We narrowed to 6,944 results for: SIM;

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pROF141

Plasmid

#155329

Purpose

Binary vector for expression of the PULSE system under the control of PAtUbi10 promoter

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_PAtUbi10-SRDX-NLS-EL222-Tnos_SF_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB

Use

Synthetic Biology

Expression

Plant

Available Since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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gp64-mVEGF-C-HisTag-pENTR

Plasmid

#64065

Purpose

Plasmid encodes mature form of murine VEGF-C preceded by baculoviral gp64 secretion signal; entry vector to Gateway cloning system.

Depositor

Joanna Bereta

Article

Klaus et al Protein Expr Purif. 2015 Mar 7. pii: S10

Insert

vascular endothelial growth factor C (Vegfc Mouse)

Tags

His-tag

Expression

Insect

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST/N1-hEB1-GFP

Plasmid

#27382

Depositor

Robin Shaw

Article

Smyth et al J Clin Invest. 2010 Jan 4. 120(1):266-79

Insert

microtubule-associated protein, RP/EB family, member 1 (MAPRE1 Human)

Tags

GFP

Expression

Mammalian

Available Since

Jan. 27, 2011

Availability

Academic Institutions and Nonprofits only
pLenti-BE3RA-PGK-Puro

Plasmid

#110843

Purpose

Lentiviral vector for constitutive expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

EF1s

Available Since

June 27, 2018

Availability

Academic Institutions and Nonprofits only

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pBRPyCAG-fmKlf4-DsRed-Ip

Plasmid

#26274

Depositor

Rudolf Jaenisch

Article

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Insert

Klf4 (Klf4 Mouse)

Use

Cre/Lox

Expression

Mammalian

Available Since

Sept. 16, 2010

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-F1.GS.CAR-2G

Plasmid

#194461

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.F1 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2z-tal1

Plasmid

#164656

Purpose

for the synthesis of zebrafish tal1 mRNA

Depositor

Didier Stainier

Article

Mattonet et al Sci Adv. 2022 Sep 2;8(35):eabn2082. doi:

Insert

tal1 (tal1 Zebrafish)

Use

Mrna synthesis

Promoter

SP6

Available Since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pQStrep2-GADD45G

Plasmid

#31584

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

growth arrest and DNA-damage-inducible, gamma (GADD45G Human)

Tags

His and Strep

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) pCMV

Plasmid

#8539

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pCW57.1-pDUXChd:hDUX4ctd-ca

Plasmid

#198237

Purpose

Lentivector with tet-inducible porcine DUXC/human DUX4 chimera (codon altered)

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

DUX4L pDUXChd:hDUX4ctd (codon altered) (DUX4 S. scrofa (porcine), Human)

Use

Lentiviral

Expression

Mammalian

Mutation

codon altered

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pETM33_CEP55_EABR

Plasmid

#178469

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

CEP55_EABR (CEP55 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pRRL.SIN.EF1A.JAG1-B5.GS.CAR-2G

Plasmid

#194463

Purpose

CAR featuring: GMCSF (sig. peptide); CD8A & CD8A (hinge & TM); 4-1BB & CD3ζ (signaling domains); anti-JAG1 from J1.B5 in VH-VL order and (GGGGS)3 linker (scFv). GFP-Zeo for selection and monitoring.

Depositor

Ana Sofia Coroadinha

Article

Silva et al Biomolecules. 2023 Mar 2;13(3):459. doi:

Insert

Anti-JAG1 CAR

Use

Lentiviral

Available Since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) FLAG MSCV

Plasmid

#8528

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Missing N-terminal 14 amino acids; improved Ribos…

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
JPF0535a

Plasmid

#124042

Purpose

Encodes pEF1a driving expression of TET3G P2A iRFP713, pEF1a expressing spdCas9 fused to tagBFP P2A tagBFP, and an sgRNA targeting pTRE expressing mAzamiGreen in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-tet3G-P2A-iRFP713_pEF1a-spdCAS9::tagBFP-P2A-tagBFP-SV40PA_mu6-sgTRE_pTRE-NLS::mAzamiGreen-rgPA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-GFP

Plasmid

#82506

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pETM33_EPS15_EH

Plasmid

#178475

Purpose

Bacterial expression of human domain with His-tag and GST tag

Depositor

Ylva Ivarsson

Article

Benz et al Mol Syst Biol. 2022 Jan;18(1):e10584. do

Insert

EPS15_EH (EPS15 Synthetic, Human)

Tags

His-GST

Expression

Bacterial

Promoter

T7/LacO

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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pU6-crRNA(Non-targeting)

Plasmid

#109324

Purpose

AAV vector expressing non-targeting AsCpf1 crRNA

Depositor

Feng Zhang

Article

Tekin et al Nat Biomed Eng. 2018 Jul;2(7):540-554. d

Insert

mCherry-KASH

Use

AAV

Promoter

hSyn1

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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pQTEV-ILKAP

Plasmid

#34817

Depositor

Konrad Buessow

Article

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

integrin-linked kinase-associated serine/threonine phosphatase 2C (ILKAP Human)

Tags

His and TEV

Expression

Bacterial

Available Since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pET41a-MED18

Plasmid

#15433

Depositor

Joan Conaway, Ronald Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED18 (MED18 Human)

Tags

GST and His

Expression

Bacterial

Mutation

N/A

Available Since

Aug. 10, 2007

Availability

Academic Institutions and Nonprofits only