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Plasmid#25246PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDDB1_1 (DDB1 Human)
UseBaculovirus expressionTagsHisExpressionInsectMutationPromoterpolyhedrinAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGNW2
Plasmid#122086Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAH69
Plasmid#173782PurposeThe pAH69 plasmid [Haldimann and Wanner 2001] is the HK022 helper plasmid necessary for genomic integration of the pIT5_KH plasmid.DepositorInsertHK022 Integrase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-neo
Plasmid#71320PurposeDonor plasmid - kanamycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pmCherry-GFP
Plasmid#86441Purposevisualization and quantifying the out-of-frame mutations from genome editingDepositorInsertmCherry-MCS-sfGFP fusion gene
UseColor visualization in bacteriaTagsmCherry-sfGFPExpressionMutationPromoterPlacAvailable sinceNov. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSEVA6213S
Plasmid#122094Purposeconstitutive expression of I-SceI for genome engineering in PseudomonasDepositorInsertI-SceI restriction endonuclease
UseTagsExpressionMutationPromoterPem7Available sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLacZa-GFP
Plasmid#86440Purposevisualization and quantifying the out-of-frame mutations from genome editingDepositorInsertLacZalpha-MCS-sfGFP fusion gene
UseColor visualization in bacteriaTagsLacZa-sfGFPExpressionMutationPromoterPlacAvailable sinceJan. 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
pSEVA1213S
Plasmid#122095Purposeconstitutive expression of I-SceI for genome engineering in PseudomonasDepositorInsertI-SceI restriction endonuclease
UseTagsExpressionMutationPromoterAvailable sinceMay 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSEVA451-Cpf1
Plasmid#192362PurposeCRISPR-based vector for genome editingDepositorInsertCas12a, gRNA scaffold
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
CYP51A1 (3I3K)
Plasmid#25161PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertCYP51A1_cw_09 (CYP51A1 Human)
UseTagsHisExpressionBacterialMutationContains amino acid residues 54-502PromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
EHMT1 (2IGQ)
Plasmid#25504PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertEHMT1 (EHMT1 Human)
UseTagsHisExpressionBacterialMutationC-terminal domain, residues 951-1235PromoterAvailable sinceAug. 23, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
NRASA
Plasmid#25256PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertNRASA:M1-N172 (NRAS Human)
UseTagsHisExpressionBacterialMutationContains amino acids M1-N172PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGNW4
Plasmid#122088Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGNW6
Plasmid#122093Purposesuicide vector for integration into Pseudomonas genome after insertion of homologous regions at the multiple cloning site; successful integration causes green fluorescence in cellsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLibacceptorV2
Plasmid#106250PurposeRecombination-mediated cassette exchange with target genomic lociDepositorTypeEmpty backboneUseCre/Lox and Synthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEMG-RIS
Plasmid#225132PurposeFor genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 10, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEVA12TEC-I
Plasmid#58308PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS I repA, Epsilon, FRT-kanR-FRT (medium-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12DKR-J
Plasmid#58306PurposeGeneGuard genomic cassette: for constructing ΔdapA::RBS J pi, Kis, FRT-kanR-FRT (high-copy support for R6K COR). Will support pSEVAD1K.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCHAP4
Plasmid#206850PurposeA plasmid containing a 16,121 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-cat
Plasmid#71321PurposeDonor plasmid - chloramphenicol resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTKDP-hph
Plasmid#71322PurposeDonor plasmid - hygromycin resistant donor cassetteDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
EIF4A2
Plasmid#25259PurposeBacterial expression for structure determination; may not be full ORFDepositorInserteIF4A2:G22-E240 (EIF4A2 Human)
UseTagsHisExpressionBacterialMutationContains amino acids G22-E240PromoterAvailable sinceAug. 13, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEVA12DKR-A
Plasmid#58304PurposeGeneGuard genomic cassette: for constructing ΔdapA::RBS A pi, Kis, FRT-kanR-FRT (low-copy support for R6K COR). Will support pSEVAD1K.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceNov. 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
MALT1-DD (2G7R)
Plasmid#25613PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertMALT1-DD (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains Death DomainPromoterAvailable sinceAug. 31, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSEVA12DKR-E
Plasmid#58305PurposeGeneGuard genomic cassette: for constructing ΔdapA::RBS E pi, Kis, FRT-kanR-FRT (medium-copy support for R6K COR). Will support pSEVAD1K.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceDec. 22, 2014AvailabilityAcademic Institutions and Nonprofits only -
GRM1
Plasmid#25353PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertGRM1-C254S:L28-G518 (GRM1 Human)
UseTagsHisExpressionInsectMutationC254S. Contains amino acids L28-G518.PromoterAvailable sinceJuly 29, 2011AvailabilityIndustry, Academic Institutions, and Nonprofits -
ARL5:Q15-L176 (2H17)
Plasmid#25527PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertARL5:Q15-L176 (ARL5A Human)
UseTagsHisExpressionBacterialMutationContains amino acids Q15-L176PromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
SDCCAG10
Plasmid#25610PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSDCCAG10 (CWC27 Human)
UseTagsHisExpressionBacterialMutationCyclophilin_CeCYP16-Like Domain (residues 8-173)PromoterAvailable sinceAug. 30, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
MALT1 (3K0W)
Plasmid#25306PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmalt1.129.326 (MALT1 Human)
UseTagsHisExpressionBacterialMutationamino acid residues 128-326PromoterAvailable sinceAug. 16, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
MALT1 (3BFO)
Plasmid#25216PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertmalt1.226.314 (MALT1 Human)
UseTagsHisExpressionBacterialMutationContains amino acid residues 226-314 of Isoform 2PromoterAvailable sinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYAMTr2GCas
Plasmid#224865PurposeYeast genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceNov. 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYAMTrGCas
Plasmid#224866PurposeYeast genome editingDepositorTypeEmpty backboneUseCRISPRTagsExpressionYeastMutationPromoterAvailable sinceOct. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP8
Plasmid#206857PurposeA plasmid containing a 11,944 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP7
Plasmid#206853PurposeA plasmid containing a 13,748 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceFeb. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP6
Plasmid#206852PurposeA plasmid containing a 15,671 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP5
Plasmid#206851PurposeA plasmid containing a 17,639 bp fragment of the P. tricornutum chloroplast genome. The fragment is flanked by SapI recognition sites, allowing it to be released from the cloning vector pSAP1.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCHAP3_URA
Plasmid#206848PurposeA plasmid containing a 17,271 bp fragment of the P. tricornutum chloroplast genome. The yeast marker URA3 was integrated into the chloroplast fragment, which is flanked by SapI sites.DepositorTypeEmpty backboneUseTagsExpressionBacterial and YeastMutationPromoterAvailable sinceJan. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
NcCas9
Plasmid#194093PurposeExpresses human codon optimized NcCas9. Genome editing in human cells.DepositorInsertNcCas9
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceJan. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSEVA12TEC-B
Plasmid#58307PurposeGeneGuard genomic cassette: for constructing ΔthyA::RBS B repA, Epsilon, FRT-kanR-FRT (low-copy support for ColE2 COR). Will support pSEVAT7Z.DepositorInsertGenomic cassette
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceMarch 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
ARL5:H14-K177 (2H16)
Plasmid#25526PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertARL5:H14-K177 (ARL5A Human)
UseTagsHisExpressionBacterialMutationContains amino acids H14-K177PromoterAvailable sinceAug. 24, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits