We narrowed to 9,203 results for: sacs

1. pCin4-3xFLAG-MKL1 L272A

   Plasmid

   #19850

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 L272A (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Leucine 272 to Alanine

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

2. pCin4-3xFLAG-MKL1 D444-630

   Plasmid

   #19840

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 D444-630 (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   deleted amino acids 444-630

   Promoter

   Available since

   March 16, 2009

   Availability

   Academic Institutions and Nonprofits only

3. pCin4-3xFLAG-MKL1 S449A

   Plasmid

   #19842

   Depositor

   Ron Prywes

   Article

   Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

   Insert

   MKL1 S449A (MRTFA Human)
   

   Use

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   changed Serine 449 to Alanine

   Promoter

   Available since

   Dec. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

4. pAG415-GPDp-EGFP-rap1-K651R

   Plasmid

   #84536

   Purpose

   This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

   Depositor

   Jorrit Enserink

   Article

   Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

   Insert

   RAP1 (RAP1 Budding Yeast)
   

   Use

   Tags

   EGFP

   Expression

   Yeast

   Mutation

   K651R

   Promoter

   GPD

   Available since

   Dec. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pRS426-TUB1-internal6xHis - human TUBA1a Cterminal tail - delta Y

   Plasmid

   #60393

   Purpose

   Expression of chimeric yeast alpha tubulin (TUB1) - internal 6xHis with human alpha tubulin Cterminal tail (TUBA1a) without Cterminal tyrosine (delta Y)

   Depositor

   Ron Vale

   Article

   Sirajuddin et al Nat Cell Biol. 2014 Apr;16(4):335-44. do

   Insert

   TUB1 (TUB1 Synthetic, Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   internal 6xHis (see supplement figure 4)

   Promoter

   GAL

   Available since

   Jan. 26, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pMGF170

   Plasmid

   #96997

   Purpose

   ish1-mCherry insertion cassette with NAT resistance gene

   Depositor

   Wesley Sundquist, Adam Frost

   Article

   Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

   Insert

   ish1 (ish1 Fission Yeast)
   

   Use

   Tags

   GST and mCherry

   Expression

   Bacterial

   Mutation

   ish1 C-terminal domain fused with mCherry + NAT r…

   Promoter

   N/A

   Available since

   Aug. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. HA-PRAS40 F129A

   Plasmid

   #86763

   Purpose

   N-terminal HA-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   human PRAS40 (AKT1S1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F129A

   Promoter

   CMV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. HA-PRAS40 W10A

   Plasmid

   #86761

   Purpose

   N-terminal HA-tagged protein expression in mammalian cells

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   human PRAS40 (AKT1S1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   W10A

   Promoter

   CMV

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. tet-pLKO shMKP7.v1 puro

   Plasmid

   #87794

   Purpose

   Expresses an inducible short hairpin targeting human MKP7 sequence

   Depositor

   Kevin Janes

   Article

   Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

   Insert

   shMKP7 (DUSP16 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE tight

   Available since

   March 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. MDH1-miR-221SM-PGK-GFP-Blast

    Plasmid

    #31520

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-221 (Mir221 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 21, 2011

    Availability

    Academic Institutions and Nonprofits only

11. tet-pLKO shDUSP19 puro

    Plasmid

    #87796

    Purpose

    Expresses an inducible short hairpin targeting human DUSP19 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shDUSP19 (DUSP19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFB-Flag-MyoD(QQQ)-E12

    Plasmid

    #26003

    Depositor

    Jeffrey Dilworth

    Article

    Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

    Insert

    MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)
    

    Use

    Baculovirus transfer vector

    Tags

    Flag

    Expression

    Mutation

    Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

    Promoter

    Available since

    Sept. 8, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MDH1-miR-196a-1SM-PGK-GFP-Blast

    Plasmid

    #31510

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-196a1 (Mir196a-1 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

14. AB.pCCL.sin.cPPT.U6.miR-382-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46618

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-382 Decoy (MIR382, Mir382 Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

15. AB.pCCL.sin.cPPT.U6.miR-496-Decoy.hPGK.GFP.WPRE

    Plasmid

    #46622

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-496 Decoy (MIR496, Mir496a Human, Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 29, 2013

    Availability

    Academic Institutions and Nonprofits only

16. Hoxa4-3'UTR

    Plasmid

    #31524

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    Hoxa4-3'UTR (Hoxa4 Mouse)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

17. Rev CDK9 (D167N) (P#858)

    Plasmid

    #14635

    Depositor

    Matija Peterlin

    Article

    Fujinaga et al J Virol. 1998 Sep . 72(9):7154-9.

    Insert

    cdk9 D167N (CDK9 Human)
    

    Use

    Tags

    Rev

    Expression

    Mammalian

    Mutation

    D167N. Kinase negative mutant.

    Promoter

    Available since

    June 30, 2008

    Availability

    Academic Institutions and Nonprofits only

18. MDH1-miR-324SM-PGK-GFP-Blast

    Plasmid

    #31518

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Insert

    miR-324 (Mir324 Mouse)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    seed mutant control

    Promoter

    Available since

    Sept. 19, 2011

    Availability

    Academic Institutions and Nonprofits only

19. GST-MED9

    Plasmid

    #15408

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    MED9 (Med9 Mouse)
    

    Use

    Tags

    GST and His

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    Available since

    Aug. 10, 2007

    Availability

    Academic Institutions and Nonprofits only

20. FLAG-Med9 pBacPAK8

    Plasmid

    #15363

    Depositor

    Ronald Conaway, Joan Conaway

    Article

    Tomomori-Sato et al J Biol Chem. 2004 Feb 13. 279(7):5846-51

    Insert

    Med9 (Med9 Mouse)
    

    Use

    Baculovirus construction

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

21. pBB3-ScVip1-536-1107-delta848-918

    Plasmid

    #238313

    Purpose

    ScVip1-536-1107-delta848-918 protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-delta848-918

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pBB3-ScVip1-536-1107-R547A-H548A-R551A

    Plasmid

    #238317

    Purpose

    ScVip1-536-1107-R547A-H548A-R551A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-R547A-H548A-R551A

    Promoter

    Available since

    July 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBB3-ScVip1-536-1107-K554A-K556A-K644A

    Plasmid

    #238328

    Purpose

    ScVip1-536-1107-K554A-K556A-K644A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-K554A-K556A-K644A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pBB3-ScVip1-536-1107-P553V-G646V-G647A

    Plasmid

    #238322

    Purpose

    ScVip1-536-1107-P553V-G646V-G647A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-P553V-G646V-G647A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pBB3-ScVip1-536-1107-K817S-D819A-S821A

    Plasmid

    #238326

    Purpose

    ScVip1-536-1107-K817S-D819A-S821A protein expression in insect cells

    Depositor

    Michael Hothorn

    Article

    Raia et al Nat Commun. 2025 Feb 19;16(1):1753. doi:

    Insert

    VIP1 (VIP1 Budding Yeast)
    

    Use

    Tags

    His10-Tag, Twin-Strep-tag, TEV

    Expression

    Insect

    Mutation

    ScVip1-536-1107-K817S-D819A-S821A

    Promoter

    Available since

    June 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PCDNA3.1:mTRESK

    Plasmid

    #224771

    Purpose

    In vitro transcription for Oocyte expression of mTRESK

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 18 (Kcnk18 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

    Plasmid

    #225228

    Purpose

    Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (25Q)
    

    Use

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

    Plasmid

    #225229

    Purpose

    Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (46Q)
    

    Use

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLViP-LifeAct-TagRFPT

    Plasmid

    #229700

    Purpose

    Lentiviral expression of LifeAct-TagRFPT in mammalian cells

    Depositor

    Alpha Yap

    Article

    Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

    Insert

    Synthetic construct LifeAct-mTurquoise gene
    

    Use

    Lentiviral

    Tags

    TagRFPT

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. ABEmax-Puro V2

    Plasmid

    #226955

    Purpose

    ABEmax plasmid enabling A:T to G:C base editing using a single plasmid.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge