We narrowed to 1,093 results for: pcs2

1. pCS2-foxd4l1.1-deltaRII-Cterm

   Plasmid

   #185504

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   Expression

   Mutation

   RII domain and C-term deleted

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCS2-foxd4l1.1-deltaN-myc

   Plasmid

   #185500

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   myc

   Expression

   Mutation

   N-terminus deleted

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-foxd4l1.1-deltaC-myc

   Plasmid

   #185501

   Purpose

   Expression of Xenopus laevis foxd4

   Depositor

   Sally Moody

   Article

   Neilson et al Dev Biol. 2012 May 15;365(2):363-75. doi

   Insert

   foxd4l1.1
   

   Use

   In vitro transcription

   Tags

   myc

   Expression

   Mutation

   C-terminus deleted

   Promoter

   Available Since

   July 12, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCS2+-dCas9-olEzh2(deltaSET)

   Plasmid

   #180217

   Purpose

   Ezh2 (delta SET domain) fused to dCas9 for H3K27me3 epigenome editing control.

   Depositor

   Hiroyuki Takeda

   Article

   Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

   Insert

   enhancer of zeste 2 polycomb repressive complex 2 subunit (deltaSET)
   

   Use

   Tags

   FLAG and dCas9

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCS2 YFP-Smo-Weyer'sAla5

   Plasmid

   #41016

   Depositor

   Rajat Rohatgi

   Article

   Dorn et al Dev Cell. 2012 Sep 12. pii: S1534-5807(1

   Insert

   smoothened homolog (Smo Mouse)
   

   Use

   Tags

   EVC2 ala5 (aa 1177-1220 with amino acids 1185-118…

   Expression

   Mammalian

   Mutation

   YFP inserted after Smo signal sequence. Transfer…

   Promoter

   Available Since

   Nov. 16, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCS2-F1-GGA-F2-GTG-F3-TTG

   Plasmid

   #30701

   Depositor

   Scot Wolfe, Nathan Lawson

   Article

   Zhu et al Development. 2011 Oct;138(20):4555-64.

   Insert

   F1 GGA, F2 GTG, F3 TTG
   

   Use

   Mrna production

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

7. pCS2-F1-GTG-F2-GGT-F3-GAA

   Plasmid

   #30707

   Depositor

   Scot Wolfe, Nathan Lawson

   Article

   Zhu et al Development. 2011 Oct;138(20):4555-64.

   Insert

   F1 GTG, F2 GGT, F3 GAA
   

   Use

   Mrna production

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

8. pCS2-F1-GAG-F2-GAG-F3-AAG

   Plasmid

   #30695

   Depositor

   Scot Wolfe, Nathan Lawson

   Article

   Zhu et al Development. 2011 Oct;138(20):4555-64.

   Insert

   F1 GAG, F2 GAG, F3 AAG
   

   Use

   Mrna production

   Tags

   FLAG

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 9, 2011

   Availability

   Academic Institutions and Nonprofits only

9. XE226 Drosophila Chibby-pCS2P+

   Plasmid

   #16822

   Depositor

   Randall Moon

   Article

   Takemaru et al Nature. 2003 Apr 24. 422(6934):905-9.

   Insert

   Drosophila Chibby (Cby Fly)
   

   Use

   Xenopus expression

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 13, 2008

   Availability

   Academic Institutions and Nonprofits only

10. ZE44 myc-Dapper-2 pCS2P+

    Plasmid

    #17085

    Depositor

    Randall Moon

    Article

    Waxman et al Development. 2004 Dec. 131(23):5909-21.

    Insert

    Dapper-2 (dact2 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    myc

    Expression

    Mutation

    Promoter

    Available Since

    Feb. 1, 2008

    Availability

    Academic Institutions and Nonprofits only

11. p031 pCS2 End-1 ORF

    Plasmid

    #11030

    Depositor

    Ramesh Shivdasani

    Article

    Shoichet et al Proc Natl Acad Sci U S A. 2000 Apr 11. 9

    Insert

    End-1 (end-1 Nematode)
    

    Use

    Xenopus, mammalian, avian, and zebrafish

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Dec. 2, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCS2+_HA-mAID-nanobody (Kless)

    Plasmid

    #117718

    Purpose

    mRNA synthesis of lysine-less HA-mAID-nanobdy for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    codon-optimized and lysine-less mAID for murine e…

    Promoter

    SP6

    Available Since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcs2-ATF4uORF1-2-GFP-IRES-mCherry

    Plasmid

    #226105

    Purpose

    ATF4uORF1-2 stability reporter construct with deletion of SIFI degron helices 1&2 for transient expression in mammalian cells to read out activation of the integrated stress response (ISR)

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    atf4 (ATF4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    contains uORF1-2 of ATF4, whose stability can ser…

    Promoter

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCS2+8-CYP2A6-flag-tev-his

    Plasmid

    #227165

    Purpose

    For overexpression of CYP2A6 in mammalian cells and purification.

    Depositor

    Yimon Aye

    Article

    Liu et al Cell. 2024 Dec 26;187(26):7450-7469.e29.

    Insert

    CYP2A6 (CYP2A6 Human)
    

    Use

    Tags

    Flag and His6

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available Since

    Aug. 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCS2 Notch1 Full Length-6MT

    Plasmid

    #41728

    Depositor

    Raphael Kopan

    Article

    Schroeter et al Nature. 1998 May 28;393(6683):382-6.

    Insert

    Notch-1 Full Length (Notch1 Mouse)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    L1997V; Contains murine Notch-1 protein up to ami…

    Promoter

    Available Since

    Dec. 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-3xFLAG-NLS-SpCas9-NLS

    Plasmid

    #51307

    Purpose

    Cas9 expression plasmid

    Depositor

    Yonglong Chen

    Article

    Guo et al Development. 2014 Jan 8.

    Insert

    3xFLAG-NLS-SpCas9-NLS
    

    Use

    CRISPR; Xenopus tropicalis expression

    Tags

    3x Flag and NLS

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcs2+spCas9 ABE-Umax-nanos

    Plasmid

    #222142

    Purpose

    Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

    Depositor

    Gaurav Varshney

    Article

    Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

    Insert

    Modified TadA-nSpCas9 with nanos UTR
    

    Use

    CRISPR; Zebrafish expression

    Tags

    Bipartite NLS

    Expression

    Mutation

    Promoter

    SP6

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. PCS2-FlipGFP(Casp3 cleavage seq)

    Plasmid

    #124432

    Purpose

    Expresses FlipGFP (caspase-3 cleavage sequence) protease reporter in zebrafish (verified)

    Depositor

    Xiaokun Shu

    Article

    Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

    Insert

    FlipGFP(Casp3 cleavage seq)
    

    Use

    Zebrafish (good expression)

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCS2+ GFP-2xESP3I-IRES-mCherry

    Plasmid

    #230996

    Purpose

    Protein stability reporter construct for transient expression in mammalian cells. Stability of N-terminal GFP-fusion protein can be assessed by flow cytometry by normalizing to mCherry expression.

    Depositor

    Michael Rape

    Article

    Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

    Type

    Empty backbone

    Use

    Tags

    AcGFP1

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcs2-HRI-GFP-IRES-mCherry

    Plasmid

    #226099

    Purpose

    HRI stability reporter construct for transient expression in mammalian cells

    Depositor

    Michael Rape

    Article

    Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

    Insert

    HRI (EIF2AK1 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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