59,541 results

1. HA-NEDD8

   Plasmid

   #18711

   Depositor

   Edward Yeh

   Article

   Kamitani et al J Biol Chem. 1997 Nov 7. 272(45):28557-6

   Insert

   NEDD8 (NEDD8 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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2. hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin

   Plasmid

   #99278

   Purpose

   Dual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistance

   Depositor

   Jordan Miller

   Article

   Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

   Inserts

   H2B-mVenus-IRES-mCherry
   Puro
   

   Use

   Lentiviral

   Tags

   H2B, IRES, PGK, mCherry, and mVenus

   Promoter

   EF1 alpha-human and PGK-mouse

   Available Since

   Nov. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMCL-HA-MAPKK1-R4F [delta(31-51)/S218E/S222D]

   Plasmid

   #40810

   Depositor

   Natalie Ahn

   Article

   Mansour et al Science. 1994 Aug 12;265(5174):966-70.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(31-51), S218E, S222D

   Promoter

   CMV

   Available Since

   Nov. 2, 2012

   Availability

   Academic Institutions and Nonprofits only

4. pMK448 (BromoTag-BSD)

   Plasmid

   #214378

   Purpose

   BromoTag for C-terminal tagging

   Depositor

   Masato Kanemaki

   Article

   Hatoyama et al EMBO Rep. 2024 Sep;25(9):4062-4077. doi:

   Insert

   BromoTag-BSD
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Aug. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-parkin C431S

   Plasmid

   #45877

   Depositor

   Edward Fon

   Article

   Trempe et al Science. 2013 May 9.

   Insert

   Parkin (PRKN Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   C431S mutation (ubiquitin acceptor site)

   Promoter

   CMV

   Available Since

   Nov. 14, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pGIPZ-PD-L1-EGFP

   Plasmid

   #120933

   Purpose

   Express PD-L1-EGFP fusion protein in mammalian cells

   Depositor

   Mien-Chie Hung

   Article

   Yang et al Cell Res. 2018 Aug;28(8):862-864. doi: 1

   Insert

   PD-L1-EGFP
   

   Expression

   Bacterial

   Available Since

   Feb. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. NanoLuc-PM

   Plasmid

   #164784

   Purpose

   Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

   Depositor

   Laszlo Hunyady

   Article

   Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

   Insert

   Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
   

   Tags

   NanoLuc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MigR1-AE9a

   Plasmid

   #12433

   Depositor

   Dong-Er Zhang

   Article

   Yan et al Nat Med. 2006 Aug . 12(8):945-9.

   Insert

   AML1-ETO9a fusion (RUNX1, RUNX1T1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   AML-ETO protein with ETO exon 9a.

   Available Since

   Jan. 4, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. hE-cadherin/Δβ-catenin-pcDNA3

   Plasmid

   #45772

   Depositor

   Barry Gumbiner

   Article

   Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

   Insert

   hE-cadherin/Δβ-catenin (CDH1 Human)
   

   Expression

   Mammalian

   Mutation

   Ends at amino acid 844. Last 35 amino acids dele…

   Promoter

   CMV

   Available Since

   June 14, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. DsRed-rab7 WT

    Plasmid

    #12661

    Depositor

    Richard Pagano

    Article

    Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

    Insert

    RAB7 (Homo sapiens) (RAB7A Human)
    

    Tags

    DsRed and HA

    Expression

    Mammalian

    Available Since

    Oct. 16, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. GFP-PTEN

    Plasmid

    #13039

    Depositor

    Alonzo Ross

    Article

    Liu et al J Cell Biochem. 2005 Oct 1. 96(2):221-34

    Insert

    PTEN (PTEN Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Available Since

    Nov. 3, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMXSIP-FLAG-ATPIF1

    Plasmid

    #204703

    Purpose

    expresses FLAG-ATPIF1 in mammalian cells

    Depositor

    William Kaelin

    Article

    Wyant et al Science. 2022 Aug 5;377(6606):621-629. d

    Insert

    ATP synthase inhibitory factor subunit 1 (ATP5IF1 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Available Since

    Aug. 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PABPC1

    Plasmid

    #155805

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    PABPC1 (PABPC1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. T7_CC_Vpx-SIV_WPRE_IVT_Template

    Plasmid

    #216792

    Purpose

    Template for in vitro transcription of Vpx (SIV).

    Depositor

    Daniel Bauer

    Article

    Levesque et al Nat Biotechnol. 2024 May 28. doi: 10.103

    Insert

    Vpx (SIV)
    

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. (SIM)10

    Plasmid

    #126946

    Purpose

    Bacterial expression plasmid for (SIM)10 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    10 repeats of SUMO interacting motif (SIM) from PIASx each separated by (GGS)4 (PIAS2 Human)
    

    Expression

    Bacterial

    Promoter

    unknown

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PGK1p-Csy4-pA (Construct 2)

    Plasmid

    #55196

    Purpose

    Expresses Csy4 in Mammalian cells for site-specific RNA processing

    Depositor

    Timothy Lu

    Article

    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1

    Insert

    Csy4/Cas6
    

    Use

    Synthetic Biology

    Tags

    6xHIS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 16, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. CL20_mEGFP_NPM1_ALK

    Plasmid

    #205868

    Purpose

    Express mEGFP-tagged fusion protein, NPM1_ALK from patient-derived sequence

    Depositor

    Richard Kriwacki

    Article

    Tripathi et al Nat Commun. 2023 Sep 28;14(1):6008. doi:

    Insert

    NPM1_ALK (NPM1, ALK Human)
    

    Tags

    mEGFP

    Expression

    Mammalian

    Available Since

    Nov. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHR-CD86

    Plasmid

    #223604

    Purpose

    Lentiviral expression of human CD86 in mammalian cells

    Depositor

    Enfu Hui

    Article

    Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

    Insert

    CD86 (CD86 Human)
    

    Use

    Lentiviral; mammalian expression

    Available Since

    Sept. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. WT-MEK1-V5

    Plasmid

    #217297

    Purpose

    Lentivirus vector for expression of MEK1

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. RalGDS-YPet

    Plasmid

    #134929

    Purpose

    Fluorescently tagged affinity reagent

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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