We narrowed to 9,193 results for: Pol;

1. pTU2S-b (p15A origin)

   Plasmid

   #74089

   Purpose

   Secondary module (BpiI) site in pTU2-a backbone with p15A (low-copy) origin of replication

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Golden Gate destination vector - Level 2, 3 TU's with secondary module and p15A origin
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   None

   Available Since

   April 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. A3Bmax

   Plasmid

   #207167

   Purpose

   Express full-length human A3B in a BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B (APOBEC3B Human)
   

   Tags

   NLS and NLS, 6x His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pOPINK-TRABID (NZF1, aa 1-33)

   Plasmid

   #65437

   Purpose

   Expresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coli

   Depositor

   David Komander

   Article

   Michel et al Mol Cell. 2015 Apr 2;58(1):95-109. doi:

   Insert

   TRABID (ZRANB1 Human)
   

   Tags

   His6-GST

   Expression

   Bacterial

   Mutation

   only codes for amino acids 1-33 of TRABID, which …

   Promoter

   T7

   Available Since

   July 27, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pLV.PARP1#5

   Plasmid

   #14548

   Depositor

   Didier Trono

   Article

   Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

   Insert

   PARP1 shRNA (PARP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

5. pGBW-m4046799

   Plasmid

   #145577

   Purpose

   Bacterial Expression plasmid for SARS-CoV-2 RNA-dependent RNA polymerase

   Depositor

   Ginkgo Bioworks, Benjie Chen

   Insert

   SARS-CoV-2 RNA-dependent RNA polymerase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
   

   Tags

   Cleavable TEV;6xHIS

   Expression

   Bacterial

   Mutation

   wt

   Promoter

   Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

   Available Since

   April 28, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. GAL FLAG25QDProCFPp303

   Plasmid

   #15584

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 25Q Delta Pro (HTT Human)
   

   Tags

   CFP and FLAG

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Available Since

   Aug. 15, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pLV.PARP1#4

   Plasmid

   #14547

   Depositor

   Didier Trono

   Article

   Ariumi et al J Virol. 2005 Mar . 79(5):2973-8.

   Insert

   PARP1 shRNA (PARP1 Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   March 27, 2007

   Availability

   Academic Institutions and Nonprofits only

8. GAL FLAG46QDProGFP p306

   Plasmid

   #15832

   Depositor

   Susan Lindquist

   Article

   Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   htt 46Q Delta Pro (HTT Human)
   

   Tags

   FLAG and GFP

   Expression

   Yeast

   Mutation

   Proline-rich region removed

   Available Since

   Oct. 5, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pCellFree_G03 TGFB1

   Plasmid

   #67080

   Purpose

   Standard for cell-free expression benchmarking.

   Depositor

   Kirill Alexandrov

   Article

   Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

   Insert

   TGFB1 (TGFB1 Human)
   

   Use

   Cell-free expression

   Tags

   EGFP and His

   Promoter

   T7

   Available Since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pGoldyTALEN-VIM-TAL-2

    Plasmid

    #62852

    Purpose

    TALEN mediated gene editing at human VIM locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pGoldyTALEN-VIM-TAL-1

    Plasmid

    #62855

    Purpose

    TALEN mediated gene editing at human VIM locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. GAL 46Q+ProGFPp416

    Plasmid

    #15581

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 46Q + Pro (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

13. GAL 25Q+ProGFPp416

    Plasmid

    #15580

    Depositor

    Susan Lindquist

    Article

    Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

    Insert

    htt 25Q + Pro (HTT Human)
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Proline-rich region present

    Available Since

    Aug. 15, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pPRIME-CMV-Neo-FF3

    Plasmid

    #11665

    Purpose

    3rd generation lentiviral transfer vector. pPRIME cloning plasmid with a CMV promoter controlling expression of Neomycin and miR30-based shRNA targeting firefly luciferase

    Depositor

    Stephen Elledge

    Article

    Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

    Insert

    FF3
    

    Use

    Lentiviral

    Tags

    Neo

    Expression

    Mammalian

    Available Since

    March 1, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTU2S-a (colE1 origin)

    Plasmid

    #74091

    Purpose

    Secondary module (BpiI) site in pTU2-a backbone with colE1 (medium-copy) origin of replication

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Golden Gate destination vector - Level 2, 2 TU's with secondary module and colE1 origin
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    None

    Available Since

    April 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pECFP-Rac1Y40C

    Plasmid

    #105293

    Purpose

    Control for FRET biosensor analysis - cannot bind downstream effectors

    Depositor

    Kenneth Yamada

    Article

    Petrie et al J Cell Biol. 2012 Apr 30;197(3):439-55.

    Insert

    Rac1 (RAC1 Human)
    

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    Y40C

    Available Since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMVTAG-NEMOL329P

    Plasmid

    #11971

    Depositor

    Jon Ashwell

    Article

    Wu et al Nat Cell Biol. 2006 Apr . 8(4):398-406.

    Insert

    NEMOL329P (IKBKG Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Leucine329 to Proline

    Available Since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

18. MAP2-CD4

    Plasmid

    #43916

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Insert

    CD4 (CD4 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    Human MAP2 promoter

    Available Since

    April 1, 2013

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-C-SLC35B2

    Plasmid

    #155037

    Purpose

    Transient expression of EGFP-SLC35B2

    Depositor

    Yihong Ye

    Article

    Zhang et al Proc Natl Acad Sci U S A. 2020 May 19;11

    Insert

    SLC35B2 (SLC35B2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. BirA*-HA-NES

    Plasmid

    #214473

    Purpose

    Lentiviral vector for expression in mammalian cells of the proximity ligase BirA(R118G) with a C-terminal HA tag followed by a nuclear exclusion sequence.

    Depositor

    Natalie Ahn

    Article

    Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

    Insert

    BirA(R118G)
    

    Use

    Lentiviral; Gateway

    Tags

    HA tag and NES (Nuclear Exclusion Sequence)

    Expression

    Mammalian

    Mutation

    R118G

    Promoter

    EF-1a

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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