We narrowed to 11,231 results for: SHA

1. HLH Clock Pas 1-401 VenN L113E

   Plasmid

   #47356

   Purpose

   Clock fragment mutatation tagged with VenN used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, L113E

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

2. HLH Clock Pas 1-401 VenN F122D

   Plasmid

   #47357

   Purpose

   Clock fragment mutatation tagged with VenN used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, F122D

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

3. HLH Clock Pas 1-401 VenN W284A

   Plasmid

   #47358

   Purpose

   Clock fragment mutatation tagged with VenN used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, W284A

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. HLH Clock Pas 1-401 VenN V315R

   Plasmid

   #47359

   Purpose

   Clock fragment mutatation tagged with VenN used for BiFC

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, V315R

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

5. mutpRL-TK 1x perfect to bulged siRNA

   Plasmid

   #40765

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to bulged target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. mutpRL-TK 1x perfect to seed siRNA

   Plasmid

   #40766

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to seed target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. mutpRL-TK 1x perfect to seed plus 13-16 siRNA

   Plasmid

   #40767

   Purpose

   To evaluate the efficacy of silencing and the extent of cooperativity directed by a small silencing RNA bound at one or multiple sites on an mRNA

   Depositor

   Phillip Zamore

   Article

   Broderick et al RNA. 2011 Oct;17(10):1858-69. Epub 2011

   Insert

   CXCR4 small RNA perfect to seed plus 13-16 target site
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mutation

   mutated from TAG to CTC at nt 387–389 of the Reni…

   Promoter

   TK

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

8. HLH Clock Pas 1-401 VenN L57E

   Plasmid

   #47354

   Purpose

   Clock fragment mutatation tagged with VenN

   Depositor

   Joseph Takahashi

   Article

   Huang et al Science. 2012 Jul 13;337(6091):189-94. d

   Insert

   Clock (Clock Mouse)
   

   Use

   Tags

   Venus aa 1-155

   Expression

   Mammalian

   Mutation

   aa 1-401, L57E

   Promoter

   CMV

   Available since

   Sept. 4, 2013

   Availability

   Academic Institutions and Nonprofits only

9. integrin beta6 777T pBS

   Plasmid

   #13581

   Depositor

   Dean Sheppard

   Article

   Morris et al Nature. 2003 Mar 13. 422(6928):169-73.

   Insert

   integrin beta 6 777T (ITGB6 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   777T. Lacks last 11 amino acids of cytoplasmic do…

   Promoter

   Available since

   Jan. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. dCas9-KRAB

    Plasmid

    #110820

    Purpose

    Conventional dCas9 repressor-dCas9-KRAB

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-mGold2s-Calnexin-N-14

    Plasmid

    #231768

    Purpose

    Visualization of ER in mammalian cells using mGold2s

    Depositor

    Francois St-Pierre

    Article

    Lee et al Nat Commun. 2025 Apr 4;16(1):3241. doi:

    Insert

    mGold2s-Calnexin-N-17 (CANX Human)
    

    Use

    Tags

    mGold2s

    Expression

    Mammalian

    Mutation

    I474N

    Promoter

    CMV

    Available since

    Feb. 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-hsyn-ASAP5-WPRE

    Plasmid

    #225709

    Purpose

    Pan-neuronal, pan-membrane expression of the genetically encoded voltage indicator ASAP5; can be used for dendritic voltage imaging

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. H3K9me3 biosensor

    Plasmid

    #120802

    Purpose

    FRET biosensor. To monitor histone H3 lysine 9 tri-methylation in mammalian cells by FRET

    Depositor

    Yingxiao Wang

    Article

    Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

    Insert

    Truncated YPet-HP1-EV linker-ECFP-mouse histone H3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pACgp67B-Her2

    Plasmid

    #10794

    Depositor

    Judy Lieberman

    Article

    Song et al Nat Biotechnol. 2005 Jun . 23(6):709-17.

    Insert

    ErbB2 ScFv (ERBB2 Human)
    

    Use

    Tags

    6His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    April 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pcDNA3.1-Flag-mRIPK1-S321E

    Plasmid

    #115340

    Purpose

    Mammalian expression of Flag-mRIPK1-S321E

    Depositor

    Junying Yuan

    Article

    Geng et al Nat Commun. 2017 Aug 25;8(1):359. doi: 1

    Insert

    RIPK1 (Ripk1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S321E

    Promoter

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFB2E

    Plasmid

    #195288

    Purpose

    Bicistronic HSV-TK-EGFP fusion and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    HSV-TK EGFP 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFB2D

    Plasmid

    #195286

    Purpose

    Bicistronic DTR-EGFP fusion and Puromycin resistance for positive/negative selection

    Depositor

    Scott Lowe

    Article

    Barriga et al Nat Cancer. 2022 Nov;3(11):1367-1385. do

    Insert

    DTR EGFP 2A Puro
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    Jan. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3-Myc-AIM2

    Plasmid

    #73958

    Purpose

    Express c-Myc-tagged human AIM2 in mammalian cells

    Depositor

    Christian Stehlik

    Article

    Khare et al Nat Immunol. 2014 Apr;15(4):343-53. doi:

    Insert

    absent in melanoma 2 (AIM2) (AIM2 Human)
    

    Use

    Tags

    c-myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV iE

    Available since

    April 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHAGE2-EF1a-E1MLS-2×FLAG-T4Lig-IRES-tdTomato

    Plasmid

    #234952

    Purpose

    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAAV-hsyn-ASAP5-Kv2.1-WPRE

    Plasmid

    #225707

    Purpose

    Pan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5

    Depositor

    Michael Lin

    Article

    Hao et al Neuron. 2024 Sep 17:S0896-6273(24)00643-

    Insert

    ASAP5-Kv2.1
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    human synapsin

    Available since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pX330-PITCh-LMNB1

    Plasmid

    #207770

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of LMNB1 for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of LMNB1 (LMNB1 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pZac2.1-GfaABC1D-mCherry-hPMCA2w/b

    Plasmid

    #111568

    Purpose

    Expresses a plasma membrane calcium pump hPMCA2w/b

    Depositor

    Baljit Khakh

    Article

    Yu et al Neuron. 2018 Aug 27. pii: S0896-6273(18)

    Has Service

    AAV5

    Insert

    Plasma membrane calcium pump 2, variant w/b (ATP2B2 Human)
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    Promoter

    Available since

    Sept. 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pSLIK 3xFLAG-MAVS hygro

    Plasmid

    #158634

    Purpose

    Lentiviral expression vector for an inducible 3xFLAG-tagged MAVS construct

    Depositor

    Kevin Janes

    Article

    Lopacinski et al Cell Syst. 2021 Apr 21;12(4):304-323.e13

    Insert

    MAVS (MAVS Human)
    

    Use

    Lentiviral

    Tags

    3XFLAG

    Expression

    Mutation

    Promoter

    Available since

    Sept. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. CNOT3

    Plasmid

    #155467

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    CNOT3 (CNOT3 Human)
    

    Use

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available since

    Nov. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

    Plasmid

    #234950

    Purpose

    Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pHAGE2-EF1a-E1MLS-2xFLAG-LigD-IRES-tdTomato

    Plasmid

    #234951

    Purpose

    Human codon optimized LigD (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    Human codon optimized LigD with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. ppyCAG_RNaseH1_D210N

    Plasmid

    #111904

    Purpose

    Express D210N mutated RNASEH1 which abolishes the catalytic activity of RNASEH1

    Depositor

    Xiang-Dong Fu

    Article

    Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

    Insert

    RNASEH1 (RNASEH1 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Changed D 210 to N

    Promoter

    Available since

    July 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pX330-PITCh-ACTB

    Plasmid

    #207748

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the N-terminus of ACTB for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting N-terminus of ACTB (ACTB Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pLX304-MARK3

    Plasmid

    #107235

    Purpose

    Lentiviral gene expression vector for phospho-MARK3 expression

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Insert

    MARK3
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. dCas9-KRAB-MeCP2

    Plasmid

    #110821

    Purpose

    Improved dCas9 repressor-dCas9-KRAB-MeCP2

    Depositor

    Alejandro Chavez, George Church

    Article

    Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

    Insert

    dCas9-KRAB-MeCP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

    Promoter

    CMV

    Available since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge