We narrowed to 14,476 results for: nts

1. pDGE661

   Plasmid

   #153259

   Purpose

   M5 shuttle vector containing the Solanum lycopersicum U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

   Depositor

   Johannes Stuttmann

   Article

   Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   Aug. 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTRANS_263

   Plasmid

   #91127

   Purpose

   Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   35S

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA3_AKAR5Δ

   Plasmid

   #119912

   Purpose

   Mammalian expression of the AKAR5Δ sensor with N-terminal His tag for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

   Depositor

   Haining Zhong

   Article

   Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

   Insert

   AKAR5Δ
   

   Tags

   6xHis

   Expression

   Mammalian

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET:GCAT:GFP

   Plasmid

   #60736

   Purpose

   A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'GCAT' between -11 and -8.

   Depositor

   Matthew Bennett

   Article

   Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

   Insert

   GFP
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   Nov. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. KG#470

   Plasmid

   #110930

   Purpose

   Expresses proteins pan-neuronally in C. elegans (entire nervous system)

   Depositor

   Kenneth Miller

   Article

   Schade et al Genetics. 2005 Feb;169(2):631-49. doi: 1

   Inserts

   rab-3 promoter
   unc-54 3' control region
   

   Expression

   Bacterial

   Available Since

   June 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. FusionRed-BaxAlpha-C-15

   Plasmid

   #56101

   Purpose

   Localization: , Excitation: 580, Emission: 608

   Depositor

   Michael Davidson

   Article

   Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

   Insert

   BaxAlpha
   

   Tags

   FusionRed

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pFLIPglu-3.2mDelta13

   Plasmid

   #12992

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16A S112A Delta13
   

   Use

   Plant nanosensor

   Tags

   ECFP and EYFP

   Expression

   Plant

   Mutation

   F16A, S112A in binding protein

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

8. pHJ18

   Plasmid

   #48357

   Purpose

   Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, Puromycin//Gancyclovir selectable

   Depositor

   George Cross

   Article

   Kim et al Mol Biochem Parasitol. 2013 Aug 13;191(1

   Insert

   Partial beta-TUB followed by 5' ALD UTR-loxP-SAS-PUR-Ty1-TK-loxP-3' ALD UTR
   

   Use

   Cre/Lox; Knockout in t. brucei

   Available Since

   Jan. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFLIPglu-170nDelta13

   Plasmid

   #12989

   Depositor

   Wolf Frommer

   Article

   Deuschle et al Plant Cell. 2006 Sep . 18(9):2314-25.

   Insert

   FLIPglu F16F Delta13
   

   Use

   Plant nanosensor

   Tags

   ECFP and EYFP

   Expression

   Plant

   Mutation

   WT binding protein (no F16A)

   Available Since

   Jan. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pDest_pK7WG2_pRPS5A_CTP OleGFP

    Plasmid

    #171723

    Purpose

    ptpTALECD vector assembly, Destination vector;

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2
    

    Expression

    Plant

    Available Since

    Aug. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. E2_pENTR_R4R3_T CTP

    Plasmid

    #171736

    Purpose

    ptpTALECD vector assembly, Entry clone 2 with attR4 and R3

    Depositor

    Shin-ichi Arimura

    Article

    Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

    Insert

    A terminator of Arabidopsis heat shock protein, RPS5A promoter, and RecA1 plastid targeting signal
    

    Use

    Multisite gateway entry vector

    Available Since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEE389

    Plasmid

    #149287

    Purpose

    T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_TrunFT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 20, 2020

    Availability

    Academic Institutions and Nonprofits only

13. pEE387

    Plasmid

    #149285

    Purpose

    T-DNA encoding TRV2 with FT augmented gRNA targeting NbAG

    Depositor

    Daniel Voytas

    Article

    Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

    Insert

    p35S:TRV2_NbAGsgRNA1_FT:tNos
    

    Expression

    Plant

    Promoter

    35S

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LLP762_L2pV1_OR-OCS_TCTP-Flp_s35S-Cre

    Plasmid

    #192421

    Purpose

    To act as the 2-input state of the OR gate.

    Depositor

    Ryan Lister

    Article

    Lloyd et al Nat Biotechnol. 2022 Jul 4. pii: 10.1038

    Insert

    Act2::FRT-loxP-OCS-FRT-loxP::Rluc
    

    Use

    Synthetic Biology

    Tags

    PEST

    Expression

    Plant

    Available Since

    Nov. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Bov.hsc70.T77.A1-386.wt

    Plasmid

    #12534

    Depositor

    David McKay

    Article

    Wilbanks et al J Biol Chem. 1994 Apr 29. 269(17):12893-

    Insert

    Bovine 70 kDa heat shock protein ATPase fragment
    

    Expression

    Bacterial

    Mutation

    deletion of residues 387-650 of bovine Hsc70 prot…

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET:ACAT:GFP

    Plasmid

    #60740

    Purpose

    A modified pET28 driving expression of GFP, biobrick part Bba_E0040. No LacO site downstream of PT7. T7 promoter has base pairs 'ACAT' between -11 and -8.

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Nov. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pYPQ292-D570A

    Plasmid

    #129679

    Purpose

    Gateway entry clone for CRISPR-Cas12b systems

    Depositor

    Yiping Qi

    Article

    Ming et al Nat Plants. 2020 Mar;6(3):202-208. doi:

    Insert

    AaCas12b
    

    Use

    CRISPR; Gateway compatible aacas12b entry clone

    Expression

    Plant

    Mutation

    D570A; AaCas12b is rice codon optimized

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5_tAKARγ

    Plasmid

    #119915

    Purpose

    Mammalian expression of the tAKARγ sensor for detecting PKA activity using two-photon fluorescence lifetime imaging microscopy (2pFLIM)

    Depositor

    Haining Zhong

    Article

    Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

    Insert

    tAKARγ
    

    Expression

    Mammalian

    Available Since

    April 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAGM55361

    Plasmid

    #153227

    Purpose

    Control plasmid for knockout of AP3 gene

    Depositor

    Sylvestre Marillonnet

    Article

    Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

    Insert

    Cas9, guide RNA for TRY and CPC and FAST marker
    

    Use

    Synthetic Biology

    Available Since

    Aug. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pDGE509

    Plasmid

    #153281

    Purpose

    End-linker for cloning 3 sgRNA arrays into any recipient vector

    Depositor

    Johannes Stuttmann

    Article

    Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

    Insert

    linker
    

    Use

    CRISPR

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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