We narrowed to 9,203 results for: sacs

1. pSV CDK9 (D167N) HA (P#601)

   Plasmid

   #14639

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

   Insert

   cdk9 D167N (CDK9 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   D167N. Kinase negative mutant.

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pGEX-2TK cyclin T1 (1-250) (P#467)

   Plasmid

   #14606

   Depositor

   Matija Peterlin

   Article

   Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

   Insert

   cyclin T1 (1-250) (CCNT1 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   amino acids 1-250

   Promoter

   Available since

   June 30, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDN-G1Tt

   Plasmid

   #44509

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al Proc Natl Acad Sci U S A. 2009 Mar 31;10

   Inserts

   PGal1-D12 promoter (GAL1 Synthetic, Budding Yeast)
   tetR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Two tandem tet operators (2XtetO2) downstream of …

   Promoter

   PGal1-D12 promoter

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pCLASP_Crz1(19A)_CLASP_RGS2membrane

   Plasmid

   #133085

   Purpose

   Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   Crz1*-CLASP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   pADH1

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDN-T2dGZmxh

   Plasmid

   #44552

   Depositor

   Gabor Balazsi

   Article

   Nevozhay et al PLoS Comput Biol. 2012;8(4):e1002480. do

   Inserts

   PTETREG promoter
   yEGFP::ZeoR
   

   Use

   Synthetic Biology; Expression regulator/reporter

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   PTETREG

   Available since

   April 23, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. p3xFLAG-MKL1 D301-380

   Plasmid

   #19852

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 301 to 380

   Promoter

   Available since

   Dec. 16, 2008

   Availability

   Academic Institutions and Nonprofits only

7. pEasyG2_zeo

   Plasmid

   #184913

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pEasyG2_nat

   Plasmid

   #184914

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_nat recombines in vivo with a PCR product from pEasyG2_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pEasyG3_zeo

   Plasmid

   #184917

   Purpose

   Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

   Depositor

   Jeferson Gross

   Article

   Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

   Insert

   gRNA scaffold
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Venus-Venus-FLARE-AKAR

    Plasmid

    #123331

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-Venus-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pST1374-GCN4-H840A

    Plasmid

    #113024

    Purpose

    Expresses GCN4-H840A in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    GCN4-H840A (GCN4 S. pyogenes, Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D839A, H840A, N863A

    Promoter

    CMV

    Available since

    July 19, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Cerulean3-Cerulean3-FLARE-EKAR-EV

    Plasmid

    #123342

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AB.pCCL.sin.cPPT.U6.miR-10a-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46581

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-10a Decoy (MIR10A, Mir10a Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pFS_1112_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23109-Leader-ARRAY2-DR2-FaqI_Term_NotI

    Plasmid

    #184740

    Purpose

    encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23109

    Depositor

    Randall Platt

    Article

    Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

    Insert

    FsRT-Cas1(opt)-Cas2(opt)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    sequence codon optimized for expression in E. coli

    Promoter

    pTetA

    Available since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLINK CDK9 myc (D167N) (P#1084)

    Plasmid

    #14637

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 D167N (CDK9 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    D167N. Kinase negative mutant.

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

16. pCin4-3xFLAG-MKL1 D444-500

    Plasmid

    #19839

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 D444-500 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 444-500

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

17. pCin4-3xFLAG-MKL1 T450A

    Plasmid

    #19843

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 T450A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Threonine 450 to Alanine

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

18. AB.pCCL.sin.cPPT.U6.miR-375-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46617

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-375 Decoy (MIR375, Mir375 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pST1374-H840A-GCN4

    Plasmid

    #113027

    Purpose

    Expresses H840A-GCN4 in mammalian cells

    Depositor

    Xingxu Huang

    Article

    Jiang et al Cell Res. 2018 Jun 6. pii: 10.1038/s4142

    Insert

    H840A-GCN4 (GCN4 S. pyogenes, Budding Yeast)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D839A, H840A, N863A

    Promoter

    CMV

    Available since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGEX-2TK cyclin T1 (1-551) (P#422)

    Plasmid

    #14609

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al Proc Natl Acad Sci U S A. 1999 Feb 16. 9

    Insert

    cyclin T1 (1-551) (CCNT1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    amino acids 1-551

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

21. pET28-p53 (1-73) (72R)

    Plasmid

    #62082

    Purpose

    Expression of human p53 (1-73) (72R) in e. coli

    Depositor

    Gary Daughdrill

    Article

    Vise et al Nucleic Acids Res. 2005 Apr 11;33(7):206

    Insert

    p53 (TP53 Human)
    

    Use

    Tags

    HIs

    Expression

    Bacterial

    Mutation

    contains TAD residues 1-73; 72R

    Promoter

    T7

    Available since

    Feb. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pCin4-3xFLAG-MKL1 D500-630

    Plasmid

    #19841

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 D500-630 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 500-630

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

23. p3xFLAG-MKL1 D381-506

    Plasmid

    #19853

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 381 to 506

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

24. pcDNA5FRT/TO PP1gamma-2*Strep

    Plasmid

    #113506

    Purpose

    Expresses dual Strep-tagged PP1gamma in mammalian cells

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    PP1gamma (PPP1CC Human)
    

    Use

    Tags

    Strep-Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. 1029-AIDx-CP

    Plasmid

    #135341

    Purpose

    Expression of circular permutant nSpyCas9 at amino acid position 1029 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    CP 1029 nSpyCas9-hAIDx
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. AB.pCCL.sin.cPPT.U6.miR-145-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46589

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-145 Decoy (MIR145, Mir145a Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA3-mCherry-mCherry-FLARE-EKAR-EV

    Plasmid

    #123341

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMGF157

    Plasmid

    #96972

    Purpose

    ish1-mCherry insertion cassette with Hygro resistance gene

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + hygro…

    Promoter

    N/A

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pET151-Vps4(101-437)-Hcp1 K321A/R325A

    Plasmid

    #89287

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 and Ar…

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. myc-Med4 pBacPAK8

    Plasmid

    #15364

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    Med4 (MED4 N/A, Human)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only