We narrowed to 15,879 results for: NOL
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Plasmid#216037PurposeFragmid fragment: (C' terminus) Nucleoplasmin NLSDepositorHas ServiceCloning Grade DNAInsertNpNLS_v1.1; 3xHA_v1.1
UseFragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
GST-MAGE-2-429
Plasmid#89129Purposebacterial expression of full-length human MAGE-A11 with N-terminal GST tag for rapid isolation, in pGEX-4T-1 EcoRI and XhoI sitesDepositorAvailable SinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOCC292
Plasmid#118881Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with C-terminal mRuby3, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-NotI-ccdB-AscI-mRuby3-3C-HIS6-stop-HindIII cassette
TagsmRuby3, HIS6, cleavable with 3C proteaseExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEL033
Plasmid#137902PurposeSTU2.0 rAPOBEC1-SpyCas9(D10A) for plant genome base editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOCC28
Plasmid#118873Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal MBP tag, cleavable with 3C, and C-terminal HIS6 tag, cleavable with 3CDepositorInsertNcoI-MBP-3C--NotI-ccdB-AscI-3C-HIS6-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease and MBP, cleavab…ExpressionInsectPromoterpolHAvailable SinceJan. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSTAP-seq_human-4xUAS
Plasmid#125150PurposeVector to measure the activity of CP candidates in response to a tethered (4xUAS) GAL4-DBD-COF by determining the abundance of transcripts originating from each candidate in human cellsDepositorInsert4 x upstream activating sequence (UAS)
UseStap-seq screening vectorAvailable SinceAug. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK_P-GAP-ACC1*
Plasmid#126740Purposeyeast plasmid overexpressing ACC1 from Komagataella phaffiiDepositorInsertacc1*
ExpressionYeastMutationT3452GPromoterGAPAvailable SinceOct. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
EK0423 SFFV-mTagBFP2-SGc(s70587)cSG-mCherry (FLP-IN)
Plasmid#191162PurposeExpression of a sensor for synthetic sequence 1 (EK0208) in mammalian cells; mTagBFP2 marker, mCherry outputDepositorInsertmTagBFP:s70587:mCherry
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCaRNA3-eIFiso4E120(+)
Plasmid#86092PurposeThis vector is used to specifically silence the eIF(iso)4E gene in peach via VIGS.DepositorInserteIFiso4E
ExpressionPlantAvailable SinceJan. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pPSP6+t
Plasmid#48149PurposeShuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6DepositorTypeEmpty backboneUseSynthetic BiologyExpressionBacterialAvailable SinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pOCC291
Plasmid#118867Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6 tag, cleavable with 3C, and N-terminal mRuby3DepositorInsertNcoI-HIS6-3C-mRuby3-NotI-ccdB-AscI-stop-HindIII cassette
TagsHIS6, cleavable with 3C protease, mRuby3ExpressionInsectPromoterpolHAvailable SinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAGK112
Plasmid#228127PurposeExpress node 1531 retron with split retron ncRNA and gRNA to edit (SNIP + PAM recode) HEK4 gene in human cellsDepositorInsertRetron node 1531 split retron ncRNA and gRNA to edit HEK4 gene (SNP + PAM recode) in human cells
ExpressionMammalianPromoterCAG for RT, U6/H1 for ncRNA/gRNAAvailable SinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEL029
Plasmid#137898PurposeSTU2.0 SpyCas9 RZ system for plant genome editingDepositorTypeEmpty backboneUseCRISPRExpressionPlantAvailable SinceFeb. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 TGFB1
Plasmid#67080PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGWB529
Plasmid#74871PurposeGateway cloning compatible binary vector for C-terminal fusion with TAP (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceOct. 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB524
Plasmid#74866PurposeGateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).DepositorTypeEmpty backboneExpressionPlantPromoterCaMV35SAvailable SinceNov. 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGWB443
Plasmid#74829PurposeGateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).DepositorTypeEmpty backboneExpressionPlantAvailable SinceSept. 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCfB3035(X-4 MarkerFree)
Plasmid#73273PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-4 (Chr X: 236336..237310)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionYeastAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX SHIP2-SH2
Plasmid#46493DepositorInsertSHIP2
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 8-131PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Syk(NC)-SH2
Plasmid#46521DepositorInsertSyk(NC) (SYK Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 7-263PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
EK0325 SFFV-mCherry-SG(EGFP.s2)SG-Cre (FLP-IN)
Plasmid#191160PurposeExpression of a sensor for EGFP CDS in mammalian cells; mCherry marker, Cre outputDepositorInsertmCherry:EGFP.s2:Cre
ExpressionMammalianMutationWTPromoterSFFVAvailable SinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
p1977 (pUC19-PTDH3-PTEF1)
Plasmid#78230PurposeExample plasmid containing a bidirectional promoter of pTDH3 and pTEF1DepositorInsertBidirectional promoters pTDH3-pTEF1
ExpressionBacterialAvailable SinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLmoCascade-IL1RNcrRNA
Plasmid#126487PurposeEncodes L.monocytogenes CRISPR Type I-B crRNA targeting IL1RNDepositorInsertLmo IL1RN cr03
UseCRISPRExpressionMammalianPromoterU6Available SinceSept. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pssAAV_Seq17_CMV_bglobin_3NLS_Venus_WPRE_polyA
Plasmid#220224PurposeAAV-STARR-seq validation control vector with VenusDepositorTypeEmpty backboneUseAAVExpressionMammalianPromoterCMVAvailable SinceJuly 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSI-535
Plasmid#131130PurposegRNA expression vector for C-to-T base editing reporterDepositorInsertC-to-T reporter activation gRNA
UseCRISPRExpressionMammalianPromoterHuman U6 promoterAvailable SinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CALR
Plasmid#67086PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
piGECI-NES-N1
Plasmid#160422PurposeExpression of a near-infrared genetically encoded calcium indicator iGECI with a nuclear export signal sequenceDepositorInsertiGECI-NES
ExpressionMammalianPromoterCMVAvailable SinceNov. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pZ_lacO-cP-AOX1-NpgA
Plasmid#126731Purposeyeast plasmid overexpressing NpgADepositorInsertnpgA
Tags6xHisExpressionYeastMutationG951A,C957AAvailable SinceJune 28, 2019AvailabilityAcademic Institutions and Nonprofits only