We narrowed to 2,324 results for: PUC19

1. pTRERF1-donor

   Plasmid

   #176366

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   TRERF1 (TRERF1 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNR2E3-donor

   Plasmid

   #176369

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   NR2E3 (NR2E3 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pRORB-donor

   Plasmid

   #176354

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   RORB (RORB Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pZBED9-donor

   Plasmid

   #176344

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   ZBED9 (ZBED9 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTSC22D3-donor

   Plasmid

   #176355

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   TSC22D3 (TSC22D3 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pZNF22-donor

   Plasmid

   #176349

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White, Barbara Stranger

   Insert

   ZNF22 (ZNF22 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Expression

   Mutation

   Promoter

   Available since

   Nov. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAW8-aid-3HA-spo5DSR-Turg1:kanMX6

   Plasmid

   #169359

   Purpose

   C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   3XHA

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAW8-aid-V5-spo5DSR-Turg1:kanMX6

   Plasmid

   #169361

   Purpose

   C-terminal aid-V5-spo5DSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   1XV5

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAW8-aid-5Myc-spo5DSR-Turg1:kanMX6

   Plasmid

   #169360

   Purpose

   C-terminal aid-5Myc-spo5DSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Use

   Tags

   5XMyc

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pZNF302-donor

    Plasmid

    #132513

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    ZNF302 (ZNF302 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available since

    July 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pTSHZ1-donor

    Plasmid

    #132479

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Kevin White

    Insert

    TSHZ1 (TSHZ1 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Expression

    Mutation

    none

    Promoter

    Available since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AbVec2.0-mIghg1

    Plasmid

    #127158

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg1 (Ighg1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

    Plasmid

    #219826

    Purpose

    Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 29, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. AbVec2.0-mIghg2c

    Plasmid

    #127159

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg2c (Ighg2c Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hCMV IE1 gene promoter

    Available since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

    Plasmid

    #191390

    Purpose

    Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(1-100)Cy-
    

    Use

    Tags

    mScarlet-I

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLinker-BirA-3xHA-HXGPRT-loxP

    Plasmid

    #86668

    Purpose

    for generation of BirA tagged strains using CRISPR tagging technology

    Depositor

    David Sibley

    Insert

    Linker-BirA-3xHA-HXGPRT 3'UTR
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pUPRT::Floxed DHFR-TS*

    Plasmid

    #100606

    Purpose

    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.

    Depositor

    David Sibley

    Insert

    TgUPRT::Floxed TgDHFR-TS*
    

    Use

    Toxoplasma expression

    Tags

    Expression

    Mutation

    Pyrimethamine resistance

    Promoter

    pTgDHFR-TS

    Available since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pZFX-donor

    Plasmid

    #112366

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZFX with GFP

    Depositor

    Kevin White

    Insert

    ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMS18

    Plasmid

    #225681

    Purpose

    Confers puromycin resistance to Salpingoeca rosetta

    Depositor

    Thibaut Brunet

    Article

    Combredet et al bioRxiv 2024.07.13.603360

    Insert

    pEFL-Pac-Actin_3'UTR
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pDD288

    Plasmid

    #137017

    Purpose

    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    mNG-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized mNG

    Expression

    Worm

    Mutation

    Promoter

    Available since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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