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MSCV-P C-FlagHA HPV16-18 E7 Chimera B

Plasmid

#191543

Purpose

Protein expression of HPV16 E7 Chimera B

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

HPV16-18 E7

Use

Retroviral

Tags

FlagHA

Mutation

CR1 CR2 HPV18 E7, C-terminus HPV16 E7

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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MSCV-P C-FlagHA HPV16-18 E7 Chimera C

Plasmid

#191544

Purpose

Protein expression of HPV16 E7 Chimera C

Depositor

Elizabeth White

Article

Nouel et al mBio. 2022 Nov 8:e0203322. doi: 10.1128/

Insert

HPV16-18 E7

Use

Retroviral

Tags

FlagHA

Mutation

CR1 HPV18 E7, CR2 C-terminus HPV16 E7

Available Since

Nov. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pJEP209-Lenti-1.3CaMKII P-Intron-Flag-GluN2A-WPRE-pA

Plasmid

#74714

Purpose

pLenti vector backbone designed to express Flag-GluN2A from a 1.3CaMKII promoter. Transgene in reverse orientation.

Depositor

Jonathan Ploski

Article

Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

Insert

Flag-GluN2A

Use

Lentiviral

Tags

Flag

Promoter

1.3CaMKIIa

Available Since

July 7, 2022

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (G20V)

Plasmid

#119308

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (G20V) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (G20V) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

G20V

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (C21Y)

Plasmid

#119304

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (C21Y) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (C21Y) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

C21Y

Promoter

CMV

Available Since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

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PHAGE-P CMVt N-HA hE6-AP I (R39H)

Plasmid

#119309

Purpose

lentiviral vector for expression of HA-Tagged E6-AP I (R39H) in mammalian cells

Depositor

Peter Howley

Article

Kuhnle et al J Biol Chem. 2018 Sep 26. pii: RA118.004

Insert

E6-AP I (R39H) (UBE3A Human)

Use

Lentiviral

Tags

HA

Mutation

G20V

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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p6330 pHAGE-P-CMVt-N-HA-GAW-HERC2-F6

Plasmid

#100140

Purpose

Encodes amino acids 4421-4834 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 4421-4834

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6329 pHAGE-P-CMVt-N-HA-GAW-HERC2-F5

Plasmid

#100139

Purpose

Encodes amino acids 3550-4500 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 3550-4500

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6328 pHAGE-P-CMVt-N-HA-GAW-HERC2-F4

Plasmid

#100138

Purpose

Encodes amino acids 2600-3600 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 2600-3600

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6326 pHAGE-P-CMVt-N-HA-GAW-HERC2-F2

Plasmid

#100136

Purpose

Encodes amino acids 950-1750 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 950-1750

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p6325 pHAGE-P-CMVt-N-HA-GAW-HERC2-F1

Plasmid

#100135

Purpose

Encodes amino acids 1-1000 of HERC2.

Depositor

Peter Howley

Article

Galligan et al J Proteome Res. 2015 Feb 6;14(2):953-66.

Insert

HERC2 (HERC2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

amino acids 1-1000

Promoter

CMV

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycLAM

Plasmid

#71807

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-LAM

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]MycPC

Plasmid

#71808

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc-PC

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pZ P (cHS4)X4 TetON-3XFLAG-tdT CAGG-m2rtTA v2

Plasmid

#112669

Purpose

Donor vector for FLPe recombinase-mediated cassette exchange in master cell lines created with plasmid #112666. This vector allows inducible expression and contains cHS4 insulators.

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

tdT

Use

AAV; Donor plasmid for recombinase-mediated casse…

Expression

Mammalian

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[open]

Plasmid

#71805

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[open]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pLINK cyclin T1 (1-280) myc (all 200) (P#575)

Plasmid

#14610

Depositor

Matija Peterlin

Article

Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

Insert

cyclin T1 (1-280) (all 200) (CCNT1 Human)

Tags

myc

Expression

Mammalian

Mutation

C198A, C200A, H202A, C205A. Active in 3T3 cells.

Available Since

June 30, 2008

Availability

Academic Institutions and Nonprofits only

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p-attB-FL.hsp70P-Dam[4-HT-intein@L127C]Myc[closed]

Plasmid

#71806

Purpose

Generating transgenic flies for 4-hydroxytamoxifen-inducible DamID

Depositor

Bas van Steensel

Article

Pindyurin et al Nucleic Acids Res. 2016 Jul 8;44(12):564

Insert

FL.hsp70P-Dam[4-HT-intein@L127C]-Myc[closed]

Expression

Insect

Available Since

March 2, 2016

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) Triple RBD

Plasmid

#212412

Purpose

Encodes SARS-CoV-2 spike K417T,E484K,N501Y for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike K417T,E484K,N501Y (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

K417T,E484K,N501Y

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 (P.1) - No RBD Mutations

Plasmid

#212384

Purpose

Encodes SARS-CoV-2 spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F for pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V1176F (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Mutation

L18F,R21N,P26S,D138Y,R190S,D614G,H655Y,T1027I,V11…

Promoter

CAG

Available Since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A_S

Plasmid

#147515

Purpose

Insect Expression of CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A

Depositor

Elisa Izaurralde

Article

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

CeAIN2_254-400-DmGW182_635-1384-CIM1-P-GLmut-F961A (gw Human, Nematode)

Expression

Insect

Mutation

Deletion of 2 AA (SD) at position 378-379 compare…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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