Addgene

Showing: 5021 - 5040 of 16299 results

Results per page:

Sort results by:

pJY-RpABE

Plasmid

#112872

Purpose

binary vector for ABE expression in Arabidopsis

Depositor

Jin-Soo Kim, Jae-Young Yun

Article

Kang et al Nat Plants. 2018 Jun 4. pii: 10.1038/s41

Inserts

RPS5A promoter
ABE7.10
U6-sgRNA cassette

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

Available since

Sept. 12, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pDL17

Plasmid

#106483

Purpose

pDL17 is based on conditionally replicating backbone derived from pQE-30 and expresses gam, bet and exo genes under control of PrhaB promoter for efficient dsDNA integration into E. coli chromosome

Depositor

Sergey Sineoky

Article

Bubnov et al J Microbiol Methods. 2018 Jun 7. pii: S0

Inserts

gam
bet
exo
lacI
rhaR
rhaS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-pMecp2-dSaCas9-VP64-pU6-sgRNA

Plasmid

#158972

Purpose

Vector C encodes pAAV-pMecp2-dSaCas9-VP64-spA-pU6-sgRNA (BsaI) transgenes for AAV packaging and expression of CRISPR activator in neurons

Depositor

Chung Tin

Article

Lau et al Mol Ther Nucleic Acids. 2019 Jun 7;16:63

Insert

dSaCas9-VP64

Use

AAV and CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

pMecp2

Available since

Sept. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a_SUMO_AcCas12n

Plasmid

#203808

Purpose

pET28a-based plasmid containing a T7-driven His6-SUMO-HRV3c-AcCas12n cassette. Kanamycin resistance.

Depositor

Quanjiang Ji

Article

Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

Insert

SUMO_AcCas12n

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Sox2-t2A-GFP

Plasmid

#127537

Purpose

Expresses human SOX2 and eGFP via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-pLKO shGATA6 puro

Plasmid

#72615

Purpose

Expresses an inducible short hairpin targeting human GATA6 sequence

Depositor

Kevin Janes

Article

Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

Insert

short hairpin GATA binding protein 6

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

TRE Tight

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Lyso-mCherry-Rheb(C181S)

Plasmid

#192438

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

Lyso-mCherry-Rheb(C181S) (RHEB Human)

Use

Tags

Full-length LAMP1 and mCherry

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available since

Dec. 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-mCherry-Rheb(C181S)

Plasmid

#192437

Purpose

Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.

Depositor

Jin Zhang

Article

Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

Insert

mCherry-Rheb(C181S) (RHEB Human)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Rheb Cys 181 mutated to Ser; abolishes C-terminal…

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pM123: pAAV-EFS-CasRx-control presgRNA

Plasmid

#166871

Purpose

AAV vector for expressing CasRx and control presgRNA for RNA-editing

Depositor

Guei-Sheung Liu

Article

Chuang et al Front Cell Dev Biol. 2021 Jun 11;9:66787

Inserts

U6-control presgRNA
RfxCas13d

Use

AAV and CRISPR

Tags

HA and NLS

Expression

Mammalian

Mutation

Promoter

EFS and U6

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA-1xHA CARM1 WT

Plasmid

#81118

Purpose

mammalian expression of CARM1 WT

Depositor

Sung Hee Baek

Article

Shin et al Nature. 2016 Jun 15. doi: 10.1038/nature

Insert

CARM1 (Carm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 28, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA flag PPAR gamma E499Q

Plasmid

#8896

Depositor

Bruce Spiegelman

Article

Hauser et al J Biol Chem. 2000 Jun 16. 275(24):18527-

Insert

PPAR gamma (Pparg Mouse)

Use

Tags

flag

Expression

Mammalian

Mutation

E499Q, a mutant in the AF2 domain. Binds PPARg l…

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pMito-Electra2

Plasmid

#184927

Purpose

Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A

Depositor

Kiryl Piatkevich

Article

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

COX8A-Electra2

Use

Tags

Electra2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pClgn1.1-Trim41-PA-1D4

Plasmid

#186339

Purpose

Express Trim41-PA-1D4 under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Article

Oura et al PLoS Genet. 2022 Jun 1;18(6):e1010241. d

Insert

Trim41 (Trim41 Mouse)

Use

Tags

1D4 and PA

Expression

Mammalian

Mutation

Promoter

pClgn1.1

Available since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pPW3754 : pUC-SpCas9_gRNA-HsIRE1-Cterm

Plasmid

#185676

Purpose

SpCas9 and gRNA targeting the C-terminus of HsIRE1

Depositor

Peter Walter

Article

Belyy et al Elife. 2022 Jun 22;11:e74342. doi: 10.75

Insert

ERN1 gRNA (ERN1 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

March 14, 2023

Availability

Academic Institutions and Nonprofits only
pEGFP-ATXN1-52Q

Plasmid

#32492

Depositor

Huda Zoghbi

Article

Cummings et al Nat Genet. 1998 Jun;19(2):148-54.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

insert contains a stretch of 52 Glutamines and no…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
DsRed-rab9 WT

Plasmid

#12677

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB9 (Homo sapiens) (RAB9A Human)

Use

Tags

DsRed and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pCDNA3 Flag Jnk2a2

Plasmid

#13755

Depositor

Roger Davis

Article

Gupta et al EMBO J. 1996 Jun 3. 15(11):2760-70.

Insert

Jnk2a2 (MAPK9 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
BPK3079

Plasmid

#78741

Purpose

Human expression plasmid for AsCpf1 guide RNA (need to clone in spacer into BsmBI sites)

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

AsCpf1 crRNA backbone, without spacer sequence

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
p2T-CMV-eA3A-BE5-NG-BlastR

Plasmid

#152998

Purpose

High purity C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-BE4 T44D+S45A-nSpCas9-UGI-UGI

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 24, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-Basic-IRES

Plasmid

#64784

Purpose

Luciferase reporter vector

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Type

Empty backbone

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge