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pEYFP-C1-PHF13 ∆24-40∆232-238

Plasmid

#246105

Purpose

Expression of human PHF13 with aa 24-40 and 232-238 deletion fused to mEYFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 ∆24-40∆232-238 (PHF13 Human)

Tags

mEYFP

Expression

Mammalian

Mutation

del aa 24 - 40 and 232 - 238

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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PEYFP-C1-PHF13∆PEST2

Plasmid

#246104

Purpose

Expression of human PHF13 with PEST2 deletion fused to mEYFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13∆PEST2 (PHF13 Human)

Tags

mEYFP

Expression

Mammalian

Mutation

del PEST2 ( del aa 141 - 189 )

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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PEYFP-C1-PHF13∆PEST1

Plasmid

#246103

Purpose

Expression of human PHF13 with PEST1 deletion fused to mEYFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13∆PEST1 (PHF13 Human)

Tags

mEYFP

Expression

Mammalian

Mutation

del PEST1 ( del aa 52 - 87 )

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEYFP-C1-PHF13 (150-300)

Plasmid

#246102

Purpose

Expression of human PHF13 (150-300) fused to mEYFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (150-300) (PHF13 Human)

Tags

mEYFP

Expression

Mammalian

Mutation

aa 151 - 300

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pEYFP-C1-PHF13∆NTD (del21-70)

Plasmid

#246100

Purpose

Expression of human PHF13 with N terminal domain deletion fused to mEYFP in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13∆NTD (del21-70) (PHF13 Human)

Tags

mEYFP

Expression

Mammalian

Mutation

del aa 21 - 70 , K20E

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV4-PHF13 (150-300)

Plasmid

#246097

Purpose

Expression of human PHF13 (150-300)with Flag-tag in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (150-300) (PHF13 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 151 - 300

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV4-PHF13 (1-150)

Plasmid

#246096

Purpose

Expression of human PHF13 (1-150) with Flag tag in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13 (1-150) (PHF13 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 1 - 150

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pFlag-CMV4-PHF13 (100-200)

Plasmid

#246095

Purpose

Expression of human PHF13 (100-200) with Flag-tag in mammalian cells

Depositor

Sarah Kinkley

Article

Rossi et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

PHF13(100-200) (PHF13 Human)

Tags

Flag

Expression

Mammalian

Mutation

aa 101 - 200

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pET28-His6-SUMO-Yes-SH2-AviTag

Plasmid

#214208

Purpose

Bacterial expression of SH2 domain of the Yes kinase with N-terminal Thrombin-cleavable 6xHis tag and SUMO and C-terminal AviTag for Streptavidin bead functionalization for binder selections

Depositor

Neel Shah

Article

Gagoski et al Protein Sci. 2025 Nov;34(11):e70317. doi

Insert

Yes kinase SH2 domain (YES1 Human)

Tags

6xHis, AviTag, and SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-myc-hSema4D-Q497P

Plasmid

#190655

Purpose

Expresses Human Sema4D with N terminal myc after signal peptide containing 497P mutation

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

myc

Expression

Mammalian

Mutation

497P

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-sp-flag-hSema4D-Q497P

Plasmid

#190657

Purpose

Expresses Human Sema4D with N terminal flag after signal peptide containing 497P mutation

Depositor

Andrew Newman, Victor Tarabykin

Article

Bessa et al Nat Commun. 2024 Aug 16;15(1):7059. doi:

Insert

Sema4D (SEMA4D Human)

Tags

flag

Expression

Mammalian

Mutation

497P

Available Since

July 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-DIO-ExRai-AKAR2-T/A

Plasmid

#171847

Purpose

Cre-dependent, synapsin promoter-driven expression of the negative control phosphomutant ExRai-AKAR2 T/A PKA biosensor in neurons.

Depositor

Richard Huganir

Article

Zhang et al Nat Chem Biol. 2020 Sep 28. pii: 10.1038

Insert

ExRai-AKAR2 T/A

Use

AAV

Expression

Mammalian

Mutation

T6A phospho-deficient mutation

Promoter

hSyn1

Available Since

July 15, 2025

Availability

Academic Institutions and Nonprofits only

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Str-KDEL_SBP-EGFP-CCR5-Cys3A

Plasmid

#222313

Purpose

Synchronize the trafficking of CCR5-Cys3A from the ER.

Depositor

Franck Perez

Article

Boncompain et al Sci Adv. 2019 Oct 16;5(10):eaax0821. doi

Insert

Streptavidin-KDEL and CCR5-Cys3A fused to SBP-EGFP (CCR5 Human)

Expression

Mammalian

Mutation

C321A, C323A, C324A

Promoter

CMV

Available Since

Feb. 4, 2025

Availability

Academic Institutions and Nonprofits only

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pLJC2-DHFR-3xFLAG

Plasmid

#217428

Purpose

Lentiviral overexpression of human DHFR

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_1

Plasmid

#217433

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 1 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-v1-sgABCC4_3

Plasmid

#217434

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 3 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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R2pH-LAMP-FRB

Plasmid

#225138

Purpose

R2pH-LAMP1-labelled lysosomes with FRB

Depositor

Massimiliano Stagi

Article

Cheetham-Wilkinson et al Sci Adv. 2025 Jan 3;11(1):eadr7325. doi:

Insert

Lamp1 (Lamp1 Mouse)

Expression

Mammalian

Available Since

Jan. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pSuper Retro GFP DGK zeta human

Plasmid

#224579

Purpose

To stably downregulate the expression of human DGK zeta in human cells

Depositor

Isabel Mérida

Article

Torres-Ayuso et al Oncogenesis. 2015 Aug 24;4(8):e164. doi:

Insert

sh RNAi Diacylglycerol kinase zeta human (GTPBP6 Human)

Use

RNAi and Retroviral

Promoter

H1

Available Since

Sept. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR223-TAF5-∆ex8_WobbleMut

Plasmid

#209054

Purpose

Gateway-compatible entry vector, with insert of the TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNA

Depositor

Thomas Gonatopoulos-Pournatzis

Article

Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

Insert

TAF5 deltaexon-8

Use

Gateway entry vector

Expression

Mammalian

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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lenti-EGFR-L858R-T790M-dual-nick sgRNA

Plasmid

#214101

Purpose

Lentiviral vector expressing nicking sgRNAs for the induction of EGFR L858R and T790M mutations, through tandem U6 expression of the two sgRNAs using independent U6 promoters.

Depositor

Ting Zhou

Article

Wu et al Nat Commun. 2024 Dec 30;15(1):10824. doi

Insert

EGFR L858R nicking sgRNA/EGFR T790M nicking sgRNA

Use

Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

June 6, 2024

Availability

Academic Institutions and Nonprofits only

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