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pET-NCBD + ACTR

Plasmid

#99335

Purpose

Bacterial coexpression of CBP NCBD and NCoA3 binding domains

Depositor

Peter Wright

Article

Demarest et al Nature. 2002 Jan 31;415(6871):549-53.

Inserts

CBP NCBD (2059-2117) (CREBBP Mouse)
SRC3 (1023-1093) (NCOA3 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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HisGB1-Hif1a(776-826) + CBP TAZ1(340-439)

Plasmid

#99343

Purpose

Bacterial expression of mouse CBP TAZ1 domain as coexpression with Hif1a TAD

Depositor

Peter Wright

Article

Sugase et al Protein Expr Purif. 2008 Feb;57(2):108-1

Inserts

Hif1a (776-826) (HIF1A Human)
CBP (340-439)

Use

Tags

His6GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST SNAP29-V5

Plasmid

#69821

Purpose

Expresses SNAP29-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Synaptosomal-associated protein 29 (SNAP29 Human)

Use

Retroviral

Tags

V5

Expression

Mammalian

Mutation

Promoter

cmv

Available since

April 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK1

Plasmid

#138695

Purpose

Expresses a human SIK1-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK1 human (SIK1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Blast-sgSIK2

Plasmid

#138708

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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FCK-ss-Prl-Arch-GFP

Plasmid

#22224

Purpose

Lentiviral expression of Arch-GFP, with N-terminal MHC Class I antigen signal sequence (ss) and Prolactin signal sequence (Prl), for optogenetic inhibition.

Depositor

Edward Boyden

Article

Chow et al Nature. 2010 Jan 7. 463(7277):98-102.

Insert

ss-Prl-Arch-GFP

Use

Lentiviral

Tags

GFP, Prl, and ss

Expression

Mammalian

Mutation

Promoter

CaMKII

Available since

Jan. 6, 2010

Availability

Academic Institutions and Nonprofits only

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pLenti-Hygro-sgSIK3

Plasmid

#138699

Purpose

Expresses a human SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 human (SIK3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST NET1A V5 WT

Plasmid

#69817

Purpose

Expresses NET1A-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

NET1A (NET1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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FUW TetO HA-AMPKa1 AS

Plasmid

#69825

Purpose

Expresses AMPK alpha 1 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

5'-AMP-activated protein kinase catalytic subunit alpha-1 (PRKAA1 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK2

Plasmid

#138696

Purpose

Expresses a human SIK2-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK2 human (SIK2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-SIK3

Plasmid

#138697

Purpose

Expresses a human SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 human (SIK3 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pSECC-sgLKB1-A

Plasmid

#138663

Purpose

Expresses a mouse LKB1-targeting sgRNA, Cas9, and Cre-recombinase

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgLKB1 mouse (Stk11 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZDeltaAL

Plasmid

#99551

Purpose

Bacterial expression of His tagged mouse CBP HAT Brd-ZZ(1079-1751) 1557-1617 replaced by SGGSG

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) DeltaAL (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

replaced 1557-1618 with SGGSG

Promoter

T7

Available since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

Plasmid

#99342

Purpose

Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

Depositor

Peter Wright

Article

Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

Inserts

STAT1 (710-750) (STAT1 Human)
CBP (1764-1855)

Use

Tags

His6GB1

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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HisGB1-CITED2(216-269)+CBPTAZ1(340-439)

Plasmid

#173761

Purpose

Bacterial coexpression of human CITED2 CTAD with CBP TAZ1

Depositor

Peter Wright

Article

Berlow et al Nature. 2017 Mar 16;543(7645):447-451. d

Inserts

CITED2 (216-269) (CITED2 Human)
CBP TAZ1 (340-439) (Crebbp Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti-Hygro-sgTom

Plasmid

#138698

Purpose

Expresses a tomato-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgTomato

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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FUW TetO NET1A-V5 WT

Plasmid

#69828

Purpose

Expresses NET1A-V5 in mammalian cells in a doxycycline inducible manner

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

Neuroepithelial cell-transforming gene 1 protein A (NET1 Human)

Use

Lentiviral

Tags

v5

Expression

Mammalian

Mutation

Promoter

minimal CMV promoter with tetracycline operator

Available since

April 25, 2016

Availability

Academic Institutions and Nonprofits only

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pECE M2-SH3PXD2A 8 serines mutated to alanines

Plasmid

#69816

Purpose

Expresses M2-SH3PXD2A in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

SH3 and PX domain-containing protein 2A (SH3PXD2A Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

SV40 early promoter

Available since

May 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-A

Plasmid

#138682

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-Puro-sgSIK3-C

Plasmid

#138683

Purpose

Expresses a mouse SIK3-targeting sgRNA and Cas9

Depositor

Reuben Shaw

Article

Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

Insert

sgSIK3 mouse (Sik3 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

hU6

Available since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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