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pBW2601_pCAG-PV1-iCre-N251-L1-ABI-NLS-BGHpA

Plasmid

#108741

Purpose

Expresses N-terminus to aa251 of Cre recombinase fused to ABI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa251

Use

Cre/Lox and Synthetic Biology

Tags

ABI,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2609_pCAG-PV1-PYL-L1-iCre-252C-NLS-BGHpA

Plasmid

#108742

Purpose

Expresses aa252 to C-terminus of Cre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa252 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2164_pCAG-PV1-FlpO-N168-L1-GID1-NLS-BGHpA

Plasmid

#108749

Purpose

Expresses N-terminus to aa168 of Flp recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa168

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2184_pCAG-PV1-GAI-L1-FlpO-169C-NLS-BGHpA

Plasmid

#108750

Purpose

Expresses aa169 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa169 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2173_pCAG-PV1-FlpO-N374-L1-GID1-NLS-BGHpA

Plasmid

#108751

Purpose

Expresses N-terminus to aa374 of Flp recombinase fused to GID1 CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment N-terminus to aa374

Use

Cre/Lox and Synthetic Biology

Tags

GID1,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2193_pCAG-PV1-GAI-L1-FlpO-375C-NLS-BGHpA

Plasmid

#108752

Purpose

Expresses aa375 to C-terminus of Flp recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Flp recombinase fragment aa375 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2230_pCAG-PV1-GAI-L1-iCre-252C-NLS-BGHpA

Plasmid

#108726

Purpose

Expresses aa252 to C-terminus of Cre recombinase fused to GAI CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa252 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

GAI and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2598_pCAG-PV1-iCre-N229-L1-FRB-NLS-BGHpA

Plasmid

#108731

Purpose

Expresses N-terminus to aa229 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa229

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2606_pCAG-PV1-FKBP-L1-iCre-230C-NLS-BGHpA

Plasmid

#108732

Purpose

Expresses aa230 to C-terminus of Cre recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa230 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2600_pCAG-PV1-iCre-N251-L1-FRB-NLS-BGHpA

Plasmid

#108733

Purpose

Expresses N-terminus to aa251 of Cre recombinase fused to FRB CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment N-terminus to aa251

Use

Cre/Lox and Synthetic Biology

Tags

FRB,NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pBW2608_pCAG-PV1-FKBP-L1-iCre-252C-NLS-BGHpA

Plasmid

#108734

Purpose

Expresses aa252 to C-terminus of Cre recombinase fused to FKBP CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa252 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

FKBP and NLS

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pLL5 sgRNA(MS2)-MS2-hA3a D131A

Plasmid

#112131

Purpose

empty sgRNA cloning vector with MS2-humanA3a D131A

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

human Apobec3a D131A mutant (APOBEC3A Human)

Use

CRISPR

Expression

Mammalian

Mutation

D131A mutant in hA3a

Promoter

hU6,CBh

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTol2-10xUAS-Voltron

Plasmid

#119039

Purpose

UAS-driven expresion of Voltron in zebrafish

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Use

Zebrafish expression

Promoter

E1b

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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BACE2_pcDNA6.2/EmGFP-Bsd

Plasmid

#176942

Purpose

Mammalian expression vector encoding BACE2 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

BACE2 (BACE2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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24xMoonTag-kif18b-STOP-24xSunTag-linker-24xPP7 (3’UTR translation reporter)

Plasmid

#128605

Purpose

Expresses 24xgp41 peptide array (MoonTag peptide array), followed by kif18b gene, STOP codon, 24xgcn4 peptide array (SunTag peptide array), linker and 24 repeats of PP7 hairpins.

Depositor

Marvin Tanenbaum

Article

Boersma et al Cell. 2019 Jun 5. pii: S0092-8674(19)304

Inserts

24xMoonTag
kinesin family member 18b (KIF18B Human)
24xSunTag

Expression

Mammalian

Promoter

Pcmv

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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UPRT-mCh-Nluc-P2A-neo-UPRT

Plasmid

#135015

Purpose

Expresses mCherry protein and a fusion protein of Nluc-neo inserting into Cryptosporidium parvum UPRT locus

Depositor

David Sibley

Article

Wilke et al Cell Host Microbe. 2019 Jun 18. pii: S19

Inserts

mCh-Nluc-P2A-neo
UPRT 5'UTR
UPRT 3'UTR

Use

Cryptosporidium expression

Tags

mCherry and Nluc-neo

Promoter

Cryptosporidium actin and enolase

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pX330-sgR26

Plasmid

#127376

Purpose

Used for contransfection with pR26- and pR26-CMVconst-derived constructs to mediate CRISPR/Cas9 genomic insertion into the murine ROSA26 safe harbor locus.

Depositor

Lance Miller

Article

Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

CRISPR and Mouse Targeting

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron

Plasmid

#119033

Purpose

Express Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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PB53x EF1-Dendra2-RPB1Amr

Plasmid

#81228

Purpose

expresses Dendra2-Rpb1 fusion in mammalian cells. Possible to insert into the genome using the Piggibac system

Depositor

Timothée Lionnet

Article

Cho et al Elife. 2016 May 3;5. pii: e13617. doi: 1

Insert

Dendra2 - Rpb1 (alpha amanitin resistant) (POLR2A Human)

Tags

Dendra2

Expression

Mammalian

Mutation

N792D alpha amanitin resistance mutation (Bartolo…

Promoter

EF1a

Available Since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Voltron-ST

Plasmid

#119034

Purpose

Express soma-localized Voltron in mammalian cells

Depositor

Eric Schreiter

Article

Abdelfattah et al Science. 2019 Aug 1. pii: science.aav641

Insert

Voltron-ST

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only

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