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pAAV-RAM-d2TTA::TRE-FLEX-MCS-WPRE-pA

Plasmid

#84467

Purpose

Empty pAAV-CRAM plasmid w. multiple cloning site (MCS)

Depositor

Yingxi Lin

Publication

Sorensen et al Elife. 2016 Sep 23;5. pii: e13918. doi:

Type

Empty backbone

Use

AAV

Promoter

pRAM and TRE

Available since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-F-RAM-d2tTA-TRE-mKate2

Plasmid

#140274

Purpose

F-RAM, the Fos-dependent reporter

Depositor

Yingxi Lin

Publication

Sun et al Cell. 2020 Apr 16;181(2):410-423.e17. do

Insert

mKate2

Use

AAV

Promoter

TRE

Available since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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Col1a-frt-hygro-pA

Plasmid

#20730

Depositor

Rudolf Jaenisch

Publication

Beard et al Genesis. 2006 Jan . 44(1):23-8.

Insert

Col1A-frt-hygro-pA

Use

Mouse Targeting

Available since

April 24, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pDG461

Plasmid

#100902

Purpose

SpCas9n (D10A Nickase mutant) with 2A-EGFP and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of DSBs with no off-target cuts.

Depositor

Paul Thomas

Publication

Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

Inserts

humanized CRISPR associated protein 9 Nickase
U6-gRNA scaffold 1
U6-gRNA scaffold 2

Use

CRISPR and Mouse Targeting

Tags

3xFLAG and T2A-EGFP

Expression

Mammalian

Mutation

D10A mutant converts to Nickase

Promoter

CBh and U6

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pCDNA-3xFLAG-Pontin

Plasmid

#51635

Purpose

Mammalian expression of 3xFLAG tagged pontin, N-terminal.

Depositor

Steven Artandi

Publication

Venteicher et al Cell. 2008 Mar 21;132(6):945-57. doi: 10

Insert

Pontin (RUVBL1 Human)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pcDNA3.1-CHRNA7

Plasmid

#62276

Purpose

mammalian expression of human Alpha7 (CHRNA7)

Depositor

Sherry Leonard, Henry Lester

Publication

Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

Insert

CHRNA7 (CHRNA7 Human)

Expression

Mammalian

Promoter

CMV

Available since

May 21, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

11 Articles

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pBRPyCAG-hKlf2-DsRed-Ip

Plasmid

#26277

Depositor

Rudolf Jaenisch

Publication

Hanna et al Proc Natl Acad Sci U S A. 2010 May 18. 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Klf2 (KLF2 Human)

Expression

Mammalian

Available since

Nov. 12, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Su9-EGFP

Plasmid

#23214

Depositor

David Chan

Publication

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Subunit 9 of the F0-ATPase

Tags

EGFP

Expression

Mammalian

Mutation

contains AA 1-69 of subunit 9

Available since

Jan. 28, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pCS-H2B-EGFP

Plasmid

#53744

Purpose

Contains human Histone H2B fused to EGFP in the pCS2+ expression vector

Depositor

Sean Megason

Publication

Megason et al Methods Mol Biol. 2009;546:317-32. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

histone H2B (H2BC21 Human)

Tags

EGFP

Expression

Mammalian

Promoter

simian CMV IE94

Available since

June 27, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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cavin-1-mEGFP

Plasmid

#27709

Depositor

Ari Helenius

Publication

Hayer et al Traffic. 2010 Mar . 11(3):361-82.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

cavin-1 (Cavin1 Mouse)

Tags

monomeric EGFP

Expression

Mammalian

Available since

July 11, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

6 Articles

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pCMVTNT-T7-KPNA7

Plasmid

#26683

Depositor

Bryce Paschal

Publication

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Karyopherin Alpha 7 (KPNA7 Human)

Tags

T7

Expression

Mammalian

Available since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pcDNA3/Myc-DNMT3A2

Plasmid

#36941

Depositor

Arthur Riggs

Publication

Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DNMT3A2 (DNMT3A Human)

Tags

Myc

Expression

Mammalian

Available since

June 22, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pSicoR-mCh-empty

Plasmid

#21907

Purpose

Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

Depositor

Miguel Ramalho-Santos

Publication

Gaspar-Maia et al Nature. 2009 Aug 13;460(7257):863-8. doi

Type

Empty backbone

Use

Lentiviral and RNAi

Expression

Mammalian

Available since

Sept. 30, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles

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pMXs-Dnmt3l

Plasmid

#13356

Depositor

Shinya Yamanaka

Publication

Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

Insert

DNA (cytosine-5-)-methyltransferase 3-like (Dnmt3l Mouse)

Use

Retroviral

Expression

Mammalian

Mutation

None

Available since

Jan. 12, 2007

Availability

Academic Institutions and Nonprofits only
pBabe-puro-IMS-RP

Plasmid

#24535

Depositor

Peter Sorger

Publication

Albeck et al PLoS Biol. 2008 Dec 2. 6(12):2831-52.

Insert

Diablo homolog (DIABLO Human)

Use

Retroviral

Tags

mRFP1

Expression

Mammalian

Mutation

Deleted residues 56-239

Available since

June 11, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP SNAP23

Plasmid

#101914

Purpose

Expresses SNAP23 in mammalian cells

Depositor

Thierry Galli

Publication

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP 23 (SNAP23 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pCS2-MT mouse Axin (Axin MTFu1)

Plasmid

#21287

Depositor

Frank Costantini

Publication

Zeng et al Cell. 1997 Jul 11. 90(1):181-92.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Axin (Axin1 Mouse)

Tags

Myc

Expression

Mammalian

Available since

Dec. 15, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pCAG-TAG

Plasmid

#26771

Depositor

Shankar Srinivas

Publication

Trichas et al BMC Biol. 2008 Sep 15;6:40. doi: 10.1186

Insert

CAG-TdTomato-2A-H2BGFP-SV40pA

Expression

Bacterial

Available since

March 14, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

11 Articles

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pcDNA5FRT-EF-Pdgfralpha-EGFPN

Plasmid

#66787

Purpose

Expression of murine PDGFRalpha tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Publication

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

PDGFRalpha (Pdgfra Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEGFP-MRLC1 T18D, S19D

Plasmid

#35682

Depositor

Tom Egelhoff

Publication

Beach et al BMC Cell Biol. 2011 Dec 2;12:52.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

MRLC1 (MYL9 Human)

Tags

EGFP

Expression

Mammalian

Mutation

T18D and S19D

Promoter

CMV

Available since

April 24, 2012

Availability

Academic Institutions and Nonprofits only

Cited by

12 Articles