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ML1Nmut_in_pEGFP-C1

Plasmid

#67796

Purpose

Mammalian expression vector driving Mouse ML1Nwt (2xML1N domain 7Q mutant version) fused to EGFP

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

ML1N*2(7Q)-EGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-I

Plasmid

#53437

Purpose

xylose-dependent expression of the type-I agrC and agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pGolden-Hyg

Plasmid

#51423

Purpose

Contain one loxP-PGK-Hyg-pA-loxP cassette, used for golden-gate reaction

Depositor

Yonglun Luo

Article

Luo et al Biotechniques. 2014 May 1;56(5):263-8. d

Insert

PGK-Hyg-pA (PGK1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pCellFree_G07

Plasmid

#67141

Purpose

Gateway destination vector for cell-free expression with N-term myc-tag mCherry tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Expression

Mutation

Promoter

Available since

May 18, 2016

Availability

Academic Institutions and Nonprofits only

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pSBL_DK187

Plasmid

#156423

Purpose

Insect cell expression of 3a-sfGFP, needs to be introduced into baculoviral bacmid

Depositor

Stephen Brohawn

Article

Kern et al bioRxiv [Preprint]. 2021 Jan 26:2020.06.

Insert

SARS-CoV-2 3a protein

Use

Tags

sfGFP-His

Expression

Insect

Mutation

Promoter

Polyhedrin

Available since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pT7-agrBD-I

Plasmid

#53439

Purpose

T7-dependent expression of the type-I agrB and agrD genes of S. aureus RN4220

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrBD locus

Use

Synthetic Biology

Tags

V5 epitope, 6x HIS (not expressed on agrB or agrD…

Expression

Bacterial

Mutation

none

Promoter

T7-lac

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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pCellFree_G08

Plasmid

#67142

Purpose

Gateway destination vector for cell-free expression with C-term mCherry myc-tag tag

Depositor

Kirill Alexandrov

Article

Gagoski et al J Biotechnol. 2015 Feb 10;195:1-7. doi:

Type

Empty backbone

Use

Cell-free expression

Tags

His-MYC and mCherry

Expression

Mutation

Promoter

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pMJA179

Plasmid

#128537

Purpose

Expresses anti-Prunus dulcis scFv Ab

Depositor

Marcos Alcocer

Article

de la Cruz et al J Biotechnol. 2016 Jun 10;227:112-9. doi

Insert

scFv Ab clone PD1F6

Use

Tags

Expression

Yeast

Mutation

Promoter

AOX1

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pACYC-Skp-hLYZ

Plasmid

#83924

Purpose

Co-expresses cytoplasmic copies of the Skp protein folding chaperone and wild type human lysozyme..

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Skp
lysozyme (LYZ Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pXylA-agrCA-IV

Plasmid

#53438

Purpose

xylose-dependent expression of the type-IV agrC and type-I agrA genes of S. aureus

Depositor

Cynthia Collins

Article

Marchand et al Biotechnol Bioeng. 2013 Nov;110(11):3003

Insert

agrCA locus

Use

Synthetic Biology

Tags

none

Expression

Bacterial

Mutation

"MVQTS" sequence added to the N-terminu…

Promoter

PxylA

Available since

June 3, 2014

Availability

Academic Institutions and Nonprofits only

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Cav1-miniSOG

Plasmid

#67667

Purpose

Mammalian expression vector driving dog Caveolin1-minSOG

Depositor

Rob Parton

Article

Ariotti et al Dev Cell. 2015 Nov 23;35(4):513-25. doi:

Insert

Dog Cav1-miniSOG

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only

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pAHW/Joseph2-Multitagged Plasmid

Plasmid

#226725

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in Drosophila cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pAHW/Joseph2

Use

Drosophila s2 cell expression

Tags

HA, Myc, GFP, Flag, V5, 6xHis

Expression

Insect

Mutation

Promoter

Act5C

Available since

Jan. 29, 2025

Availability

Academic Institutions and Nonprofits only

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pGEM_ΔndhD2.km

Plasmid

#185532

Purpose

a pGEM-T easy vector containing the kanamycin resistance cassette flanked by the two regions for the double homologous recombination on the ndhD2 locus

Depositor

Paula Tamagnini

Article

Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

Insert

nptII gene flanked by ndhD2 upstream and downstream regions

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGEM_Δflv3.sm

Plasmid

#185533

Purpose

a pGEM-T easy vector containing the streptomycin/spectinomycin resistance cassette flanked by the two regions for the double homologous recombination on the flv3 locus

Depositor

Paula Tamagnini

Article

Spasic et al J Biotechnol. 2022 Dec 10;360:152-159. d

Insert

aadA gene flanked by flv3 upstream and downstream regions

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMH-HA/Joseph2-Multitagged Plasmid

Plasmid

#226726

Purpose

Validates the expression and detection of epitope-tagged proteins of interest in mammalian cells.

Depositor

Richard Cripps

Article

Robinson et al Biotechniques. 2024;76(7):299-309. doi:

Insert

pMH-HA/Joseph2

Use

Tags

HA, Myc, GFP, Flag, V5, His

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pEGFP-C1-BBS8

Plasmid

#218716

Purpose

Expresses N-terminally EGFP-tagged BBS8 in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

Insert

BBS8 (TTC8 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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Ca-LiPPI-CLAPon1.0 (TEVp)

Plasmid

#213409

Purpose

Light-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)

Depositor

Wenjing Wang

Article

Zhou et al J Am Chem Soc. 2022 Dec 21;144(50):22933

Insert

CaM-V5-TEVp

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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Talin-T48-L432G-Cterminal-mEmerald

Plasmid

#202373

Purpose

Talin(DeltaR2-10)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR2-10)-L432G-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-T58-L432G-Cterminal-mEmerald

Plasmid

#202372

Purpose

Talin(DeltaR4-10)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(DeltaR4-10)-L432G-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
Talin-T100-L432G-Cterminal-mEmerald

Plasmid

#202371

Purpose

Talin(FullLength)-L432G-mEmerald

Depositor

Pakorn Kanchanawong

Article

Liu et al Proc Natl Acad Sci U S A. 2015 Sep 1;112

Insert

Talin(FullLength)-L432G-mEmerald

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 1, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge