We narrowed to 6,558 results for: sas;

1. pIN2 (gpdA-nTerm-mGL-ptrA)

   Plasmid

   #188736

   Purpose

   Expresses mGreenlantern for N-terminal tagging

   Depositor

   Michael Bromley

   Article

   Storer et al Antimicrob Agents Chemother. 2024 Nov 6;

   Insert

   gdpA-mGreenlantern PtrA
   

   Use

   Filamentous fungal expression

   Tags

   mGreenlantern

   Available Since

   Nov. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS_4xMma PylT_FLAG-Mma PylRS AF

   Plasmid

   #140023

   Purpose

   Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Y271A, Y349F

   Promoter

   EF1

   Available Since

   May 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAS_4xMma PylT sfGPF 150TAG

   Plasmid

   #154766

   Purpose

   plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

   Insert

   sfGFP
   

   Expression

   Mammalian

   Mutation

   150TAG in GFP reporter, U25C in PylT

   Promoter

   EF1

   Available Since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK5-FLAG-Sestrin1 isoform1

   Plasmid

   #72594

   Purpose

   overexpression, codon optimized for mammalian expression

   Depositor

   David Sabatini

   Article

   Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

   Insert

   SESN1 (SESN1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon optimized for mammalian expression, see dep…

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

5. OPRM1-Tango

   Plasmid

   #66464

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   OPRM1 (OPRM1 Human)
   

   Tags

   FLAG and tTA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. EB3N-VVDfast-Halo-iLID

   Plasmid

   #190166

   Purpose

   Expresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.

   Depositor

   Anna Akhmanova

   Article

   Meiring et al Curr Biol. 2022 Nov 7;32(21):4660-4674.e

   Insert

   EB3N-VVDfast-Halo-iLID
   

   Tags

   -VVDfast-mClover3-iLID

   Expression

   Mammalian

   Mutation

   1-189 aa from EB3

   Available Since

   Oct. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. SSTR4-Tango

   Plasmid

   #66505

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   SSTR4 (SSTR4 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. PLJR962

   Plasmid

   #115162

   Purpose

   Sth1 dCas9 TetR and KanR L5 Int attP for M. smegmatis

   Depositor

   Sarah Fortune

   Article

   Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

   Inserts

   Sth1 sgRNA scaffold
   Sth1 dCas9
   TetR
   L5 attP
   L5 Int
   KanR
   

   Use

   Unspecified

   Available Since

   Sept. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. NPY5R-Tango

   Plasmid

   #66456

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   NPY5R (NPY5R Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

10. NPY2R-Tango

    Plasmid

    #66454

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    NPY2R (NPY2R Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pAAV-Ucp1-empty-miR122

    Plasmid

    #231695

    Purpose

    Empty control vector for pAAV-Ucp1-Cre-miR122

    Depositor

    Joerg Heeren

    Article

    Behrens et al Mol Metab. 2024 Oct;88:101999. doi: 10.1

    Type

    Empty backbone

    Use

    AAV

    Promoter

    rat Ucp1 enhancer-promoter

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. PLJR965

    Plasmid

    #115163

    Purpose

    Sth1 dCas9 TetR and KanR L5 Int attP for M. tuberculosis

    Depositor

    Sarah Fortune

    Article

    Rock et al Nat Microbiol. 2017 Feb 6;2:16274. doi:

    Inserts

    Sth1 sgRNA scaffold
    Sth1 dCas9
    TetR (deleted TetON) MtbCO
    L5 attP
    L5 Int
    KanR
    

    Use

    Unspecified

    Available Since

    Sept. 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. SSTR2-Tango

    Plasmid

    #66503

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    SSTR2 (SSTR2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET15b His-p97

    Plasmid

    #169020

    Purpose

    Expresses His-p97 in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. TSHR-Tango

    Plasmid

    #66518

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    TSHR (TSHR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL3617 (pCRISPR(BbsI)_Pse)

    Plasmid

    #200902

    Purpose

    Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

    Depositor

    Samuel H. Sternberg

    Article

    Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

    Insert

    PseCRISPR (entry)
    

    Expression

    Mammalian

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. SSTR5-Tango

    Plasmid

    #66506

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    SSTR5 (SSTR5 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

18. pAD-KPS12-Pman

    Plasmid

    #133832

    Purpose

    Fluorescent protein mKate(KPS12) variant for optimized expression in Bacillus mycoides; under control of mannose inducible promoter.

    Depositor

    Oscar Kuipers

    Article

    Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

    Insert

    mKate(KPS12)
    

    Use

    E.coli/bacillus spp. shuttle vector

    Expression

    Bacterial

    Promoter

    Pman

    Available Since

    Nov. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. MRGPRX2-Tango

    Plasmid

    #66440

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    MRGPRX2 (MRGPRX2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

20. pPBbsr2-4031NES

    Plasmid

    #105241

    Purpose

    A Förster resonance energy transfer (FRET) biosensor for AMPK activity with cyan and yellow fluorescent protein.

    Depositor

    Michiyuki Matsuda

    Article

    Konagaya et al Cell Rep. 2017 Nov 28;21(9):2628-2638. d

    Insert

    AMPK-EV
    

    Expression

    Mammalian

    Promoter

    CAGGS

    Available Since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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