159,557 results

1. Puro-iNgn3

   Plasmid

   #75337

   Purpose

   doxycycline inducible Ngn3 over-expression

   Depositor

   Danwei Huangfu

   Article

   Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

   Insert

   neurogenin 3 (Neurog3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE-tight

   Available since

   May 24, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. cyto-iATPSnFR1.0

   Plasmid

   #102550

   Purpose

   cytoplasmic ATP sensor for mammalian cells

   Depositor

   Baljit Khakh

   Article

   Lobas et al Nat Commun. 2019 Feb 12;10(1):711. doi:

   Insert

   iATPSnFR1.0
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. MLRV

   Plasmid

   #118069

   Purpose

   Multi-luciferase reporter vector including transcriptional reporters for NF-kb, TGF-b, c-Myc, p53 and MAPK/JNK transcriptional reporters

   Depositor

   Koen Venken

   Article

   Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

   Inserts

   TB:5xNF-κβ:RedF:bGHpA
   TB:4xTGF-β:FLuc:bGHpA
   TB:5xE-box:Renilla:bGHpA
   TB:2xp53:NLuc:bGHpA
   TB:6xAP-1:GrRenilla:bGHpA
   CMV:ELuc:bGHpA
   

   Use

   Luciferase and Synthetic Biology; Assembly of gb2…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   2xp53::miniP, 4xTGF-B::miniP, 5xEbox::miniP, 5xNF…

   Available since

   Dec. 16, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1 hSCN5A

   Plasmid

   #145374

   Purpose

   expresses human Nav1.5 (WT) in mammalian cells

   Depositor

   Hugues Abriel, Robert Kass

   Article

   Rougier et al Am J Physiol Cell Physiol. 2005 Mar;288(

   Insert

   sodium voltage-gated channel alpha subunit 5 (SCN5A Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONR221-SLC5A2_STOP

   Plasmid

   #161407

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors. Contains a STOP codon at the end of the codon-optimized ORF sequence.

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC5A2 (SLC5A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 15, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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6. pCMV-BFP-U6-sgRNA

   Plasmid

   #196725

   Purpose

   Sortable sgRNA expression, Chen tracrRNA PMID: 24360272

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSG86-J23151-LasR

   Plasmid

   #102456

   Purpose

   Expresses LasR protein in E. coli. The expression is controlled by a constitutive promoter.

   Depositor

   Richard Murray

   Article

   Guo et al ACS Synth Biol. 2019 Mar 15;8(3):606-610

   Insert

   LasR (lasR )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23151

   Available since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCEP-Puro-TetOn-L1RP-ORF1_R261A_Halo-GFPai

   Plasmid

   #202569

   Purpose

   Expresses an endogenous-like L1RP LINE-1 element with a C-terminal HaloTag on the ORF1 protein with the R261A mutation and a GFP retrotransposition reporter (GFP-AI) in the 3' UTR

   Depositor

   Liam Holt

   Article

   Sil et al Elife. 2023 Apr 28;12:e82991. doi: 10.75

   Insert

   LINE-1
   

   Use

   Tags

   GFP-AI retrotransposition reporter and HaloTag7 o…

   Expression

   Mammalian

   Mutation

   Changed ORF1p Arginine 261 to Alanine

   Promoter

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJ23101

   Plasmid

   #70252

   Purpose

   Expresses YFP from J23101 promoter and CFP from standard J23101 promoter

   Depositor

   Jim Haseloff

   Article

   Grant et al Mol Syst Biol. 2016 Jan 25;12(1):849.

   Insert

   promoter J23101-RBS B0034-EYFP-B0015
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   April 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCXLE-mCherry-t2a-SOX2-p2a-KLF4

    Plasmid

    #193296

    Purpose

    episomal mCherry-hSK (expressing mCherry + human SOX2 + KLF4)

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry-t2a-SOX2-p2a-KLF4 (SOX2, KLF4 Human, Discosoma)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-CAG-H2B-GFP

    Plasmid

    #116869

    Purpose

    Nuclear localized GFP

    Depositor

    Loren Looger

    Article

    Economo et al Nature. 2018 Nov;563(7729):79-84. doi: 1

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pD649-HAsp-SEMA3E-COMP5AP-AviTag-9xHis

    Plasmid

    #157534

    Purpose

    Mammalian expression of secreted protein fused to COMP5AP-AviTag-9xHis.

    Depositor

    Chris Garcia

    Article

    Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

    Insert

    SEMA3E (SEMA3E Human)
    

    Use

    Tags

    COMP5AP-AviTag-9xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV/SP6

    Available since

    March 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. M4754 KanMX::HIS3 Disruptor Converter

    Plasmid

    #51684

    Purpose

    Yeast marker swap plasmid for converting KanMX3 to HIS3

    Depositor

    David Stillman

    Article

    Voth et al Yeast. 2003 Aug;20(11):985-93.

    Insert

    HIS3 (HIS3 Budding Yeast)
    

    Use

    Yeast marker swap

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMXs-IP GFP-WIPI-1

    Plasmid

    #38272

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Autophagy. 2010 Aug;6(6):764-76.

    Insert

    WD repeat domain phosphoinositide-interacting protein 1 (WIPI1 Human)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 16, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti_CMV: Flag-Akaluc_SV40:PuroR

    Plasmid

    #183048

    Purpose

    Expresses FLAG-tagged AkaLuc (Far-Red Firefly luciferase) in mammalian cells.

    Depositor

    Antonio Amelio

    Article

    Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

    Insert

    AkaLuc
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUltra

    Plasmid

    #24129

    Purpose

    3rd generation Lentiviral vector for bi-cistronic expression of EGFP and the gene of interest

    Depositor

    Malcolm Moore

    Article

    Lou et al PLoS One. 2012;7(3):e33093. doi: 10.1371

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWS5399-CRISPRai-ZeoR

    Plasmid

    #182838

    Purpose

    Saccharomyces cerevisiae inducible CRISPRai vector - ZeoR marker

    Depositor

    Rodrigo Ledesma-Amaro

    Article

    Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDEST53-LRRK2-G2019S

    Plasmid

    #25045

    Depositor

    Mark Cookson

    Article

    Greggio et al Neurobiol Dis. 2006 Aug . 23(2):329-41.

    Insert

    LRRK2 (LRRK2 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Glycine 2019 mutated to Serine

    Promoter

    Available since

    June 17, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.13/AUG-FFLuc-3XFLAG

    Plasmid

    #127333

    Purpose

    Express 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon

    Depositor

    Jeremy Wilusz

    Article

    Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

    Insert

    Firefly luciferase
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CV40

    Available since

    Aug. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. Orai1-jGCaMP8f

    Plasmid

    #231630

    Purpose

    Green fluorescent reporter of Orai1-associated calcium influx

    Depositor

    Michael Cahalan

    Article

    Dynes et al Proc Natl Acad Sci U S A. 2023 Sep 26;12

    Insert

    Orai1-jGCaMP8f (ORAI1 Synthetic, Human)
    

    Use

    Tags

    jGCaMP8f

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Immediate Early

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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