We narrowed to 24,925 results for: crispr
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Plasmid#118638PurposeTransient transfection; Expresses dCasRx-RBM38; CAGGS promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdCasRx-RBM38
UseCRISPRExpressionMammalianPromoterCAGGSAvailable sinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pV1025
Plasmid#111424PurposeExpression of Candida albicans codon-optimized Cas9 (CaCas9) - integrates at ENO1 (Part of Duet system)DepositorPublicationInsertCaCas9
UseCRISPRExpressionYeastAvailable sinceOct. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSET-dCas9-actII-4-NT-S1
Plasmid#110185PurposeRepression of gene expression in Streptomyces by CRISPRiDepositorInsertsdCas9
sgRNA
UseCRISPR; IExpressionBacterialMutationD10A, H840APromoterJ23119 and ermE*pAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAF-HdrB3
Plasmid#184908Purposecarrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferriduransDepositorInsertCas9-sgRNA-homology arms
UseCRISPR; Broad-host-rangeExpressionBacterialAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBbS8c-ddcpf1-Δ
Plasmid#153039PurposeFor CRISPRi. Arabinose-inducible ddcpf1 gene with CRISPR array lacking a gene-specific spacer.DepositorInsertAscpf1
ExpressionBacterialMutationE993AAvailable sinceMay 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDHt/sk-CE
Plasmid#92128PurposeExpression of codon-optimized Cas9-eGFP fusion protein with Pcbh1 promoter in Trichoderma reeseiDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCas9-eGFP
UseCRISPR; Trichoderma reesei expressionTagsEGFPPromoterPcbh2Available sinceNov. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT001
Plasmid#182711PurposeaTc inducible dCasRxDepositorInsertdCasRx
UseCRISPRTags2x-FLAGExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
GuideVector_BbsI
Plasmid#110692PurposeFor rapid cloning and expression of sgRNAs in mammalian cells. Contains copGFP for easy FACS sorting.DepositorTypeEmpty backboneUseCRISPRExpressionMammalianAvailable sinceMay 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
pVC297 VEGF Site#1
Plasmid#47505Purposehuman gRNA expression vector targeting VEGFDepositorAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GG-EBNA-OSK2M2L1-PP
Plasmid#102902PurposeEBNA episome plasmid for U6 promoter-driven expression of 10 gRNAs targeting OCT4, MYC, KLF4, SOX2 and LIN28A promoters. Includes PGK-puro selection cassette.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOSK2M2L1-gRNAs-PGK-puro
UseCRISPRExpressionMammalianPromoterU6Available sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CAG-SaCas9-WPRE
Plasmid#89996PurposeExpresses high levels of WT SaCas9 for genome editingDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSaCas9 (NEWENTRY Staphylococcus aureus)
Tags3xHA, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCAGAvailable sinceSept. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pY036_ATP1A1_G3_Array
Plasmid#86619PurposeVector for expression of a CRISPR/AsCpf1 array containing the ATP1A1 G3 guide in combination with a user-specified guide. Cloning of oligos for the second guide using BbsI sites. PY036-like plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertATP1A1 G3 crRNA + user-specified crRNA + AsCpf1-3xHA
UseCRISPR; Co-selection via nhej using ouabainTags3xHAExpressionMammalianPromoterCBhAvailable sinceMarch 16, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FASTHDR-Cterm-mRuby3-Zeo
Plasmid#167206PurposePlasmid to clone recombination arms to create homologous recombination donor vector for C-terminal gene tagging with mRuby3 and selection with ZeocinDepositorTypeEmpty backboneUseCRISPRTagsmRuby3ExpressionMammalianAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYL-kasOp-FnCas12a2
Plasmid#177874PurposeExpresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStreptomyces codon optimized FnCas12a
UseCRISPRPromoterkasOp*Available sinceJan. 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAC92-pmax-dCas9VP96
Plasmid#48224PurposedCas9VP96 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP96 activation domain
UseCRISPRTagsHA Tag and VP96ExpressionMammalianMutationD10A;H840APromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFnCas12a_NT
Plasmid#169838PurposeExpresses FnCas12a and guide RNA in BacteriaDepositorInsertFnCas12a
UseCRISPRExpressionBacterialPromoterpXynAAvailable sinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9GFP-IRES-Neo
Plasmid#102907PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of dCas9-GFP followed by IRES-Neomycin selection cassette.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertDDdCas9GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable sinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCas9
Plasmid#162606PurposePre-express Cas9 in yeastDepositorInsertTef1-Cas9
ExpressionYeastAvailable sinceJune 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
BB3cK_pGAP_23*_pPFK300_Cas9
Plasmid#104910PurposehCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on G418DepositorInsertsHH - FS23 linker - HDV
human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
UseCRISPRExpressionYeastAvailable sinceApril 8, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by