We narrowed to 7,470 results for: addgene/1000

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FENEF-Sensor

Plasmid

#208930

Purpose

in vitro p38-Sensor for luminescent detection of p38-activity

Depositor

Attila Reményi

Article

Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854.

Insert

FENEF sequence from human ATF2 with phospho site (ATF2 Human)

Tags

HISx6, MBP, and Smbit

Expression

Bacterial

Promoter

T7

Available Since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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cacna2d1 R241A HA pMT2

Plasmid

#206111

Purpose

expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with an R421A mutation that disrupts Gabapentin binding

Depositor

Annette Dolphin

Article

Cassidy et al Proc Natl Acad Sci U S A. 2014 Jun 17;11

Insert

cacna2d1 (Cacna2d1 Rat)

Expression

Mammalian

Mutation

R241A

Promoter

Ad MLP/TPL/SV40

Available Since

Nov. 28, 2023

Availability

Academic Institutions and Nonprofits only

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B52_puro_gRNA_3418711_KCNB2

Plasmid

#197558

Purpose

gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)

Depositor

Darius Bägli

Insert

upstream KCNB2 promoter gRNA (Kcnb2 Rat)

Use

Grna with puromycin selection

Tags

puromycin resistance cassette under CMV promotion

Expression

Mammalian

Promoter

U6

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO2-K663AL697Y_AC

Plasmid

#148427

Purpose

Mammalian Expression of HsAGO2-K663AL697Y

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2-K663AL697Y (AGO2 Human)

Expression

Mammalian

Mutation

three silent mutations and the sequence differs i…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsAGO2-P593GA623FK663AL697Y_AC

Plasmid

#148428

Purpose

Mammalian Expression of HsAGO2-P593GA623FK663AL697Y

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2-P593GA623FK663AL697Y (AGO2 Human)

Expression

Mammalian

Mutation

three silent mutations and the sequence differs i…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-TAPtagN-PrP-SBP-HsAGO2_AC

Plasmid

#148440

Purpose

Mammalian Expression of HsAGO2

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsAGO2 (AGO2 Human)

Expression

Mammalian

Mutation

one silent mutation C840Tand sequence differs in …

Available Since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-SynaptoZip

Plasmid

#177318

Purpose

AAV expression of the synaptic activity-marker SynaptoZip fused to mCherry driven by human Synapsin promoter

Depositor

Antonio Malgaroli

Article

Lamanna et al Sci Rep. 2022 Jun 30;12(1):11055. doi: 1

Insert

mCherry-SynaptoZip (Vamp2 Rat)

Use

AAV

Tags

Myc and mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pJET-22-DExCon-mNeonGreen-R11a

Plasmid

#179897

Purpose

Donor with homologous arms for Rab11a to knock in DExCon-mNeonGreen module

Depositor

Patrick Caswell

Article

Gemperle et al Elife. 2022 Jun 16;11. pii: 76651. doi:

Insert

RAB11A, member RAS oncogene family (RAB11A Human)

Use

CRISPR; Donor for homologous based knock in

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Promoter

TRE3GS

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-3exo-Tetra-com-CLCN5-sp-g1

Plasmid

#176236

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-5Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176237

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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pspCas9-Exo-tetra-com-hCLCN5-sp-g1

Plasmid

#176238

Purpose

Plasmid for expressing a fusion protein of spCas9 and the 5’ and 3’ exonuclease domain of POLI, and sgRNA targeting human CLCN5 gene.

Depositor

Baisong Lu

Article

Yoo et al Nucleic Acids Res. 2022 Mar 22. pii: 655

Insert

spCas9 and polymerase exo domain fusion (CLCN5 Human)

Expression

Bacterial

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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PWPXLD-EGFR-P667A

Plasmid

#133750

Purpose

EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

Depositor

Chay Kuo

Article

Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

Insert

Mutant P667A EGFR-HA (EGFR Human)

Use

Lentiviral

Tags

HA tag

Expression

Mammalian

Mutation

proline 667 converted to alanine (Please see depo…

Promoter

EF1-alpha

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-H3

Plasmid

#124435

Purpose

Chimeric ETS domain: H3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis Tag

Expression

Bacterial

Mutation

Residues 225 to 239 replaced with corresponding s…

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-Loop

Plasmid

#124551

Purpose

Chimeric ETS domain: Loop of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 216 to 223 replaced with corresponding s…

Available Since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pET28B-PU.1-ETS-S3

Plasmid

#124550

Purpose

Chimeric ETS domain: S3 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Ets1, Spi1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 242 to 246 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pET28b-PU.1-ETS-H2

Plasmid

#123358

Purpose

Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold

Depositor

Gregory Poon

Article

Albrecht et al Nucleic Acids Res. 2018 Nov 16;46(20):10

Insert

PU.1 ETS domain (residues 167-272) (Spi1, Ets1 Mouse)

Tags

6xHis tag

Expression

Bacterial

Mutation

Residues 207 to 215 replaced with corresponding s…

Available Since

April 29, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-Flag-CSNK1A1 G40N-P2A-Blast

Plasmid

#123321

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Blast casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available Since

March 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-CD4-YEML-myc

Plasmid

#58538

Purpose

Expresses human CD4 with the IFITM3 endocytosis motif (YEML) and a C-terminal myc tag in mammalian cells.

Depositor

Jacob Yount

Article

Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

Insert

CD4 (CD4 Human)

Tags

IFITM3 endocytosis Tyrosine-Glutamate-Methionine-…

Expression

Mammalian

Mutation

Added C-terminal Tyrosine-Glutamate-Methionine-Le…

Available Since

Sept. 16, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-M100/125/147L-siResist

Plasmid

#49856

Purpose

Encodes human connexin 43 with M100, M125, and M147 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100, Methionine 125, and Methionine 147…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3.2-Cx43-M100/125L-siResist

Plasmid

#49855

Purpose

Encodes human connexin 43 with M100 and M125 mutated to L and wobble mutations conferring resistance against an siRNA

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Expression

Mammalian

Mutation

Methionine100 and Methionine 125 mutated to Leuci…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only