We narrowed to 2,335 results for: PUC19

1. pAW8-aid-3HA-spo5DSR-Turg1:kanMX6

   Plasmid

   #169359

   Purpose

   C-terminal aid-3HA-spo5DSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Tags

   3XHA

   Expression

   Yeast

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAW8-aid-V5-spo5DSR-Turg1:kanMX6

   Plasmid

   #169361

   Purpose

   C-terminal aid-V5-spo5DSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Tags

   1XV5

   Expression

   Yeast

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAW8-aid-5Myc-spo5DSR-Turg1:kanMX6

   Plasmid

   #169360

   Purpose

   C-terminal aid-5Myc-spo5DSR degron cassette with kanMX6 marker for S. pombe

   Depositor

   Tony Carr

   Insert

   auxin-inducible degron (aid)
   

   Tags

   5XMyc

   Expression

   Yeast

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pZNF302-donor

   Plasmid

   #132513

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   ZNF302 (ZNF302 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Mutation

   none

   Available Since

   July 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTSHZ1-donor

   Plasmid

   #132479

   Purpose

   CRISPR donor plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   TSHZ1 (TSHZ1 Human)
   

   Use

   CRISPR

   Tags

   GFP

   Mutation

   none

   Available Since

   March 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV2/2

   Plasmid

   #232199

   Purpose

   AAV packaging plasmid expressing AAV2 Rep and capsid proteins

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Rep2/Cap2
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Sept. 18, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pAAV2/5

   Plasmid

   #232922

   Purpose

   AAV packaging plasmid expressing AAV2 Rep protein and AAV5 capsid protein

   Depositor

   Addgene Research Program, Melina Fan

   Insert

   Rep2/Cap5
   

   Use

   AAV

   Expression

   Mammalian

   Available Since

   Sept. 17, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLinker-BirA-3xHA-HXGPRT-loxP

   Plasmid

   #86668

   Purpose

   for generation of BirA tagged strains using CRISPR tagging technology

   Depositor

   David Sibley

   Insert

   Linker-BirA-3xHA-HXGPRT 3'UTR
   

   Expression

   Bacterial

   Available Since

   May 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. AbVec2.0-mIghg1

   Plasmid

   #127158

   Purpose

   Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG1 isotype

   Depositor

   Hedda Wardemann

   Insert

   Ighg1 (Ighg1 Mouse)
   

   Expression

   Mammalian

   Promoter

   hCMV IE1 gene promoter

   Available Since

   Nov. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. LncRNA overexpression EF1α-BbsI-SV40polyA_puroR

    Plasmid

    #219826

    Purpose

    Overexpression vector for non-coding RNAs driven by EF1α promoter with puromycin resistance. Cloning using BbsI.

    Depositor

    Philipp Maass

    Type

    Empty backbone

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    July 29, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. AbVec2.0-mIghg2c

    Plasmid

    #127159

    Purpose

    Expression of secretory immunoglobulin heavy chains in mammalian cells, mouse (C57BL/6), IgG2c isotype

    Depositor

    Hedda Wardemann

    Insert

    Ighg2c (Ighg2c Mouse)
    

    Expression

    Mammalian

    Promoter

    hCMV IE1 gene promoter

    Available Since

    Nov. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. hROSA26_Hygro_hPGK1_mScarlet-I_hCdt1(1-100)Cy-

    Plasmid

    #191390

    Purpose

    Donor for targeted integration of hCdt1(1-100)Cy- probe to the hROSA26 safe harbor locus

    Depositor

    Yannick Doyon

    Insert

    hCdt1(1-100)Cy-
    

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Feb. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUPRT::Floxed DHFR-TS*

    Plasmid

    #100606

    Purpose

    TgUPRT disruption by knock in with Floxed DHFR-TS*(Pyr-R) cassette with UPRT homology flanks.

    Depositor

    David Sibley

    Insert

    TgUPRT::Floxed TgDHFR-TS*
    

    Use

    Toxoplasma expression

    Mutation

    Pyrimethamine resistance

    Promoter

    pTgDHFR-TS

    Available Since

    Sept. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pZFX-donor

    Plasmid

    #112366

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZFX with GFP

    Depositor

    Kevin White

    Insert

    ZFX homology arms flanking EGFP-IRES-Neo cassette (ZFX Human)
    

    Use

    CRISPR

    Available Since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMS18

    Plasmid

    #225681

    Purpose

    Confers puromycin resistance to Salpingoeca rosetta

    Depositor

    Thibaut Brunet

    Article

    Combredet et al bioRxiv 2024.07.13.603360

    Insert

    pEFL-Pac-Actin_3'UTR
    

    Available Since

    Dec. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDD288

    Plasmid

    #137017

    Purpose

    mNG-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    mNG-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized mNG

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPITX2-donor

    Plasmid

    #176372

    Purpose

    CRISPR donor plasmid to create GFP fusion proteins

    Depositor

    Barbara Stranger, Kevin White

    Insert

    PITX2 (PITX2 Human)
    

    Use

    CRISPR

    Tags

    GFP

    Available Since

    Nov. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD291

    Plasmid

    #137019

    Purpose

    mKate2-GLO^SEC^3xFlag vector with ccdB markers for cloning homology arms

    Depositor

    Daniel Dickinson, Bob Goldstein

    Insert

    mKate2-GLO^SEC^3xFlag + ccdB
    

    Use

    CRISPR and Cre/Lox

    Tags

    3xFlag and C. elegans GLO-optimized mKate2

    Expression

    Worm

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pZBTB5-donor

    Plasmid

    #112303

    Purpose

    CRISPR donor plasmid to tag human transcription factor ZBTB5 with GFP

    Depositor

    Kevin White

    Insert

    ZBTB5 homology arms flanking EGFP-IRES-Neo cassette (ZBTB5 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMT643

    Plasmid

    #8610

    Depositor

    Robert Hartley

    Article

    Hartley et al Biochemistry 1993 Jun 15;32(23):5978-84.

    Insert

    barstar A
    

    Expression

    Bacterial

    Mutation

    Two cysteines of barstar changed to alanine, C40A…

    Available Since

    Jan. 17, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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