We narrowed to 5,300 results for: pcDNA3.1+

1. pcDNA3-mPC1_4148-eGFP

   Plasmid

   #108397

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pcDNA3-mPC1_4148-eGFP-3HA

   Plasmid

   #108396

   Purpose

   Express cytoplasmic tail mouse PC1 from amino acid 4148 to 4293

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   Pkd1 (Pkd1 Mouse)
   

   Use

   Tags

   eGFP-3HA

   Expression

   Mammalian

   Mutation

   variant ID rs13468401 missense variant R4237Q

   Promoter

   CMV

   Available since

   April 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3-hPC1_MTS-eGFP

   Plasmid

   #108402

   Purpose

   Express putative mitochondrial targeting sequence of human PC1 from amino acid 4129 to 4157

   Depositor

   Gregory Germino

   Article

   Lin et al Sci Rep. 2018 Feb 9;8(1):2743. doi: 10.1

   Insert

   PKD1 (PKD1 Mouse)
   

   Use

   Tags

   3xHA and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. TRUPATH Triple Gaq

   Plasmid

   #196058

   Purpose

   Encodes a G alpha subunit (GNAQ) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaQ-RLuc8 (GNAQ Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA-dCas9-p300 Core

   Plasmid

   #61357

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRUPATH Triple Ga13

   Plasmid

   #196062

   Purpose

   Encodes a G alpha subunit (GNA13) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlpha13-RLuc8 (GNA13 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. TRUPATH Triple GaoA

   Plasmid

   #196051

   Purpose

   Encodes a G alpha subunit (GNAO1 Isoform Alpha 1) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphaoA-RLuc8
   GGamma8-GFP2 (GNG8 Human)
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   Feb. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. TRUPATH Triple Gai1

   Plasmid

   #196048

   Purpose

   Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai1-RLuc8 (GNAI1 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA-dCas9-p300 Core (D1399Y)

   Plasmid

   #61358

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of human p300 HAT core (aa 1048-1664; inactivating mutation D1399Y) driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do

   Insert

   S.pyogenes dCas9 with c-terminal human p300 Core effector fusion (aa 1048-1664 of human p300) (EP300 S. pyogenes, Human, Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Flag, HA, and SV40 NLS

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9; D1399Y mutation i…

   Promoter

   CMV

   Available since

   April 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Kv1.2 K+ channel scFv [K14/16]

    Plasmid

    #182064

    Purpose

    Mammalian Expression of Kv1.2 K+ channel scFv. Derived from hybridoma K14/16.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Kv1.2 K+ channel (Homo sapiens) (KCNA2 Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TRUPATH Triple GaoB

    Plasmid

    #196052

    Purpose

    Encodes a G alpha subunit (GNAO1 Isoform Alpha 2) with RLuc8, a G gamma subunit (GNG8) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphaoB-RLuc8
    GGamma8-GFP2 (GNG8 Human)
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. GABA(A)R, Gamma2L/S scFv [N452/73]

    Plasmid

    #190555

    Purpose

    Mammalian Expression of GABA(A)R, Gamma2L/S scFV. Derived from hybridoma N452/73.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    GABA(A)R, Gamma2L/S (Homo sapiens) recombinant scFV (GABRG2 Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

    Plasmid

    #103871

    Purpose

    dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

    Depositor

    Feng Zhang

    Article

    Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

    Inserts

    PspCas13b
    huADAR2DD (ADARB1 Human)
    

    Use

    CRISPR

    Tags

    GS and HIV NES

    Expression

    Mammalian

    Mutation

    Changed histidne 133 to alanine and histidine 105…

    Promoter

    Available since

    Nov. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. FLAG-PRMT3

    Plasmid

    #164695

    Purpose

    Expression of PRMT3 with an N-terminal FLAG tag

    Depositor

    Cheryl Arrowsmith

    Article

    Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

    Insert

    PRMT3 (PRMT3 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CS-TRE-BclXL-Ubc-rtTA

    Plasmid

    #198189

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hBCLXL (BCL2L1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lucMAPT-GenRep

    Plasmid

    #214674

    Purpose

    MAPT Exon 10 reporter, with Exon 10 and 100bp flanking intronic regions replaced with a BamHI/EcoRI cloning site for testing different exons

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9 and Exon 11, with a BamHI EcoRI c…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. CS-TRE-BMI1-Ubc-rtTA

    Plasmid

    #198191

    Purpose

    Generation of Expandable megakaryocyte cell lines from human iPS cells

    Depositor

    Hiromitsu Nakauchi

    Article

    Nakamura et al Cell Stem Cell. 2014 Apr 3;14(4):535-48.

    Insert

    hBMI1 (BMI1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. NPY/Neuropeptide Y scFv [L115/13]

    Plasmid

    #190505

    Purpose

    Mammalian Expression of NPY/Neuropeptide Y scFV. Derived from hybridoma L115/13.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    NPY/Neuropeptide Y (Homo sapiens) recombinant scFV (NPY Mouse)
    

    Use

    Tags

    HA, Sortase, 6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lucMAPT-30D

    Plasmid

    #214672

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs downstream of the 5′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lucMAPT-30U

    Plasmid

    #214673

    Purpose

    MAPT Exon 10 reporter containing the MS2 hairpin 30 base pairs upstream of the 3′ splice site

    Depositor

    Eugene Yeo

    Article

    Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

    Insert

    MAPT (MAPT Human)
    

    Use

    Tags

    Firefly Luciferase, Renilla Luciferase, and V5

    Expression

    Mammalian

    Mutation

    Contains Exon 9, Exon 10 with a mutation to intro…

    Promoter

    CMV

    Available since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge