We narrowed to 7,402 results for: Lif;
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Plasmid#69916PurposeUsed to package AAV9:cTNT.iCre, which specifically transduce cardiomyocytes and express Cre recombinase. Only works in vivo.DepositorInsertimproved cre (iCre) recombinase and tdTomato
UseAAVTagsThe iCre coding frame and tdTomato coding frame i…ExpressionMutationPromoterChicken cardiac troponin TAvailable sinceDec. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
SPLICS NU-MT P2A
Plasmid#210129PurposeDetect the Nucleus-Mitochondria contactDepositorInsertSPLICS NU-MT P2A
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pPBpuro-EGFR(M721)
Plasmid#197379PurposeEncoding human ErbB1 mutant.DepositorInsertEGFR mutant (EGFR Human)
UseTagsExpressionMammalianMutationLysine 721 to MethioninePromoterAvailable sinceAug. 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pAAV-hSyn-LMO7 (mNeonGreen-eKL9h-VChR1)
Plasmid#205097Purposefusion protein of mNeonGreen, eKL9h, and Volvox channelrhodopsin-1 for bioluminescent optogeneticsDepositorInsertmNeonGreen, eKaz luciferase, Volvox Channelrhodopsin 1
UseAAVTagsExpressionMutationPromoterhSynAvailable sinceSept. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
p(HA)HIF1alpha (P402A,P564A)
Plasmid#52636Purposemammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutationDepositorInsertHIF1alpha P402A, P564A (HIF1A Human)
UseTagsHAExpressionMammalianMutationP402A, P564APromoterCMVAvailable sinceApril 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCDNA_MET_D14_3Flag
Plasmid#182495PurposeContains cDNA of the MET gene without the exon 14DepositorInsertMET (MET Human)
UseTagsC-terminal 3xFlag tagExpressionMammalianMutationExon 14 deletedPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Grb10-WT
Plasmid#74501PurposeOverexpression of Grb10 in mammalian cellsDepositorInsertGrb10 (GRB10 Human)
UseTagsFLAGExpressionMammalianMutationL358S, V522A, and V524LPromoterCMVAvailable sinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLVX-Puro-TDP-43-M337V
Plasmid#133759PurposeLentiviral expression of human TDP-43 M337V disease mutant in mammalian cells.DepositorInsertTDP-43 (TARDBP Human)
UseLentiviralTagsExpressionMammalianMutationChanged methionine 337 to valinePromoterCMVAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDual-eGFP(Y203)
Plasmid#63559PurposeExpression of the Mostaza (yellow) spectral variant of eGFP in bacteria and in mammalian cells. Used as a reporter for gene targeting and recombineering in mammalian cells.DepositorInsertHis-T7-eGFP(Y203)
UseLentiviralTagsHis6 and T7ExpressionBacterial and MammalianMutationChanged threonine at position 203 with respect to…PromoterCMV-EF1α hybrid (CEF)Available sinceApril 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE HA Chk2 WT
Plasmid#41901DepositorInsertCHK2 (CHEK2 Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pSPIH6
Plasmid#190676PurposeE. coli plasmid for single step cloning of SUMO-peptide-intein (SPI) fusion proteins (contains SUMO and His-tagged intein)DepositorInsertsSmall ubiquitin-like modifier
Mxe GyrA intein with C-terminal chitin binding domain and His tag
UseTagsChitin binding domain, His tag, and N-terminal Hi…ExpressionBacterialMutationAdded C-terminal his tag and NonePromoterT7Available sinceFeb. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti PGK mKate2-OGT WT (Puro)
Plasmid#154290PurposeLentiviral vector encoding full length mKate2-2xFLAG-OGT (full length human O-GlcNAc transferase, WT)DepositorInsertO-GlcNAc Transferase (OGT Human)
UseLentiviralTags2x FLAG and mKate2ExpressionMammalianMutationWTPromoterPGKAvailable sinceDec. 31, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1.tR4-AcdRS82
Plasmid#174101PurposeExpression of orthogonal Mb tRNA and Acd82 tRNA synthetase for incorporation of acridone in eukaryotic cells.DepositorInsertM. barkeri tRNA synthetase for acridone82
UseTagsExpressionMammalianMutationN311S, C313G, V366A, W382TPromoterCMVAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Delta(Pro-MP)ADAM10-HA
Plasmid#65107PurposeExpression of HA tagged ADAM10 lacking prodomain and metalloprotease domain in mammalian cellsDepositorInsertDelta(Pro-MP)ADAM10 (ADAM10 Human)
UseTagsHAExpressionMammalianMutationdeleted AA 19-455PromoterCMVAvailable sinceNov. 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSAM2_mCherry_Baf60c
Plasmid#72691PurposeDoxycyline regulated expression of Baf60c in mammalian cellsDepositorInsertBrg 1 (SMARCD3 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMV-DoxycyclineAvailable sinceAug. 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
NowGFP /pQE-30
Plasmid#74749PurposeNowGFP gene for bacterial expressionDepositorInsertNowGFP
UseTagsHisTag (with a mutation but still working)ExpressionBacterialMutationWasCFP with substitutions E6K, L42M, T43S, V68M, …PromoterT5Available sinceSept. 16, 2016AvailabilityAcademic Institutions and Nonprofits only