We narrowed to 10,665 results for: t7

1. pT7C-HSPA1L

   Plasmid

   #177660

   Purpose

   Recombinant protein synthesis of human HSPA1L

   Depositor

   Jeff Kuret

   Article

   Ayoub et al PLoS Genet. 2023 Mar 27;19(3):e1010681.

   Insert

   HSPA1L (HSPA1L Human)
   

   Tags

   6xHis-Thrombin

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFS_0271_pET30_pCat-tetR-Term-ptetA-sfGFP-rrnBterm_Fusicatenibacter_saccharivorans_ARRAY2_FaqI

   Plasmid

   #116955

   Purpose

   Expresses FsRT-Cas1-Cas2 under pT7lac and sfGFP under ptetA promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readout

   Depositor

   Randall Platt

   Article

   Schmidt et al Nature. 2018 Oct;562(7727):380-385. doi:

   Insert

   Fusicatenibacter saccharivorans RT-Cas1-Cas2
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET42a-lambdaN+-L+-GSH

   Plasmid

   #98894

   Purpose

   Expresses lambdaN+-L+-GST fusion protein for affinity purification with ARiBo method

   Depositor

   Pascale Legault

   Article

   Di Tomasso et al Nucleic Acids Res. 2011 Feb;39(3):e18. d

   Insert

   lambdaN+-L+
   

   Tags

   GST tag

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   Aug. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRSETB-ro2GFP

   Plasmid

   #82366

   Purpose

   Inducible expression of redox sensitive GFP in bacteria

   Depositor

   S. James Remington

   Article

   Hanson et al J Biol Chem. 2004 Mar 26;279(13):13044-5

   Insert

   ro2GFP
   

   Tags

   6xHIS

   Expression

   Bacterial

   Mutation

   C48S/S65T/Q80R/S147C/Q204C

   Promoter

   T7

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET22b-GFP-CPDSalI

   Plasmid

   #38257

   Depositor

   Matthew Bogyo, Aimee Shen

   Article

   Shen et al PLoS One. 2009 Dec 2;4(12):e8119.

   Insert

   eGFP
   

   Tags

   6xHis and Vibrio cholerae MARTX toxin cysteine pr…

   Expression

   Bacterial

   Mutation

   cloned from pEGFPN3 (Clonetech)

   Promoter

   T7

   Available Since

   July 20, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pET151-Vps4

   Plasmid

   #87733

   Purpose

   Bacterial expression of full-length S. cerevisiae Vps4

   Depositor

   Chris Hill

   Article

   Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

   Insert

   Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
   

   Tags

   6xHis-V5-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDEST14-SpyCatcher002 EQ

   Plasmid

   #102830

   Purpose

   Bacterial expression of unreactive version of SpyCatcher002

   Depositor

   Mark Howarth

   Article

   Keeble et al Angew Chem Int Ed Engl. 2017 Oct 10. doi

   Insert

   SpyCatcher002 EQ
   

   Tags

   GSSGS, His6, and TEV cleavage site

   Expression

   Bacterial

   Mutation

   E77Q mutation prevents isopeptide bond formation

   Promoter

   T7

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBx1-VHH template1-3XMyc-Spacer

   Plasmid

   #176208

   Purpose

   PCR template for generating CeVICA VHH/nanobody library

   Depositor

   Aviv Regev

   Article

   Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

   Insert

   VHH template1-3XMyc-Spacer
   

   Tags

   Myc tag

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDM_noro_A2_lacZA

   Plasmid

   #118819

   Purpose

   T7 RNAP-driven expression of norovirus antisense orientation toehold switches (A2) with a lacZ alpha subunit reporter.

   Depositor

   Alexander Green

   Article

   Ma et al Synth Biol (Oxf). 2018;3(1):ysy018. doi:

   Insert

   Norovirus toehold switch A2
   

   Expression

   Bacterial

   Available Since

   Nov. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTH1-SpSrtA

    Plasmid

    #64987

    Purpose

    Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

    Insert

    S. pyogenes sortase A
    

    Tags

    His6 tag, Mxe GyrA intein, and chitin-binding pro…

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-81

    Promoter

    T7 promoter

    Available Since

    June 5, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCellFree_G03 WWP1

    Plasmid

    #67125

    Purpose

    Standard for cell-free expression benchmarking.

    Depositor

    Kirill Alexandrov

    Article

    Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

    Insert

    WWP1 (WWP1 Human)
    

    Use

    Cell-free expression

    Tags

    EGFP and His

    Promoter

    T7

    Available Since

    Nov. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRSF-1b-S14

    Plasmid

    #128596

    Purpose

    Expresses tagless 30S ribosomal subunit protein S14 in E.coli

    Depositor

    James Williamson

    Article

    Duss et al Cell. 2019 Nov 27;179(6):1357-1369.e16.

    Insert

    30S ribosomal subunit protein S14 (rpsN E.coli)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pETx-SR6c3

    Plasmid

    #176211

    Purpose

    Bacterial expression of the SR6c3 nanobody targeting the receptor binding domain of SARS-CoV-2 spike protein

    Depositor

    Aviv Regev

    Article

    Chen et al Nat Commun. 2021 Sep 17;12(1):5506. doi:

    Insert

    SR6c3
    

    Tags

    6XHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET22b 6His SpyCatcher-GRFT

    Plasmid

    #204502

    Purpose

    Bacterial expression of GRFT with N-terminal SpyCatcher fusion and GS linker

    Depositor

    Urartu Seker

    Article

    Ozkul et al Adv Mater Interfaces. 2022 Sep 13:220112

    Insert

    GRFT
    

    Use

    Synthetic Biology

    Tags

    6xHis and SpyCatcher

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-StrepII-TagRFP-CohI

    Plasmid

    #58710

    Purpose

    Detection construct to label dockerin type I containing fusion proteins

    Depositor

    Hermann Gaub

    Article

    Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

    Insert

    TagRFP-CohI
    

    Tags

    StrepII

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMJ839

    Plasmid

    #39317

    Depositor

    Jennifer Doudna

    Article

    Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

    Insert

    Cas9 (NMA0631 Neisseria meningitidis)
    

    Use

    CRISPR

    Tags

    His6 and MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

17. pMJ915v2 + sfGFP

    Plasmid

    #88918

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-51b(+) ChemoG5

    Plasmid

    #193805

    Purpose

    Protein production of ChemoG5 in bacteria

    Depositor

    Julien Hiblot

    Article

    Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

    Insert

    ChemoG5
    

    Tags

    10xHis

    Expression

    Bacterial

    Mutation

    EGFP:A206K, T225R; HT7: E143R, E147R, L271E

    Promoter

    T7

    Available Since

    June 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJB01

    Plasmid

    #111957

    Purpose

    Expresses His10-RFP-Halo; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Insert

    RFP
    

    Tags

    HaloTag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPTD-DRBD

    Plasmid

    #35622

    Depositor

    Steven Dowdy

    Article

    Eguchi et al Nat Biotechnol. 2009 Jun;27(6):567-71. E

    Insert

    PKR DRBD-1
    

    Tags

    6xHIS, HA, and TAT

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 15, 2012

    Availability

    Academic Institutions and Nonprofits only