Addgene

We narrowed to 23,918 results for: nes

Showing: 10161 - 10200 of 23918 results

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pEGFP-H2B-MCS

Plasmid

#184041

Purpose

Cloning of GFP-H2B fusion proteins

Depositor

Lienhard Schmitz

Article

Pfisterer et al Open Biol. 2024 May;14(5):230460. doi: 1

Type

Empty backbone

Use

Tags

GFP and H2B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 10, 2024

Availability

Academic Institutions and Nonprofits only

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HA-MBP-TEVpCS-ADGRE4 CTFdeltaTA-FLAG

Plasmid

#217725

Purpose

Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

Depositor

Stephen Blacklow

Article

Dates et al bioRxiv 2022.07.14.500097

Insert

ADGRE4

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Deletion of residues 1-179

Promoter

Available since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pMMK201

Plasmid

#207131

Purpose

Type 2 ptac promoter part

Depositor

Nicholas Shikuma

Article

Alker et al mBio. 2023 Aug 2:e0150223. doi: 10.1128/

Insert

ptac promoter

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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psiCHECK2 RPS28 UTR1 WT

Plasmid

#203157

Purpose

RPS28 UTR luciferase assay plasmid (WT UTR1, sites 1 and 2)

Depositor

Susan Baserga

Article

Bryant et al Nucleic Acids Res. 2024 Feb 28;52(4):198

Insert

RPS28 UTR (RPS28 Human)

Use

Luciferase

Tags

Expression

Mutation

Promoter

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-PRPF4B_IDG-K

Plasmid

#214463

Purpose

Gateway destination clone of PRPF4B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-PRPF4B (PRPF4B Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-STK19_IDG-K

Plasmid

#214465

Purpose

Gateway destination clone of STK19 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

miniTurbo-V5-STK19 (STK19 Human)

Use

Lentiviral; Gateway destination

Tags

miniTurbo-V5

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pnCas9-AID

Plasmid

#196242

Purpose

Plasmid expressing the nCas9-AID fusion

Depositor

Andrea Puhar

Article

Sharma et al mSystems. 2022 Dec 21:e0104522. doi: 10.

Insert

nickase Cas9 fused to AID

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pWR95

Plasmid

#203916

Purpose

BLINCAR plasmid for gene deletion, no genomic target

Depositor

J. Brian Robertson

Article

Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

Insert

modified MCS

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDA010.188

Plasmid

#206792

Purpose

J23113.dCas9

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Insert

dCas9

Use

Tags

Expression

Mutation

Promoter

J23113

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCK389

Plasmid

#206791

Purpose

Sp.dCas9, pTet.MCP-SoxS, J23119.gRNA

Depositor

James Carothers

Article

Alba Burbano et al Proc Natl Acad Sci U S A. 2023 Jul 25;12

Inserts

dCas9
MCP-SoxS
scRNA

Use

Tags

Expression

Mutation

Promoter

J23119 and Tet

Available since

Sept. 29, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-shHsp90ab1(#c)

Plasmid

#206359

Purpose

AAV plasmid expressing HSP90AB1 shRNA in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

shRNA of Hsp90ab1 (#c)

Use

AAV

Tags

Expression

Mutation

Promoter

human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-RedO-shHsp90ab1 (#a)

Plasmid

#206357

Purpose

AAV plasmid expressing HSP90AB1 shRNA in photoreceptors

Depositor

Connie Cepko

Article

Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

Insert

shRNA #a of Hsp90ab1

Use

AAV

Tags

Expression

Mutation

Promoter

human red opsin

Available since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pMB1-A-ptet-FLAG-dCas9

Plasmid

#190130

Purpose

Level 1 MoClo plasmid containing an aTC inducible FLAG tagged dCas9 for CRISPRi

Depositor

Veronica Godoy-Carter

Article

Brychcy et al ACS Synth Biol. 2023 Aug 16. doi: 10.102

Insert

dCas9

Use

CRISPR

Tags

FLAG

Expression

Mutation

Promoter

Available since

Aug. 24, 2023

Availability

Academic Institutions and Nonprofits only

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NK621

Plasmid

#196405

Purpose

Strong expression of firefly luciferase codon-optimized for S. rosetta

Depositor

Nicole King

Article

Coyle et al Curr Biol. 2023 Aug 2:S0960-9822(23)0092

Insert

Sro5actin-firefly-Sro3actin

Use

Luciferase

Tags

Expression

Mutation

N/A (synthetic)

Promoter

Available since

Aug. 2, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T58-Cterminal-mEmerald

Plasmid

#202353

Purpose

Talin(DeltaR4-10)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-10)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T36-Cterminal-mEmerald

Plasmid

#202357

Purpose

Talin(DeltaR2-12)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR2-12)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T29-Cterminal-mEmerald

Plasmid

#202358

Purpose

Talin(DeltaR1-12)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR1-12)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T48-Cterminal-mEmerald

Plasmid

#202354

Purpose

Talin(DeltaR2-10)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR2-10)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T46-Cterminal-mEmerald

Plasmid

#202355

Purpose

Talin(DeltaR4-12)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-12)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T100-Cterminal-mEmerald

Plasmid

#202352

Purpose

Talin(FullLength)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(FullLength)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T41-Cterminal-mEmerald

Plasmid

#202356

Purpose

Talin(DeltaR1-10)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR1-10)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T36-Nterminal-EGFP

Plasmid

#202369

Purpose

EGFP-Talin(DeltaR2-12)

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

EGFP-Talin(DeltaR2-12)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-XT29-Cterminal-mEmerald

Plasmid

#202364

Purpose

Talin(DeltaR1-12::FlnAIg1-8)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR1-12::FlnAIg1-8)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Talin-T46-Nterminal-EGFP

Plasmid

#202367

Purpose

EGFP-Talin(DeltaR4-12)

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

EGFP-Talin(DeltaR4-12)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-T41-Nterminal-EGFP

Plasmid

#202368

Purpose

EGFP-Talin(DeltaR1-10)

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

EGFP-Talin(DeltaR1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-T29-Nterminal-EGFP

Plasmid

#202370

Purpose

EGFP-Talin(DeltaR1-12)

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

EGFP-Talin(DeltaR1-12)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-XT46-Cterminal-mEmerald

Plasmid

#202361

Purpose

Talin(DeltaR4-12::FlnAIg1-8)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-12::FlnAIg1-8)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-XT41-Cterminal-mEmerald

Plasmid

#202362

Purpose

Talin(DeltaR1-10::FlnAIg1-8)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR1-10::FlnAIg1-8)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-XT58-Cterminal-mEmerald

Plasmid

#202359

Purpose

Talin(DeltaR4-10::FlnAIg1-8)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-10::FlnAIg1-8)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-XT48-Cterminal-mEmerald

Plasmid

#202360

Purpose

Talin(DeltaR2-10::FlnAIg1-8)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR2-10::FlnAIg1-8)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-XT36-Cterminal-mEmerald

Plasmid

#202363

Purpose

Talin(DeltaR2-12::FlnAIg1-8)-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR2-12::FlnAIg1-8)-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 31, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PH-PLCd1-mNGx2

Plasmid

#202752

Purpose

expresses fluorescent protein-tagged lipid biosensor

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

PLCD1v2(1-170): mNG2:mNG2 (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EGFP-PIP5K1BD266K

Plasmid

#202723

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

EGFP:PIP5K1B(D266K) (PIP5K1B Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-Mss4-Kina

Plasmid

#202739

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:S. cerevisiae Mss4 (377-756) (MSS4 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
TagBFP2-Mss4-Kina

Plasmid

#202724

Purpose

expresses fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mTagBFP2:S. cerevisiae Mss4(377-756) (MSS4 Budding Yeast)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pCI-neo-μ3A-(HA)3

Plasmid

#198179

Purpose

Expression of HA-tagged AP-3 μ3A in mammalian cells

Depositor

Juan Bonifacino

Article

Mattera et al PLoS Pathog. 2014 May 15;10(5):e1004107.

Insert

AP-3 μ3A

Use

Tags

triple HA tag

Expression

Mammalian

Mutation

silent substitution in codon 225

Promoter

CMV

Available since

April 18, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNanos1-F40A_X

Plasmid

#147935

Purpose

Mammalian Expression of HsNanos1-F40A

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-F40A (NANOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNanos1-W43E_X

Plasmid

#147936

Purpose

Mammalian Expression of HsNanos1-W43E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-W43E (NANOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNanos1-Y46E_X

Plasmid

#147937

Purpose

Mammalian Expression of HsNanos1-Y46E

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos1-Y46E (NANOS1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-MS2-HA-HsNanos2-del6-22_X

Plasmid

#147939

Purpose

Mammalian Expression of HsNanos2-del6-22

Depositor

Elisa Izaurralde

Article

Bhandari et al Genes Dev. 2014 Apr 15;28(8):888-901. do

Insert

HsNanos2-del6-22 (NANOS2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 10161 - 10200 of 23918 results

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