   Promoter

   CMV

   Available since

   Feb. 6, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. PX458-3xHA-FnCas9

    Plasmid

    #130969

    Purpose

    3xHA tagged Cas9 from F. novicida with T2A-EGFP and cloning backbone for sgRNA

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    FnCas9
    

    Use

    CRISPR

    Tags

    3xHA-NLS and NLS-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-His6-FnCas9GFP

    Plasmid

    #130966

    Purpose

    expression of FnCas9-GFP in bacterial cells

    Depositor

    Debojyoti Chakraborty

    Article

    Acharya et al Proc Natl Acad Sci U S A. 2019 Oct 15;11

    Insert

    HA-NLS-FnCas9
    

    Use

    CRISPR

    Tags

    6xHis-mEGFP-TEV-HA-NLS

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFE-Selo-FI-prk

    Plasmid

    #162698

    Purpose

    Expresses S. elongatus Form IB rubisco and S. elongatus Prk

    Depositor

    David Savage

    Article

    Flamholz et al Elife. 2020 Oct 21;9. pii: 59882. doi: 1

    Insert

    S. elongatus Form IB rubisco and S. elongatus Prk
    

    Use

    Tags

    N terminal 6x His tag on PRK

    Expression

    Bacterial

    Mutation

    Promoter

    PLtet0-1 promoter

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pWR63(YUM1)

    Plasmid

    #203336

    Purpose

    episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    YUM1-targeting sequence
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pWR63

    Plasmid

    #203331

    Purpose

    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDL0_175

    Plasmid

    #211335

    Purpose

    C2_Stop linker

    Depositor

    Dmitri Nusinow

    Article

    Lee et al bioRxiv 2025.06.20.660755

    Insert

    Stop codon
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pDL0_176

    Plasmid

    #210771

    Purpose

    S_Start codon

    Depositor

    Dmitri Nusinow

    Article

    Lee et al bioRxiv 2025.06.20.660755

    Insert

    Start linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDL0_267

    Plasmid

    #210773

    Purpose

    C_Stop codon

    Depositor

    Dmitri Nusinow

    Article

    Lee et al bioRxiv 2025.06.20.660755

    Insert

    Stop linker
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDL0_005

    Plasmid

    #210769

    Purpose

    T_Nopaline synthase terminator

    Depositor

    Dmitri Nusinow

    Article

    Lee et al bioRxiv 2025.06.20.660755

    Insert

    Nopline synthase terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pDL0_159

    Plasmid

    #210770

    Purpose

    S_Setaria viridis R1 coding sequence

    Depositor

    Dmitri Nusinow

    Article

    Lee et al bioRxiv 2025.06.20.660755

    Insert

    SvR1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDL0_217

    Plasmid

    #210772

    Purpose

    S_Setaria viridis C1 coding sequence

    Depositor

    Dmitri Nusinow

    Article

    Lee et al bioRxiv 2025.06.20.660755

    Insert

    SvC1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    June 25, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge