We narrowed to 62,913 results for: cloning
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Plasmid#110866PurposeLentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)DepositorInsertCas9-HF1RA
UseLentiviralTagsFLAGMutationR661A, Q695A, Q926A, and NLS sequence at the N-te…PromoterEF1sAvailable sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pRK2-AraE
Plasmid#110141PurposeConstitutive promoter expressing araE on RK2 originDepositorInsertaraE
ExpressionBacterialAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCK306
Plasmid#110544PurposerhaBAD, a rhamnose-inducible promoter, YFP, an E. coli-Synechocystis shuttle vector, chromosomal-integration sites. Allows precise & sustained gene expression in cyanobacteria.DepositorInsertsPrhaBAD
yfp
rhaS
ExpressionBacterialPromoterN/A, PrhaBAD, and kanR promoter (upstream of kanR…Available sinceJune 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
LentiRCas9-CUG
Plasmid#104183PurposeLentiviral transfer vector that carries U6-driven sgRNA targeting CUG repeats using a modified scaffold (Chen et al. Cell 2013) and CMV-driven PIN-dCas9. Derived from LentiCRISPR v2 (Zhang lab)DepositorInsertU6-CUGsgRNA, EFS-PIN-dCas9
UseCRISPR and LentiviralAvailable sinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEJS887_pTetR-P2A-BFPnls/sgAlpha
Plasmid#108651PurposeAlpha satellite repeats targeting Spy sgRNA under U6 promoterDepositorInsertsAlpha satellite repeats targeting sgRNA
TetR-P2A-BFP
UseCRISPR and LentiviralTagsNoExpressionMammalianPromoterU6 and hPGKAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEGFP‐RNASEH2C
Plasmid#108698PurposeFor expression of N-terminally eGFP-tagged human RNASEH2C in mammalian cellsDepositorAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn1-tdTomatof
Plasmid#104060PurposeAlso known as hSyn1-tdTomato-f. AAV expression of Farnylsated tdtomato for tracingDepositorInserttdTomato
UseAAVPromoterhSynAvailable sinceApril 18, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSMART-SOS-GFPuv
Plasmid#102283PurposeFluorescent sensor of E. coli SOS responseDepositorInsertcda-GFPuv
ExpressionBacterialPromotercda SOS promoterAvailable sinceApril 12, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
ecAT3.10
Plasmid#107215PurposeMammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.DepositorInsertecAT3.10
ExpressionMammalianPromoterCMVAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pmec-4::Chrimson::SL2::mCherry::unc-54
Plasmid#107745PurposeExpress Chrimson and mCherry in mechanosensory neurons.DepositorInsertsChrimson
mCherry
ExpressionInsect and WormPromotermec-4Available sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a_T5-ARG1
Plasmid#106476PurposeExpresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli Nissle 1917DepositorInsertARG1
UseSynthetic BiologyExpressionBacterialPromoterT5Available sinceMarch 23, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-SnoopTagJr-MBP
Plasmid#105628PurposePlasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigaseDepositorInsertSnoopTagJr-MBP
ExpressionBacterialPromoterT7Available sinceMarch 16, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pKK-CyOFP1-TEV
Plasmid#105782PurposeExpression of your protein of interest in fusion with orange fluorescent protein at the N-terminus (cleavable by TEV) (PMID: 27240196). CyOFP1 is useful for multi-channel imaging.DepositorTypeEmpty backboneUseFlp-in competentTagsCyOFP1-TEVExpressionMammalianAvailable sinceFeb. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-N2cG
Plasmid#100811Purposeexpress rabies virus CVS N2c strain glycoprotein gene (N2cG)DepositorInsertrabies virus CVS N2c strain glycoprotein gene
ExpressionMammalianPromoterCAGAvailable sinceFeb. 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MMW948: CAG-DmrC-NLS-3xFLAG-VPR
Plasmid#104565PurposeMammalian expression vector for DmrC fused to VPR activatorDepositorInsertDmrC-VPR
Tags3x FLAG and NLSExpressionMammalianPromoterCAGAvailable sinceJan. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEF5-FRT-V5-DEST-GFP-Kif26b
Plasmid#102862PurposeExpresses GFP-Kif26b in mammalian cellsDepositorAvailable sinceDec. 8, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAD-LyseR (in lacZ deficient strain)
Plasmid#99245PurposeFor autolysate production in lacZ deficient (lac operon deletion) E. coli BL21-Gold-dLac (DE3) strain: constitutively expresses phage lambda endolysin (gene R)DepositorAvailable sinceOct. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hsyn_NES-his-CaMPARI2-F391W-G395D-WPRE-SV40
Plasmid#101063PurposeAAV vector expressing CaMPARI2_F391W-G395D (Kd = 530nM), a photoconvertible fluorescent protein-based calcium integrator, in neuronsDepositorInsertCaMPARI2_F391W-G395D
UseAAVTagsFLAG-HA-myc and NES_hisPromoterhsyn (synapsin-1)Available sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HNF1B_pLX307
Plasmid#98342PurposeLentiviral expression of HNF1BDepositorAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
IQGAP1_pLX307
Plasmid#98346PurposeLentiviral expression of IQGAP1DepositorAvailable sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only