We narrowed to 9,203 results for: sacs

1. CPH3511 (YCp LEU2 Rpb1 P1472A)

   Plasmid

   #91813

   Purpose

   Yeast expression of S. cerevisiae Rpb1 with a P1472A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   RPO21 (RPO21 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   changed proline 1472 to alanine

   Promoter

   Endogenous Rpb1 promoter

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. CPH3567 (pET151_Spt6 1247-1451 R1282H)

   Plasmid

   #91816

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed arginine 1282 to histidine; deleted resid…

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. CPH3495 (pET151_Spt6 1247-1451 K1435A)

   Plasmid

   #91817

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1435 to alanine; deleted residues …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1010-AIDx-CP

   Plasmid

   #135340

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1010 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   CP 1010 nSpyCas9 -hAIDx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. 1058-AIDx-CP

   Plasmid

   #135342

   Purpose

   Expression of circular permutant of nSpyCas9 at amino acid position 1058 encoding truncated human cytosine deaminase, hAIDx, at the C-terminus.

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   CP 1058 nSpyCas9-hAIDx
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIDx-ID-208

   Plasmid

   #135345

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-208
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AIDx-ID-468

   Plasmid

   #135346

   Purpose

   Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   nSpyCas9-hAIDx-ID-468
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   WT

   Promoter

   Available since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pRS314-U6-A34G/A35G/G96C/A97C/U100C/U101C

   Plasmid

   #81239

   Purpose

   U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U101C) for in vivo studies

   Depositor

   Aaron Hoskins

   Article

   Rodgers et al Nucleic Acids Res. 2016 Dec 15;44(22):10

   Insert

   U6 RNA with A34G/A35G/G96C/A97C/U100C/U101C mutation (snR6 Budding Yeast)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   U6 telestem mutant (A34G/A35G/G96C/A97C/U100C/U10…

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPK-352

   Plasmid

   #157922

   Purpose

   pcDNA-CMV-PCYA-IRES-HO1-P2A-FD-P2A-FNR

   Depositor

   Phillip Kyriakakis

   Article

   Kyriakakis et al J Vis Exp. 2021 Jan 12;(167). doi: 10.37

   Inserts

   tPcyA
   IRES
   HO1
   FD
   FNR
   

   Use

   Synthetic Biology; Optogenetics

   Tags

   Mitochondrial Targeting Sequence, Mitochondrial T…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-mCherry-mCherry-FLARE-CKAR

    Plasmid

    #123345

    Purpose

    Red-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    mCherry-mCherry-FLARE-CKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Xpress (TM) tag, …

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3-Cerulean3-Cerulean3-FLARE-AKAR

    Plasmid

    #123335

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-Cerulean3-FLARE-AKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONRG_P4-P1R:TBA2p

    Plasmid

    #128429

    Purpose

    Gateway (Invitrogen) promoter clone (pDONRG_P4-P1R) containing the TBA2 (At1g62060) promoter sequence (1346 base pairs), for use in Three-way Gateway cloning.

    Depositor

    George Haughn

    Article

    McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

    Insert

    TESTA ABUNDANT 2 (AT1G62060 Mustard Weed)
    

    Use

    Gateway promoter entry clone

    Tags

    Expression

    Mutation

    Promoter

    TESTA ABUNDANT 2

    Available since

    Oct. 4, 2019

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pCu-GFP-Atg17(416) (L313D I314K)

    Plasmid

    #121409

    Purpose

    Expressing GFP-Atg17

    Depositor

    Daniel Klionsky

    Article

    Liu et al Curr Biol. 2016 Jan 25;26(2):150-160. do

    Insert

    CUP1p-GFP-ATG17 ORF-CYC1 terminator
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Leucine 313 to Aspartic acid; Isoleucine 314 to …

    Promoter

    CUP1 promoter

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPICZ-pre-Ost1-pro-alphaf-Btl2

    Plasmid

    #117665

    Purpose

    Study secretion efficiency of Btl2

    Depositor

    Benjamin Glick, Pau Ferrer

    Article

    Barrero et al Microb Cell Fact. 2018 Oct 12;17(1):161.

    Insert

    pre-Ost1-pro-alphaf-Btl2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    This plasmid encodes the lipase Btl2 together wit…

    Promoter

    pAOX1

    Available since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEX-6P-1 p37delta2-81

    Plasmid

    #113502

    Purpose

    Bacterial expression of GST-tagged p37 lacking the N-terminal AA 2-81

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p37 (Ubxn2b Mouse)
    

    Use

    Tags

    GST-PreScission protease site

    Expression

    Bacterial

    Mutation

    delta 2-81

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFL His-I3

    Plasmid

    #113513

    Purpose

    Expression of His-I3 in insect cells (Baculovirus system)

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    I3 (PPP1R11 Human)
    

    Use

    Tags

    6*His

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA5-puro-eGFP-HEC1-M4

    Plasmid

    #114036

    Purpose

    Expression of HEC1 M4 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M4 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M4 (K146E, R153E, K156E)

    Promoter

    CMV TetO

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA5-puro-eGFP-HEC1-M2

    Plasmid

    #114035

    Purpose

    Expression of HEC1 M2 mutant tagged with GFP

    Depositor

    Geert Kops

    Article

    Hiruma et al Science. 2015 Jun 12;348(6240):1264-7. d

    Insert

    GFP tagged - HEC1 -M2 (NDC80 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    M2 (K81E, N87E, K89E)

    Promoter

    CMV TetO

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMGF130

    Plasmid

    #96971

    Purpose

    S. pombe lem2 knockout cassette with hygro resistance gene

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    lem2 (lem2 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    whole ORF deletion and replaced with hygro resist…

    Promoter

    N/A

    Available since

    Aug. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMGF169

    Plasmid

    #96996

    Purpose

    ish1 mCherry insertion cassette with G418 resistance gene

    Depositor

    Wesley Sundquist, Adam Frost

    Article

    Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

    Insert

    ish1 (ish1 Fission Yeast)
    

    Use

    Tags

    GST and mCherry

    Expression

    Bacterial

    Mutation

    ish1 C-terminal domain fused with mCherry + G418 …

    Promoter

    N/A

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET151-Vps4(101-437)-Hcp1 K321D

    Plasmid

    #89288

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Asp

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pET151-Vps4(101-437)-Hcp1 R325D

    Plasmid

    #89289

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Asp

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET151-Vps4(101-437)-Hcp1 E322A

    Plasmid

    #87748

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Glu-322 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pET151-Vps4(101-437)-Hcp1 R325A

    Plasmid

    #87749

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Arg-325 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pET151-Vps4(101-437)-Hcp1 K321A

    Plasmid

    #87747

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100, mutated Lys-321 to Ala

    Promoter

    T7

    Available since

    April 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. tet-pLKO shMKP7.v2 puro

    Plasmid

    #87795

    Purpose

    Expresses an inducible short hairpin targeting human MKP7 sequence

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    shMKP7 (DUSP16 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE tight

    Available since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pEN_TTmiRc2 3XFLAG-MKK4-EE-NLS

    Plasmid

    #87776

    Purpose

    Used as a donor vector containing N-term 3xFLAG to clone into pSLIK-Hygro

    Depositor

    Kevin Janes

    Article

    Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

    Insert

    MKK4 (MAP2K4 Human)
    

    Use

    Tags

    3xFLAG and NLS

    Expression

    Mutation

    Promoter

    Available since

    March 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGL3-IRES-miR34a-P6

    Plasmid

    #64790

    Purpose

    Luciferase expression driven by miR-34a promoter P6

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P6 (MIR34A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pGL3-IRES-miR34a-P3

    Plasmid

    #64787

    Purpose

    Luciferase expression driven by miR-34a promoter P3

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P3 (MIR34A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL3-IRES-miR34a-P5

    Plasmid

    #64789

    Purpose

    Luciferase expression driven by miR-34a promoter P5

    Depositor

    Joshua Mendell

    Article

    Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

    Insert

    miR-34a promoter P5 (MIR34A Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge