Skip to main content
Addgene
Showing: 1001 - 1040 of 3893 results

  6. pcDNA3.1_hCAR.Variant3

    Plasmid
    #209133
    Purpose
    The ORF of human constitutive androstane receptor (CAR), variant 3 cDNA amplified from the RT human liver RNA was cloned at the NheI/HindIII sites of the pcDNA3.1(+), the mammalian expression vector.
    Depositor
    Catharine Ross, Reza Zolfaghari
    Insert
    constitutive androstane receptor (CAR), Variant 3 (NR1I3 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    209133-plasmid-map-sequence-id-422366

    Enlarge

  12. pcDXC3GMS

    Plasmid
    #49031
    Purpose
    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, EGFP, Myc tag and streptavidin binding peptide
    Depositor
    Eric Geertsma, Raimund Dutzler
    Article
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Use
    Tags
    3C protease site (PreScission site), EGFP, MYC, a…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    49031-plasmid-map-sequence-id-71989

    Enlarge

  13. pcDXC3MS

    Plasmid
    #49030
    Purpose
    FX cloning mammalian cell line expression vector with CMV promoter and C-terminal 3C protease cleavage site, Myc tag and streptavidin binding peptide
    Depositor
    Eric Geertsma, Raimund Dutzler
    Article
    Geertsma et al Biochemistry. 2011 Apr 19;50(15):3272-8.
    Type
    Empty backbone
    Use
    Tags
    3C protease site (PreScission site), MYC, and str…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 15, 2014
    Availability
    Academic Institutions and Nonprofits only
    49030-plasmid-map-sequence-id-71988

    Enlarge

  15. Ath-HEN1::T2A::mCherry::TY1::H2B-pCFJ350-ttTi5605

    Plasmid
    #108362
    Purpose
    Expression of 2xFlag MYC tagged A.thaliana-HEN1 (Ath-HEN1, codon optimised for C. elegans) and mCherry under the promoter of choice in C. elegans
    Depositor
    Luisa Cochella
    Article
    Alberti et al Nat Methods. 2018 Feb 26. pii: nmeth.461
    Inserts
    Ath-HEN1 (HEN1 Mustard Weed, Codon optimised for C. elegans)
    mCherry
    his-15 (his-15 Nematode)
    unc-54 3' UTR
    Use
    Tags
    2xFlag, MYC, T2A peptide, and TY1 linker
    Expression
    Worm
    Mutation
    Promoter
    A rab-3:Ath-HEN1:unc-54 3' UTR was cloned as…
    Available since
    May 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    108362-plasmid-map-sequence-id-209815

    Enlarge

  25. AA366

    Plasmid
    #215970
    Purpose
    Fragmid fragment: (Pol 2 promoter) Gal4-induced expression
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    5xUAS_v1; 5xUAS_v1; 5xUAS_v1; 5xUAS_v1; Hsp70_v2; Mhc_v1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215970-plasmid-map-sequence-id-431545

    Enlarge

  27. AA365

    Plasmid
    #215969
    Purpose
    Fragmid fragment: (Pol 2 promoter) Gal4-induced expression
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    LoxP_v1; 5xUAS_v1; LoxP_v1; 5xUAS_v1; Hsp70_v1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215969-plasmid-map-sequence-id-431463

    Enlarge

  28. AA371

    Plasmid
    #215989
    Purpose
    Fragmid fragment: (N' terminus) attenuate expression
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    Start; u(S)ORF_v1.1; Stop; Start; HIV-NES_v1.2; HIV-NES_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215989-plasmid-map-sequence-id-431457

    Enlarge

  29. AA401

    Plasmid
    #215959
    Purpose
    Fragmid fragment: (guide cassette) guide expression
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    U6_v1; DR_v1 [Dj]; BsmBI_v1; BsmBI_v12
    Use
    CRISPR; Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215959-plasmid-map-sequence-id-431386

    Enlarge

  30. AA370

    Plasmid
    #215988
    Purpose
    Fragmid fragment: (N' terminus) attenuate expression
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    Start; u(S)ORF_v1.1; Stop; Start; BPNLS_v1.1
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    215988-plasmid-map-sequence-id-431385

    Enlarge

  35. pDONR223_POLR2I_WT_V5

    Plasmid
    #82955
    Purpose
    Gateway Donor vector containing POLR2I, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Jesse Boehm, Matthew Meyerson, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    POLR2I (POLR2I Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82955-plasmid-map-sequence-id-162644

    Enlarge

  40. pDONR223_S100A13_WT_V5

    Plasmid
    #82975
    Purpose
    Gateway Donor vector containing S100A13, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.
    Depositor
    Jesse Boehm, Matthew Meyerson, David Root
    Article
    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do
    Insert
    S100A13 (S100A13 Human)
    Use
    Gateway entry vector
    Tags
    Expression
    Mutation
    Promoter
    None
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    82975-plasmid-map-sequence-id-162776

    Enlarge

Showing: 1001 - 1040 of 3893 results