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pDONR221_SLC38A3

Plasmid

#132142

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC38A3 (SLC38A3 Human)

Expression

Mammalian

Available Since

Nov. 25, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLEX-HA-birA*-K-Ras(wildtype)-IRES-Puro

Plasmid

#120561

Purpose

Expresses HA-birA*-K-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

KRAS4B (KRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 29, 2019

Availability

Academic Institutions and Nonprofits only

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NTHL1-mCherry

Plasmid

#215145

Purpose

Expresses NTHL1-mCherry in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

NTHL1 (NTHL1 Human)

Use

Retroviral

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

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pmCherry-TEAD4-IDR

Plasmid

#166451

Purpose

Expresses fusion of mCherry and TEAD4-IDR

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mCherry, TEAD4-IDR(89-230) (TEAD4 Human)

Expression

Mammalian

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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TFORF0929

Plasmid

#143081

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ATOH8 (ATOH8 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC27A4

Plasmid

#132283

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC27A4 (SLC27A4 Human)

Expression

Mammalian

Available Since

Nov. 14, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pGL3 1 kb prom. + Enhancer CD274

Plasmid

#107005

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Mutation

N.B. There are two published SNPs in this region …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-PPO-mScarlet

Plasmid

#139503

Purpose

Expresses mScarlet-tagged parapinopsin in mammalian cells for photoswitchable control of inhibitory GPCR signaling cascades.

Depositor

Michael Bruchas

Article

Copits et al Neuron. 2021 Jun 2;109(11):1791-1809.e11

Insert

parapinopsin

Tags

mScarlet following Gly-Gly-Ser linker

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX-uORF-HA-birA*-H-Ras(wildtype)-IRES-Puro

Plasmid

#120559

Purpose

Expresses HA-birA*-H-Ras wildtype fusion protein in mammalian cells & for virus production

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Insert

HRAS (HRAS Human)

Use

Lentiviral

Tags

HA-birA*

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pET15-ZmPYK

Plasmid

#220230

Purpose

Inducible expression of Zymomonas mobilis pyruvate kinase

Depositor

Aron Fenton, Liskin Swint-Kruse

Article

Swint-Kruse et al Database (Oxford). 2023 May 3;2023:baad0

Insert

Zymomonas mobilis pyruvate kinase (pyk, ZMO0152 Zymomonas mobilis)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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NanoBRET BiBRET Vector, NanoLuc-HRAS WT-CRAF RBD-HaloTag

Plasmid

#236861

Purpose

Express NanoLuc(R)-HRAS WT-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

HRAS (HRAS Human)
cRAF(RBD) (RAF1 Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3/hArf3(WT)-mCherry

Plasmid

#79420

Purpose

Expresses C-terminally mCherry-tagged Arf3(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf3 (ARF3 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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PLKO.1-YAP shRNA2

Plasmid

#166485

Purpose

Human YAP RNAi

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

shRNA for human YAP (YAP1 Human)

Use

Lentiviral

Available Since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3 3'UTR reporter WT 1.3 kb CD274 Hs 3'UTR Final

Plasmid

#107009

Purpose

Modified Luciferase expression vector

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

CD274 (CD274 Human)

Use

Luciferase

Expression

Mammalian

Available Since

March 23, 2018

Availability

Academic Institutions and Nonprofits only

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NanoBRET BiBRET Vector HaloTag-HRAS WT-BRAF RBD-NanoLuc

Plasmid

#236850

Purpose

Express HaloTag(R)-HRAS WT-BRAF RBD-NanoLuc(R) in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Inserts

HRAS (HRAS Human)
BRAF (RBD) (BRAF Human)

Tags

HaloTag (R) and NanoLuc (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pUb FLAG-Human IntS6

Plasmid

#198408

Purpose

Expresses FLAG-tagged human IntS6

Depositor

Jeremy Wilusz

Article

Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

Insert

DDX26 (INTS6 Human)

Tags

3x FLAG

Expression

Insect

Promoter

Ubi-p63e

Available Since

Nov. 29, 2023

Availability

Academic Institutions and Nonprofits only

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RCAS-Flag-BRAFV600E

Plasmid

#126615

Purpose

Retroviral expression of Flag tagged human oncogenic BRAFV600E

Depositor

Yi Li

Article

Bu et al Cancer Res. 2019 Jan 1;79(1):61-71. doi:

Insert

BRAF (BRAF Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

V600E

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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p3xFLAG CMV14 / BRAF-D594G

Plasmid

#131719

Purpose

Mammalian Expression of Flag tagged BRAF

Depositor

Jacques De Grève

Article

Noeparast et al Oncotarget. 2016 Aug 26;8(36):60094-6010

Insert

BRAF (BRAF Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

D594G

Promoter

CMV

Available Since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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AcGFP-PMS2

Plasmid

#215124

Purpose

Expresses AcGFP-PMS2 in mammalian cells from a retroviral vector.

Depositor

Michele Pagano

Article

Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

Insert

PMS2 (PMS2 Human)

Use

Retroviral

Tags

AcGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ Myc2-mouse TTP aa S52 S178 MUT

Plasmid

#107001

Purpose

Expresses epitope-tagged double-mutant murine TTP

Depositor

Julian Downward

Article

Coelho et al Immunity. 2017 Dec 19;47(6):1083-1099.e6

Insert

tristetraprolin (Zfp36 Mouse)

Tags

Double Myc peptide epitope tag

Expression

Mammalian

Mutation

Changed Ser52 to Ala and Ser178 to Ala

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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