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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,439 results for: eng

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Showing: 5061 - 5080 of 14439 results

  1. AKARdual

    Plasmid
    #99869
    Purpose
    Measure PKA activity by conformational change after PKA substrate phosphorylation and recognition of this phosphorylated substrate
    Depositor
    Marc Tramier
    Publication
    Demeautis et al Sci Rep. 2017 Jan 20;7:41026. doi: 10.10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AKAR4
    Tags
    LSSmOrange and mKate2
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 1, 2017
    Availability
    Academic Institutions and Nonprofits only
    99869-plasmid-map-sequence-id-192827

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  3. PX383 Lactobacillus johnsonii Cas9

    Plasmid
    #68320
    Purpose
    Lactobacillus johnsonii Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LjCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68320-plasmid-map-sequence-id-119535

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  4. dTomato_LD 0_CS M_tTA-BFP

    Plasmid
    #58875
    Purpose
    MESA target chain with dTomato ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusion
    Depositor
    Joshua Leonard
    Publication
    Daringer et al ACS Synth Biol. 2014 Mar 11.
    Insert
    MESA target chain with dTomato ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
    Use
    AAV
    Tags
    EBFP
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58875-plasmid-map-sequence-id-94236

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  8. pU6_B2M_sgRNA(PP7)

    Plasmid
    #232438
    Purpose
    sgRNA to facilitate a splice donor site disruption via adenine base editing in the B2M locus, contains a PP7 aptamer in the scaffold tetraloop
    Depositor
    Gil Westmeyer
    Publication
    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-tagged gRNA for B2M disruption via splice donor consensus disruption via ABE8e or Cas9 driven by human U6 promoter
    Use
    CRISPR
    Promoter
    U6
    Available since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    232438-plasmid-map-sequence-id-462755

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  9. pPB9v1

    Plasmid
    #210028
    Purpose
    Contains Cas9 gRNA cloning site, inducible Cas9, rtTA-T2A-puro
    Depositor
    Michael Talkowski
    Publication
    Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    Cas9
    rtta-T2A-puro
    Use
    CRISPR
    Tags
    SV40 NLS and nucleoplasmin NLS
    Expression
    Mammalian
    Promoter
    EF-1α and TRE3G
    Available since
    Feb. 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    210028-plasmid-map-sequence-id-413997

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  18. pUC-STITCH

    Plasmid
    #129535
    Purpose
    STITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.
    Depositor
    Hideyuki Okano
    Publication
    Tsujimura et al Elife. 2020 May 5;9. pii: 47980. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    STITCH
    Use
    CRISPR; Donor template
    Available since
    Dec. 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    129535-plasmid-map-sequence-id-265185

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  20. pCAG-AaCas12b(R911A)-2AeGFP

    Plasmid
    #121961
    Purpose
    Mammalian expression, Transcription regulation, deactivated AaCas12b
    Depositor
    Wei Li
    Publication
    Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Catalytically deactivated AaCas12b
    Tags
    3xHA tag
    Expression
    Mammalian
    Mutation
    R911A
    Available since
    Feb. 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    121961-plasmid-map-sequence-id-232188

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Showing: 5061 - 5080 of 14439 results