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pLKO.1B POLE1_2

Plasmid

#160765

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_2

Use

Lentiviral

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1B POLE1_1

Plasmid

#160763

Purpose

Suppress POLE1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shPOLE1_1

Use

Lentiviral

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pAc5.1/V5-HisA-RvCAHS12-GS-AcGFP1

Plasmid

#192471

Purpose

Transient expression of RvCAHS12 (fly codon optimized)-GS_linker-AcGFP1 in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS12

Tags

AcGFP1

Expression

Insect

Promoter

Ac5

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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NET97 pmRFP-N2 (641)

Plasmid

#61994

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Korfali et al Mol Cell Proteomics. 2010 Dec;9(12):2571

Insert

AADACL1 (NCEH1 Human)

Tags

mRFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only
human TLNRD1 pET151

Plasmid

#159384

Purpose

Expresses human TLNRD1 in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 (TLNRD1 Human)

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Promoter

T7

Available Since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3_AVPR2-NTEV-TCS-GV-2xHA

Plasmid

#194370

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

AVPR2-NTEV-TCS-GV-2xHA (AVPR2 Human, Synthetic)

Tags

2xHA

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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LentiU6-mNeurog2 gRNA_1-MS2-Puro

Plasmid

#192680

Purpose

Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription

Depositor

Jeffrey Miner

Article

Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

Insert

Mouse Neurog2 activating gRNA #1 (Neurog2 Mouse)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pPKm-245

Plasmid

#90503

Purpose

Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNR

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR

Use

Lentiviral

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pPKm-244

Plasmid

#90502

Purpose

Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A - MTS - tFNR

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR

Use

Lentiviral

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 V2102I

Plasmid

#139322

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 V2102I using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 V2102I using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA2 E2772K

Plasmid

#139323

Purpose

Plasmid expressing a sgRNA to introduce BRCA2 E2772K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA2 E2772K using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEMS1983

Plasmid

#49114

Purpose

AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple253-iCre

Use

AAV

Expression

Mammalian

Promoter

PITX3

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pCK669

Plasmid

#192638

Purpose

Expresses dxCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dxCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Mutation

SoxS has R93A and S101A mutations and dxCas9-NG h…

Promoter

BBa_J23107 and Sp.pCas9

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBabe EGFR Del5(T790M)

Plasmid

#32069

Depositor

Matthew Meyerson

Article

Yuza et al Cancer Biol Ther. 2007 May . 6(5):661-7.

Insert

EGFR (EGFR Human)

Use

Retroviral

Expression

Mammalian

Mutation

Delete E746-S752, insert V, T790M

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
gfp-Arkadia/ RNF111 deltaRING

Plasmid

#112229

Purpose

Expresses GFP-tagged enzymatically inactive (delta RING) Arkadia in mammalian cells

Depositor

Vasso Episkopou

Article

Mavrakis et al PLoS Biol. 2007 Mar;5(3):e67. doi: 10.13

Insert

(Arkadia)Rnf111 (Rnf111 Mouse)

Tags

GFP

Expression

Mammalian

Mutation

delta RING domain deletion (Deletion of the last …

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only
pPKm-112

Plasmid

#90494

Purpose

pcDNA3 - pCMV - MTAD - PIF3, expresses Phytochrome interacting factor 3 (PIF3) and minimal transactivation domain (MTAD), under CMV promoter

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

MTAD-PIF3

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pUC19-A0LWB3

Plasmid

#103070

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

A0LWB3(Cas9 coding gene from Acidothermus cellulolyticus (strain ATCC 43068 / 11B))

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DOCK2-1/1

Plasmid

#91482

Purpose

Protein expression and purification of human SH3 domain construct DOCK2-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DOCK2-1/1 (DOCK2 Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_AMPH-1/1

Plasmid

#91556

Purpose

Protein expression and purification of human SH3 domain construct AMPH-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

AMPH-1/1 (AMPH Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pHH0103_DBNL-1/1

Plasmid

#91409

Purpose

Protein expression and purification of human SH3 domain construct DBNL-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

DBNL-1/1 (DBNL Human)

Expression

Bacterial

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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