Addgene

We narrowed to 16,086 results for: IGH@

Showing: 5001 - 5020 of 16086 results

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UVR8-GFPCaaX

Plasmid

#49805

Purpose

expresses UVR8 fused to GFP with a prenylation motif for membrane targeting

Depositor

Matthew Kennedy

Article

Chen et al J Cell Biol. 2013 May 13;201(4):631-40.

Insert

UVR8-GFPCaax (UVR8 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCZGY2139

Plasmid

#74111

Purpose

Membrane targeted PH-miniSOG entry clone for efficient ablation of cells when expressed under desired promoter.

Depositor

Andrew Chisholm

Article

Xu et al Sci Rep. 2016 Feb 10;6:21271. doi: 10.10

Insert

miniSOG

Use

Gateway topo cloning

Tags

PH

Expression

Mutation

Promoter

Available since

April 8, 2016

Availability

Academic Institutions and Nonprofits only

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TAL2084

Plasmid

#39452

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-48-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pENTR_TiTRE

Plasmid

#172128

Purpose

Entry vector for tightly controlled gene expression by a tetracycline-responsive promoter

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Use

Entry vector

Tags

Expression

Mammalian

Mutation

Promoter

tight TRE

Available since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2052

Plasmid

#39410

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-27-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only
pBFv-U6.2B

Plasmid

#138401

Purpose

gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Insert

U6-2

Use

Tags

Expression

Insect

Mutation

Promoter

U6-2

Available since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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TAL2080

Plasmid

#39450

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-47-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2060

Plasmid

#39418

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-31-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pHR: pPGK mCherry-HP1A

Plasmid

#174115

Purpose

Expression of mCherry-HP1A

Depositor

Stanley Qi

Article

Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

Insert

mCherry-HP1A

Use

Lentiviral

Tags

Expression

Mutation

Promoter

PGK

Available since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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TAL2092

Plasmid

#39426

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-35-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
TAL2032

Plasmid

#39390

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-17-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pBXC3H

Plasmid

#47068

Purpose

FX cloning E.coli expression vector with araBAD promoter and C-terminal 3C protease cleavage site and 10x His tag

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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p7XC3GH

Plasmid

#47066

Purpose

FX cloning E.coli expression vector with T7 promoter and C-terminal 3C protease cleavage site, GFP and 10x His tags

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site), GFP, and dec…

Expression

Bacterial

Mutation

Promoter

T7

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pDONR221-Hyg

Plasmid

#48994

Purpose

Gateway adapted transfer of Hygromycin resistance gene

Depositor

Rick Tarleton

Article

Xu et al BMC Microbiol. 2009 May 11;9:90. doi: 10

Insert

hygromycin B phosphotransferase

Use

Gateway attb1 & attb2

Tags

Expression

Mutation

Promoter

Available since

April 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3-intein/Src

Plasmid

#71406

Purpose

Expresses Src inserted with a Npu DnaE intein (F1 construct)

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Src active kinase domain inserted with Npu DnaE intein

Use

Tags

Expression

Mammalian

Mutation

Promoter

pCMV

Available since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only
pGST1-TOM1-VHS

Plasmid

#44423

Depositor

James Hurley

Article

Ren et al EMBO J. 2010 Mar 17;29(6):1045-54. Epub

Insert

TOM1-VHS (1-152) (TOM1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pNL-EGFP/TREPittdU3

Plasmid

#18659

Depositor

Jakob Reiser

Article

Pluta et al J Gene Med. 2005 Jun . 7(6):803-17.

Insert

EGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 8, 2008

Availability

Academic Institutions and Nonprofits only

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TAL2094

Plasmid

#39428

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-36-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 5, 2012

Availability

Academic Institutions and Nonprofits only
pBXNH3

Plasmid

#47067

Purpose

FX cloning E.coli expression vector with araBAD promoter and N-terminal 10x His tag and 3C protease cleavage site

Depositor

Raimund Dutzler, Eric Geertsma

Article

Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.

Type

Empty backbone

Use

Tags

3C protease site (PreScission site) and decaHis (…

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

Jan. 15, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL2072

Plasmid

#39442

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

eGFP-TALEN-43-Left

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only