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pcDNA3.1(+)MBII85

Plasmid

#67643

Purpose

MBII85 snoRNA expression cassett, contains snoRNA in natural intron surrounded by natural exons.

Depositor

Stefan Stamm

Article

Falaleeva et al Gene. 2015 Jul 26. pii: S0378-1119(15)00

Insert

MBII85 snoRNA

Expression

Mammalian

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pBN67

Plasmid

#86714

Purpose

DamID control plasmid for C. elegans

Depositor

Peter Askjaer

Article

Gonzalez-Aguilera et al Genome Biol. 2014 Feb 3;15(2):R21. doi:

Insert

Phsp16.41::GFP::MYCDam

Expression

Worm

Available Since

Feb. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pAG374-H2B-greenFAST

Plasmid

#160359

Purpose

Expresses H2B-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

H2B-greenFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pSopBWT

Plasmid

#112512

Purpose

PsopB driven sopB and sigE

Depositor

Olivia Steele-Mortimer

Article

Knodler et al Cell Microbiol. 2009 Nov;11(11):1652-70.

Insert

sopB/sigE operon

Expression

Bacterial

Promoter

PsopB

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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p63(1-29)-mCherry-N1

Plasmid

#67895

Purpose

Expresses of fusion of mCherry with the N-terminal 29 amino acids of p63RhoGEF

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

p63RhoGEF(1-29)-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn_NLS-His-rsCaMPARI-mRuby3

Plasmid

#122092

Purpose

AAV plasmid for expressing rsCaMPARI in neurons, nucleus localized

Depositor

Eric Schreiter

Article

Sha et al Elife. 2020 Sep 15;9. pii: 57249. doi: 1

Insert

rsCaMPARI

Use

AAV

Tags

NLS-His and mRuby3

Promoter

hsyn (synapsin-1)

Available Since

Oct. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pGSTag BimEL

Plasmid

#23103

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pGSTag Bim EL (Bcl2l11 Mouse)

Tags

GSTag

Expression

Bacterial

Available Since

Feb. 24, 2010

Availability

Academic Institutions and Nonprofits only
XE71 NES Beta-catenin GFP in CS2+

Plasmid

#16837

Depositor

Randall Moon

Article

Miller et al J Cell Biol. 1997 Oct 6. 139(1):229-43.

Insert

NES beta-catenin GFP (ctnnb1.L Frog)

Use

Xenopus expression

Tags

GFP

Available Since

March 14, 2008

Availability

Academic Institutions and Nonprofits only
pAAV-CAG-R-Hras

Plasmid

#180579

Purpose

ddRFP sensor of Hras

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddRFP sensor of Hras

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-G-Rac1

Plasmid

#180581

Purpose

ddGFP sensor of Rac1

Depositor

Won Do Heo

Article

Kim J et al Nat Commun. 2019 Jan 14;10(1):211. doi:

Insert

ddGFP sensor of Rac1

Use

AAV

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pI12

Plasmid

#24273

Depositor

Mariano Garcia-Blanco

Article

Baraniak et al Mol Cell Biol. 2003 Dec . 23(24):9327-37

Insert

modified adenovirus exons and introns

Use

T7 for in vitro transcription

Expression

Mammalian

Available Since

March 30, 2010

Availability

Academic Institutions and Nonprofits only

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pAcrF3

Plasmid

#89246

Purpose

Plasmid contains the gene for anti-CRISPR protein AcrF3 (gene product 35 from bacteriophage JBD5).

Depositor

Blake Wiedenheft

Article

Rollins et al Proc Natl Acad Sci U S A. 2017 Apr 24. p

Insert

AcrF3

Tags

6xHis-TEV

Available Since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-FKBP-mTagBFP2-MCS

Plasmid

#178864

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of FKBP-mTagBFP2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

FKBP-mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAG552-MAP4-redFAST

Plasmid

#160367

Purpose

Expresses MAP4-redFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

MAP4-redFAST

Expression

Mammalian

Available Since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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peSpCas9-J23109

Plasmid

#113152

Purpose

Plasmid constitutively expressing eSpCas9 protein

Depositor

Chong Zhang

Article

Guo et al Nucleic Acids Res. 2018 Aug 21;46(14):70

Insert

eSpCas9

Expression

Bacterial

Mutation

K810A & K1003A & R1060A

Available Since

Dec. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pBT362

Plasmid

#127844

Purpose

clone BE4max-NG CBEs by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

BE4max-NG without deaminase

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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FUp95bGW (B6)

Plasmid

#74015

Purpose

lentiviral expression of shRNA resistant Psd95-beta-EGFP fusion

Depositor

Robert Malenka, Oliver Schluter, Weifeng Xu

Article

Schluter et al Neuron. 2006 Jul 6;51(1):99-111.

Insert

Psd95 beta isoform

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

shRNA resistant Psd95

Promoter

hUbC

Available Since

March 31, 2016

Availability

Academic Institutions and Nonprofits only
pDest472 SHP-1 S591D

Plasmid

#21806

Depositor

Stephen Shaw

Article

Liu et al J Leukoc Biol. 2007 Sep . 82(3):742-51.

Insert

SHP-1 (PTPN6 Human)

Tags

GFP

Expression

Mammalian

Mutation

changed Serine 591 to Aspartic Acid

Available Since

Aug. 11, 2009

Availability

Academic Institutions and Nonprofits only
pBT100.136c

Plasmid

#122603

Purpose

clone FERNY phage by SapI Golden Gate

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

FERNY

Expression

Bacterial

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pMX1433

Plasmid

#127857

Purpose

Expresses EGFP-deltaN-term-MCAK in mammalian cells

Depositor

Linda Wordeman

Article

Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

Insert

deltaN-term-MCAK

Tags

EGFP

Expression

Mammalian

Mutation

deletion of first 19 codons of MCAK

Promoter

CMV

Available Since

July 5, 2019

Availability

Academic Institutions and Nonprofits only

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