We narrowed to 6,994 results for: ef1a

1. TFORF1570

   Plasmid

   #144105

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF680 (ZNF680 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. dCas9-ddMSK1

   Plasmid

   #165603

   Purpose

   Expresses Sp dCas9 fused to truncated inactive human MSK1 (42-802, D195A, D565A)

   Depositor

   Isaac Hilton

   Article

   Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

   Insert

   S. Pyogenes dCas9 with c-terminal truncated inactive human Mitogen- and stress-activated protein kinase-1 (42-802, D195A, D565A) (RPS6KA5 S. Pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. TFORF2895

   Plasmid

   #144355

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   MTA1 (MTA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Flag‐hTim1

   Plasmid

   #49207

   Purpose

   expresses Flag-tagged human Tim1

   Depositor

   Lawrence Kane

   Article

   de Souza et al Proc Natl Acad Sci U S A. 2005 Nov 22;10

   Insert

   Tim1 (HAVCR1 Human)
   

   Use

   Tags

   FLAG and signal sequence

   Expression

   Mammalian

   Mutation

   Tim1 lacks its own signal sequence

   Promoter

   EF1a

   Available since

   Dec. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

5. TFORF0912

   Plasmid

   #143449

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ZNF831 (ZNF831 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRDB_182

   Plasmid

   #216103

   Purpose

   CRISPRi, EF1a-driven dCas9-KRAB (ZIM3) (Cas only)

   Depositor

   John Doench

   Article

   McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

   Insert

   Cas9 [Sp]
   

   Use

   CRISPR and Lentiviral; Assembled vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. EFS-SpdCas9

   Plasmid

   #210703

   Purpose

   This plasmid express EFS promoter driven human codon optimized SpdCas9

   Depositor

   Isaac Hilton

   Article

   Mahata et al Nat Methods. 2023 Oct 9. doi: 10.1038/s4

   Insert

   S. Pyogenes dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   D10A/H840A

   Promoter

   EF1a core promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF.GFP

   Plasmid

   #17616

   Depositor

   Linzhao Cheng

   Article

   Cui et al Blood. 2002 Jan 15. 99(2):399-408.

   Insert

   EF-1a promoter (EEF1A1 Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. TFORF0683

   Plasmid

   #142675

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PRDM12 (PRDM12 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   June 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRDB_181

    Plasmid

    #216102

    Purpose

    CRISPRi, EF1a-driven dCas9-KRAB (ZNF10) (Cas only)

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Insert

    Cas9 [Sp]
    

    Use

    CRISPR and Lentiviral; Assembled vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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