164,827 results

1. pLKO.1-sh-mSOX9-5

   Plasmid

   #40646

   Depositor

   Bob Weinberg

   Article

   Guo et al Cell. 2012 Mar 2;148(5):1015-28.

   Insert

   sh-mSOX9-5 (Sox9 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available Since

   Sept. 27, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pRDA_550

   Plasmid

   #203398

   Purpose

   All-in-one lentiviral expression of gRNA and EnAsCas12a

   Depositor

   John Doench

   Article

   Esmaeili Anvar et al Nat Commun. 2024 Apr 27;15(1):3577. doi:

   Insert

   Enhanced Acidaminococcus Cas12a
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBAD33-mf-lon

   Plasmid

   #21867

   Depositor

   Robert Sauer

   Article

   Gur et al Proc Natl Acad Sci U S A. 2008 Oct 21. 1

   Insert

   Lon (Mfl404 Mesoplasma florum)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   all TGA codons changed to TGG codons

   Promoter

   Available Since

   Aug. 11, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBABEpuro mTurquoise2 LC3B

   Plasmid

   #78518

   Purpose

   Fluorescent reporter for LC3B visualization

   Depositor

   David Chan

   Article

   Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

   Insert

   LC3B (MAP1LC3B Human)
   

   Use

   Retroviral

   Tags

   mTurquoise2

   Expression

   Mammalian

   Mutation

   Promoter

   retroviral

   Available Since

   Jan. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUBC-SV40NLS-Pfv-Sapphire-PURO

   Plasmid

   #203654

   Purpose

   Mammalian lentiviral vector for the expression of the Sapphire-tagged nuclear localizing 40nm nucGEMs under the UBC promoter, with a puromycin selection marker.

   Depositor

   Liam Holt

   Article

   Shu T et et al bioRxiv 2022

   Insert

   PfV
   

   Use

   Lentiviral

   Tags

   Sapphire

   Expression

   Mutation

   Promoter

   Available Since

   July 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. C1(1-29)-miniTurbo-V5_pCDNA3

   Plasmid

   #107174

   Purpose

   expresses V5-tagged miniTurbo on the mammalian ER membrane

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Promoter

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. GG-EBNA-EEA-5guides-PGK-Puro

   Plasmid

   #102898

   Purpose

   EBNA episome plasmid for U6 promoter-driven expression of 5 gRNAs targeting EEA-motif. Includes PGK-puro selection cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   EEA-5guides-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-syn-iGluSnFR4s-NGR-WPRE

   Plasmid

   #234437

   Purpose

   AAV-mediated expression of glutamate sensor with improved sensitivity and slow deactivation kinetics (4s = slow); NGR vector matches or outperforms PDGFR in all conditions tested.

   Depositor

   GENIE Project

   Article

   Aggarwal et al bioRxiv 2025.03.20.643984

   Insert

   iGluSnFR4s-NGR
   

   Use

   AAV

   Tags

   IgK chain and Myc epi tag-NGR GPI anchor

   Expression

   Mammalian

   Mutation

   Promoter

   Synapsin

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCJ190

   Plasmid

   #162667

   Purpose

   pET-21b(+) based plasmid for expresion of MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker; codon optimized for expression in E. coli K12, with C-terminal His tag.

   Depositor

   Gregg Beckham

   Article

   Knott et al Proc Natl Acad Sci U S A. 2020 Oct 13;11

   Insert

   MHETase (Genbank GAP38911.1) linked to PETase (Genbank GAP38373.1) from Ideonella sakaiensis by a 12 residue Gly/Ser linker
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   codon optimized for expression in E. coli K12

   Promoter

   T7/lac

   Available Since

   Jan. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEVOL_PylRS(WT)-tRNA

    Plasmid

    #223512

    Purpose

    PylRS (WT)/tRNA Pyl orthogonal pair for genetic code expansion that allows site-specific incorporation of noncanonical amino-acids into a POI using the Amber stop codon

    Depositor

    Edward Lemke

    Article

    Milles et al J Am Chem Soc. 2012 Mar 21;134(11):5187-

    Inserts

    Pyrrolysyl-tRNA Synthetase (2 copies) (pylS Methanosarcina mazei)
    tRNA Pyl
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD, rrnB, and glnS and proK

    Available Since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-hSyn1-FLEX-CybGam-WPRE

    Plasmid

    #207662

    Purpose

    Control for CybSEP2

    Depositor

    Sung Han

    Article

    Kim et al bioRxiv 2023.01.19.524797

    Insert

    Gamillus
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Histidine 86 and 159 to alanines, inserted gamill…

    Promoter

    hSynapsin

    Available Since

    Oct. 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-CMV-NFE2L2-Puro

    Plasmid

    #181919

    Purpose

    Lentiviral plasmid that directs the expression of NFE2L2/Nrf2 in mammalian cells.

    Depositor

    Scott Dixon

    Article

    Forcina et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

    Insert

    NFE2L2 (NFE2L2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. HaloTag-beta2-adrenergic receptor

    Plasmid

    #66994

    Purpose

    Beta-2-adrenergic receptor tagged with HaloTag on amino terminus

    Depositor

    Catherine Berlot

    Insert

    HaloTag-beta2-adrenergic receptor (ADRB2 Human)
    

    Use

    Tags

    Human Growth hormone Signal Sequence-HaloTag

    Expression

    Mammalian

    Mutation

    The signal sequence from human growth hormone was…

    Promoter

    CMV

    Available Since

    July 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDZ2FE_Pogz_1-1410

    Plasmid

    #105674

    Purpose

    Expresses Flag-tagged full-length human POGZ protein

    Depositor

    Thanos Halazonetis

    Insert

    POGZ (POGZ Human)
    

    Use

    Tags

    2X Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. TDP43-NTD

    Plasmid

    #241024

    Purpose

    Bacterial expression of TDP-43 NTD

    Depositor

    Nicolas Fawzi

    Insert

    TDP43-NTD
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    changed N81 to stop codon

    Promoter

    Available Since

    July 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. Lambda Phosphatase

    Plasmid

    #79748

    Purpose

    Intended for co-expression with human Ser/Thr kinase plasmids to enhance bacterial kinase expression.

    Depositor

    Markus Seeliger, John Chodera, Nicholas Levinson

    Article

    Albanese et al Biochemistry. 2018 Jul 13. doi: 10.1021/

    Insert

    Lambda Phosphatase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Nov. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET-28a_6H-MMLV_RT_D524N-6H

    Plasmid

    #166945

    Purpose

    Bacterial expression of M-MLV reverse transcriptase

    Depositor

    Robert Tjian

    Article

    Graham et al PLoS One. 2021 Feb 3;16(2):e0246647. doi

    Insert

    gag-pol
    

    Use

    Tags

    6X His

    Expression

    Bacterial

    Mutation

    D524N, H8Y

    Promoter

    T7

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDisplay-GRAB_eCB2.0-IRES-mCherry-CAAX

    Plasmid

    #164611

    Purpose

    Express the endocannabinoid sensor GRAB_eCB2.0 in mammalian cells

    Depositor

    Yulong Li

    Article

    Dong et al Nat Biotechnol. 2021 Nov 11. pii: 10.103

    Insert

    Endocannabinoid sensor GRAB_eCB2.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S383T

    Promoter

    CMV

    Available Since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. lentiTet-LDLR-mCherry BlastR

    Plasmid

    #186739

    Purpose

    Dox inducible LDLR coding sequence expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

    Depositor

    Richard Sherwood

    Article

    Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

    Insert

    LDLR (LDLR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. LentiGuide-Neo

    Plasmid

    #139449

    Purpose

    Lentiviral vector with gRNA scaffold and neomycin selectable marker

    Depositor

    Caroline Goujon

    Article

    Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

    Insert

    no sgRNA inserted; resistance gene: neoR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a / U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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