We narrowed to 5,570 results for: SPI

1. TOPO-EXOC3 (-1592 to -1046)

   Plasmid

   #226444

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1592 to -1046) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1592 to -1046) (EXOC3 Human)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TOPO-EXOC3 (-1591 to -1009)

   Plasmid

   #226443

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1591 to -1009) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1591 to -1009) (EXOC3 Human)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. TOPO-EXOC3 (-1701 to -1594)

   Plasmid

   #226442

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1701 to -1594) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1701 to -1594) (EXOC3 Human)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TOPO-EXOC3 (-1592 to -1444)

   Plasmid

   #226445

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1592 to -1444) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1592 to -1444) (EXOC3 Human)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. TOPO-EXOC3 (-1557 to-1444)

   Plasmid

   #226447

   Purpose

   For subcloning of human EXOC3 promoter (base pairs -1557 to-1444) or for assays using M13 phage

   Depositor

   Glen Borchert

   Article

   DeMeis et al Nucleic Acids Res. 2025 Jan 11;53(2):gka

   Insert

   EXOC3 (-1557 to-1444) (EXOC3 Human)
   

   Use

   Cloning vector

   Tags

   Expression

   Mutation

   No

   Promoter

   Available since

   July 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pHis-TEV-Nsp15-E266Q

   Plasmid

   #218803

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-E266Q (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-E266Q (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   E266Q (G to C)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHis-TEV-Nsp15-V66L

   Plasmid

   #219524

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-V66L (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-V66L (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   V66L (G to C)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHis-TEV-Nsp15-A81V

   Plasmid

   #219525

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-A81V (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-A81V (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   A81V (C to T)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHis-TEV-Nsp15-D183N

   Plasmid

   #219526

   Purpose

   E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-D183N (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

   Depositor

   Debra Hansen

   Article

   Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

   Insert

   Nsp15-D183N (N Severe acute respiratory syndrome coronavirus 2)
   

   Use

   Tags

   TEV protease cleavable His6-tag

   Expression

   Bacterial

   Mutation

   D183N (G to A)

   Promoter

   Available since

   May 27, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHis-TEV-Nsp15-H234A

    Plasmid

    #219529

    Purpose

    E. coli plasmid (T7lac promoter) for full-length, SARS CoV-2 Nsp15-H234A (uridine-specific nidoviral endoribonuclease, expression-optimized gene) with N-terminal TEV protease cleavable His6-tag

    Depositor

    Debra Hansen

    Article

    Sonowal et al Protein Sci. 2025 Jun;34(6):e70115. doi:

    Insert

    Nsp15-H234A (N Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    TEV protease cleavable His6-tag

    Expression

    Bacterial

    Mutation

    H234A (CAC to GCG)

    Promoter

    Available since

    May 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 7AA-aSyn-pRK172

    Plasmid

    #236197

    Purpose

    This plasmid expresses human α-synuclein with a 7-amino acid insertion (MAAAEKT) in the pRK172 backbone for bacterial expression in E. coli. The insertion corresponds to the JOS mutation.

    Depositor

    Sjors Scheres, Yang Yang

    Article

    Yang et al Acta Neuropathol. 2023 May;145(5):561-57

    Insert

    SNCA (SNCA Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    insertion os MAAAEKT at residue 22

    Promoter

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCBh-T7-SpG-P2A-mTagBFP2(RAS3583)

    Plasmid

    #223110

    Purpose

    pCBh Human expression plasmid for SpG with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpG-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCBh-T7-SpCas9-P2A-mTagBFP2(RAS3575)

    Plasmid

    #223111

    Purpose

    pCBh Human expression plasmid for SpCas9 with with C-term BPNLS-P2A-mTagBFP2

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized nuclease SpCas9-P2A-mTagBFP2
    

    Use

    CRISPR

    Tags

    BPNLS-3xFLAG-P2A-mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and T7

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG-SpCas9-P2A-EGFP (MSP2582)

    Plasmid

    #223067

    Purpose

    pCAG human codon optimized wild-type SpCas9 expression plasmid with C-terminal NLS(SV40)-3xFLAG-P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Silverstein et al Nature. 2025 Apr 22. doi: 10.1038/s41586

    Insert

    human codon optimized SpCas9-P2A-EGFP
    

    Use

    CRISPR

    Tags

    NLS(SV40)-3xFLAG-P2A-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 23, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRSF_his6_sumo_SARS_CoV2_NSP14_D90A/E92A

    Plasmid

    #213527

    Purpose

    Expresses NSP14 of SARS-CoV-2 with two point mutations D90A and E92A

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    NSP14 of SARS-CoV-2 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    D90A and E92A

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRSF_his6_sumo_hRNMT_RAM

    Plasmid

    #213528

    Purpose

    Expresses the human RNMT-RAM complex

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Inserts

    human RNMT (RNMT Human)
    RAM
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRSF_his6_sumo_SARS_CoV2_NSP14

    Plasmid

    #213518

    Purpose

    Expresses NSP14 of SARS-CoV-2

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    SARS-CoV-2 NSP14 (ORF1ab SARS-CoV-2)
    

    Use

    Tags

    N-terminal His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRSF_his6_sumo_OC43_NSP14

    Plasmid

    #213519

    Purpose

    Expresses NSP14 of hCoV OC43

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV OC43 NSP14 (ORF1ab hCoV OC43)
    

    Use

    Tags

    His-SUMO tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRSF_his6_sumo_NL63_NSP14

    Plasmid

    #213520

    Purpose

    Expresses NSP14 of hCoV NL63

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV NL63 NSP14 (ORF1ab hCoV NL63)
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRSF_his6_sumo_229E_NSP14

    Plasmid

    #213521

    Purpose

    Expresses NSP14 of hCoV 229E

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Nature. 2024 Dec 11. doi: 10.1038/s41586

    Insert

    hCoV 229E NSP14
    

    Use

    Tags

    His6-sumo

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge