Skip to main content
Addgene
Showing: 1001 - 1040 of 84271 results

  11. pGem-sHyper:sAPX

    Plasmid
    #84738
    Purpose
    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Inserts
    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    Use
    Tags
    nuclear localisation signals from the coding sequ…
    Expression
    Plant
    Mutation
    Promoter
    pFMV/p35S
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84738-plasmid-map-sequence-id-180317

    Enlarge

  12. pGem-nHyPer:sAPX

    Plasmid
    #84735
    Purpose
    Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Inserts
    sAPX (SAPX Mustard Weed)
    nHyper (HyPer2 targeted to nucleus)
    Use
    Tags
    nuclear localisation signals from the coding sequ…
    Expression
    Plant
    Mutation
    Promoter
    FMV/35S
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84735-plasmid-map-sequence-id-180316

    Enlarge

  14. pTE3328

    Plasmid
    #107539
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K607R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107539-plasmid-map-sequence-id-207236

    Enlarge

  15. pTE3329

    Plasmid
    #107538
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    G532R, K595R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107538-plasmid-map-sequence-id-207235

    Enlarge

  16. pTE3327

    Plasmid
    #107535
    Purpose
    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    As crRNA
    hAsCpf1(RVR mutant)
    Use
    Tags
    3xHA, NLS, and SV40 NLS
    Expression
    Mammalian
    Mutation
    S542R, K548V, N552R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107535-plasmid-map-sequence-id-207331

    Enlarge

  17. pTE3334

    Plasmid
    #107537
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K582V, N586R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107537-plasmid-map-sequence-id-207346

    Enlarge

  18. pTE3330

    Plasmid
    #107534
    Purpose
    Expresses Lb crRNA and human codon optimized LbCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Lb crRNA
    hLbCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    G532R, K538V, Y542R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107534-plasmid-map-sequence-id-207314

    Enlarge

  19. pTE3336

    Plasmid
    #107541
    Purpose
    Expresses Mb crRNA and human codon optimized MbCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Mb crRNA
    hMbCpf1(RR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N576R, K637R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107541-plasmid-map-sequence-id-207245

    Enlarge

  20. pTE3333

    Plasmid
    #107540
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K671R
    Promoter
    CMV and human U6
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    107540-plasmid-map-sequence-id-207239

    Enlarge

  21. pTE3331

    Plasmid
    #107536
    Purpose
    Expresses Fn crRNA and human codon optimized FnCpf1(RVR mutant) in mammalian cells.
    Depositor
    Ervin Welker
    Article
    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510
    Inserts
    Fn crRNA
    hFnCpf1(RVR mutant)
    Use
    Tags
    3xHA and NLS
    Expression
    Mammalian
    Mutation
    N607R, K613V, N617R
    Promoter
    CMV and human U6
    Available since
    March 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107536-plasmid-map-sequence-id-207338

    Enlarge

  25. pCAG-enAsCas12a(E174R/S542R/K548R)-NLS(nuc)-3xHA (AAS848)

    Plasmid
    #107941
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a (enhanced AsCas12a) encoding E174R/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a (E174R/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R and K548R
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107941-plasmid-map-sequence-id-418295

    Enlarge

  32. pmEos2-N1 GBP (GBP-mEos2)

    Plasmid
    #162876
    Purpose
    Expression in mammalian cells of GFP binding protein (GBP) tagged with photoconvertable protein mEos2 to track GFP proteins of interest to perform Fluorescent intrabody Localization Microscopy
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    GFP Binding Protein tagged with mEos2
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162876-plasmid-map-sequence-id-321989

    Enlarge

  35. pmEos2-N1 PH-PLCd (wild-type) (PH-PLCd-mEos2)

    Plasmid
    #162877
    Purpose
    Expression in mammalian cells of Plekstrin-homology domain of Phospholipase C delta tagged with mEos2 to perform sptPALM
    Depositor
    Frederic Meunier
    Article
    Gormal et al Proc Natl Acad Sci U S A. 2020 Nov 19. p
    Insert
    pleckstrin homology domain of PLCδ1 (phospholipase C-δ1) (PLCD1 Human)
    Use
    Tags
    mEos2
    Expression
    Mammalian
    Mutation
    Insert consists of AA1-170
    Promoter
    Available since
    Jan. 14, 2021
    Availability
    Academic Institutions and Nonprofits only
    162877-plasmid-map-sequence-id-321985

    Enlarge

  36. Anti-MBP [N128A/4R]

    Plasmid
    #206531
    Purpose
    Mammalian Expression Plasmid of anti-Maltose binding protein (MBP) (E. coli). Derived from hybridoma N128A/4.
    Depositor
    James Trimmer
    Article
    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1
    Insert
    anti-Maltose binding protein (MBP) (Escherichia coli) recombinant Mouse monoclonal antibody (malE Mouse)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Dual CMV
    Available since
    Nov. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    206531-plasmid-map-sequence-id-414183

    Enlarge

  37. pcDNA3.3_CoV1_D28

    Plasmid
    #170447
    Purpose
    Express SARS-CoV-1 WT spike protein with a 28aa deletion on the C-terminal tail. Used for SARS1 pseudotyped virus production.
    Depositor
    David Nemazee
    Article
    Rogers et al Science. 2020 Aug 21;369(6506):956-963.
    Insert
    SARS-CoV-1 spike D28 (S SARS-CoV-1)
    Use
    Tags
    NA
    Expression
    Mammalian
    Mutation
    last 28aa deletion in c-terminal tail
    Promoter
    CMV
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    170447-plasmid-map-sequence-id-333040

    Enlarge

  39. pCAG-enAsCas12a-HF1(E174R/N282A/S542R/K548R)-NLS(nuc)-3xHA (AAS1815)

    Plasmid
    #107942
    Purpose
    Mammalian expression plasmid for human codon optimized enAsCas12a-HF1 (enhanced high-fidelity AsCas12a) encoding E174R/N282A/S542R/K548R substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized enAsCas12a-HF1 (E174R/N282A/S542R/K548R)
    Use
    Tags
    3x HA and NLS (nucleoplasmin)
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R, and N282A
    Promoter
    CAG
    Available since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107942-plasmid-map-sequence-id-259760

    Enlarge

Showing: 1001 - 1040 of 84271 results