We narrowed to 3,323 results for: hwa

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pDONR223-PTK2B

Plasmid

#23898

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PTK2B (PTK2B Human)

Use

Gateway Cloning

Mutation

K42M

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pBabe puro pI3K p110 CAAX

Plasmid

#13339

Depositor

Channing Der

Article

McFall et al Mol Cell Biol. 2001 Aug . 21(16):5488-99

Insert

pI3K p110 CAAX (PIK3CA Bovine)

Use

Retroviral

Expression

Mammalian

Available Since

Oct. 20, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3.1 IGF1R-BirA(R118G)-HA

Plasmid

#109232

Purpose

This construct can be used in the BioID process to identify proteins that interact with the Insulin-like Growth Factor 1 Receptor (IGF1R).

Depositor

Victor Dzau

Article

Bareja et al J Biol Chem. 2018 Mar 12. pii: RA118.002

Insert

IGF1R (IGF1R Human)

Tags

BirA(R118G) (highly promiscuous form) and HA

Expression

Mammalian

Promoter

T7

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBabe-Hygro-GFP

Plasmid

#61215

Purpose

Retrovirus expressing GFP

Depositor

Reuben Shaw

Article

Goodwin et al Mol Cell. 2014 Aug 7;55(3):436-50. doi:

Insert

GFP

Use

Retroviral

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV1

Plasmid

#74981

Purpose

Constitutive ETV1 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV1 (ETV1 Human)

Expression

Mammalian

Promoter

EF1a

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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c-myc promoter (4x TBE2 - wt)

Plasmid

#16599

Depositor

Bert Vogelstein

Article

He et al Science. 1998 Sep 4. 281(5382):1509-12.

Insert

c-myc promoter (4xTBE2 wt) (MYC Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Available Since

March 24, 2008

Availability

Academic Institutions and Nonprofits only
pDONR223-IRAK4

Plasmid

#23749

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

IRAK4 (IRAK4 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pcDNA3-SypHluorin 1x (S1x)

Plasmid

#37003

Depositor

Stephen Heinemann, Yongling Zhu

Article

Zhu et al Neuron. 2009 Feb 12;61(3):397-411.

Insert

SypHluorin (Syp Rat)

Tags

HA

Expression

Mammalian

Mutation

One copy of pHluorin inserted at site 184-185

Promoter

CMV

Available Since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pMK1254

Plasmid

#133057

Purpose

Lentiviral vector with EF1A promoter constitutively expressing a single insert containing the Tau.K18(P301L/V337M)-mRuby2 fusion protein.

Depositor

Martin Kampmann

Article

Chen et al J Biol Chem. 2019 Oct 2. pii: RA119.0094

Insert

Tau.K18(LM)

Use

Lentiviral

Tags

mRuby2

Expression

Mammalian

Promoter

EF1A

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Lis1

Plasmid

#12574

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Lis1 (Pafah1b1 Mouse)

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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beta-catenin (S33Y)-pcw107

Plasmid

#64615

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CTNNB1 (CTNNB1 Human)

Use

Lentiviral

Mutation

S33Y

Promoter

PGK

Available Since

May 12, 2015

Availability

Academic Institutions and Nonprofits only

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pDONR223-BUB1B

Plasmid

#23858

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BUB1B (BUB1B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
HA-FOXO3a TM delta CT

Plasmid

#1797

Depositor

Michael Greenberg

Article

Tran et al Science 2002 Apr 19;296(5567):530-4.

Insert

FOXO3a (FOXO3 Human)

Tags

HA

Expression

Mammalian

Mutation

T32A, S253A, S315A, delta CT (aa 468-673)

Available Since

April 20, 2006

Availability

Academic Institutions and Nonprofits only
pLenti_EF1a_Flag_TP53_WT

Plasmid

#183112

Purpose

Expresses flag-tagged p53 in mammalian cells

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

TP53 (TP53 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

See Depositor Comments Below

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV5

Plasmid

#74984

Purpose

Constitutive ETV5 ORF (codon optimized), blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Inserts

ETV5 (ETV5 Human)
ETV5 (ETV5 Human)

Expression

Mammalian

Mutation

Codon optimized (synonymous)

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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MGAT2-RpHLuorin2

Plasmid

#171718

Purpose

Cis-/medial-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)

Tags

ratiometric pHluorin2

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDONR223-SPHK2

Plasmid

#23714

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

SPHK2 (SPHK2 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pHR_A08_hrGFP

Plasmid

#84615

Purpose

HR donor to integrate UAS1B8TEF(136)-hrGFP-CYC1t into A08 locus

Depositor

Ian Wheeldon

Article

Schwartz et al ACS Synth Biol. 2016 Dec 19.

Insert

hrGFP

Use

Synthetic Biology

Expression

Yeast

Promoter

UAS1B8-TEF(136)

Available Since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pUC CBA-SpCas9.EF1a-BFP.sgLMNA

Plasmid

#98971

Purpose

Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

Depositor

Jan Karlseder

Article

Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

Insert

LMNA sgRNA and spCas9

Expression

Mammalian

Available Since

Dec. 11, 2017

Availability

Academic Institutions and Nonprofits only

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IKKalpha (S176E,S180E)-pcw107-V5

Plasmid

#64608

Purpose

when used to produce lentivirus, express physiological levels of insert

Depositor

David Sabatini, Kris Wood

Article

Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

Insert

CHUK (CHUK Human)

Use

Lentiviral

Tags

V5

Mutation

S176E, S180E

Promoter

PGK

Available Since

June 18, 2015

Availability

Academic Institutions and Nonprofits only

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