We narrowed to 2,372 results for: tub
-
Plasmid#219709PurposeLevel 0 pSPCH-PhiC31-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pSPCH:PhiC31:tUBQ1-GG4
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
L0-T14 GG0-tUBQ1-NLS-NeonGreen-GG1
Plasmid#219697PurposeLevel 0 to construct history-dependent targetDepositorInsertGG0-tUBQ1-NLS-NeonGreen-GG1
UseSynthetic BiologyAvailable SinceJune 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinWT-RNAi-resistant-GFP
Plasmid#205603PurposeTet-inducible expression of WT gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinWT-RNAi-resistant-GFP
TagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240AAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA5/FRT/TO–γ-tubulinΔGTP-RNAi-resistant-GFP
Plasmid#205604PurposeTet-inducible expression of N229A gamma-tubulin in human cells, harbors silent mutations in shRNA target site, FLP-In mediated insertionDepositorInsertγ-tubulinΔGTP-RNAi-resistant-GFP
TagsGFPExpressionMammalianMutationG2228A, C2231T, C2237T, G2240A, N229AAvailable SinceAug. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTol2-F-actin-microtubule plus ends reporters
Plasmid#196134Purposezebrafish expressionDepositorInsertF-actin-microtubule plus ends reporters
UseZebrafish expressionAvailable SinceMarch 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]
Plasmid#149427PurposeThe attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.DepositorInsertsU6.3-gRNAs[TraB, bTub]
Hsp70Bb-Cas9-T2A-eGFP-p10
UseCRISPRTagseGFPExpressionInsectAvailable SinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1-gamma-tubulin(N229A)-TEV-His6
Plasmid#178077PurposeInsect cell expression of gamma-tubulin(N229A)-TEV-His6DepositorInsertgamma-tubulin with an N229A point mutation (TUBG1 Human)
TagsTEV-His6ExpressionInsectMutationChanged Asparagine 229 to AlanineAvailable SinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTub-alpha1-EB3-NeonGreen-T2A-iLifeact-mCherry
Plasmid#175261Purposelabelling of microtubule plus end and F-actin structuresDepositorInsertEB3-NeonGreen-T2A-iLifeact-mCherry (MAPRE3 Human)
TagsmCherry (on iLifeact) and mNeonGreen (on EB3)ExpressionMammalianMutationNone (wt)PromoterpTub-alpha1Available SinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-Tnos
Plasmid#211841PurposePlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantPromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBac[3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP]
Plasmid#86456PurposeLox responder construct with floxed (same orientation; 2xloxP, 2xlox2272) mCherry (cy) body marker and H2Av::EGFP (nu) for Cre dependent cell clone marking; (Transformation plasmid; Black eye marker)DepositorInsert3xP3-gTc'v; Tc'aTub1P-loxP(mCherry)Sv40_Tc'H2Av_EGFP
ExpressionInsectAvailable SinceMarch 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAC-U63-gRNA2.1-tub-miRFP680-T2A-HO1(HA)
Plasmid#250795PurposeDrosophila transgenic vector: nMAGIC vector with tub promoter, 3x HA tags, miRFP680, and HO1. Optimized nMAGIC vector with a far red/infra red FP marker.DepositorTypeEmpty backboneUseCRISPRExpressionInsectAvailable SinceFeb. 18, 2026AvailabilityAcademic Institutions and Nonprofits only -
L0I97 - GG0-pAHP6-PhiC31-NLS-tUBQ1-GG4
Plasmid#219711PurposeLevel 0 pAHP6-PhiC31-NLS-tUBQ1 construct for assembly of two integrase constructs, GG0 and GG4 BsaI sites.DepositorInsertGG0-pAHP6:PhiC31-NLS:tUBQ1-GG4
UseSynthetic BiologyAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211844PurposePlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.DepositorInsertLRR-NEL
UseSynthetic BiologyTags3xNLS-HA and mCherryExpressionPlantMutationC492APromoterUBIQUITIN10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPBJ pFOS-KTR-Gal4-PEST-tub3’ pCMV-TagBFP
Plasmid#176531PurposePiggyBAC vector expressing a FOS promoter driven "KGV" synthetic transcription factor and a CMV promoter driven BFP marker.DepositorInsertsKGV transcription factor
TagBFP
UsePiggybac transposase integration vectorTagsErkKTR, Gal4 DNA binding domain, PEST destabiliza…ExpressionMammalianAvailable SinceMarch 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
874V=βTub-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40
Plasmid#159672PurposePlasmid supports Cas9 expression only in males, Opie2-dsRed tagged, and can be integrated with pBac or phC31.DepositorInsertβTub-NLS-hSpCas9-T2A-GFP
TagsT2A-GFPExpressionInsectAvailable SinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
6xHis-TEV-mCherry-LC3B-Gly (Microtubule-associated proteins 1A/1B light chain 3B)
Plasmid#169168PurposeConjugatable form of LC3B lacking 5 C-terminal amino acids (MKLSV), with C-terminal Glycine120 exposed for lipidation reaction.DepositorInsertMAP1LC3B (MAP1LC3B Human)
Tags6X Histidine Tag, TEV cleavage site, mCherryExpressionBacterialPromoterT7 lac promoterAvailable SinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pBBK70 pHomL-RPL18Bp-Scarlet(rfp)-Link-TUB1-SSA1t-HomR (AmpR)
Plasmid#179054PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Scarlet(rfp)-Link-TUB1, a marker for the microtubulesDepositorInsertRPL18Bp-Scarlet(rfp)-Link-TUB1
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBBK49 pHomL-RPL18Bp-Neon(gfp)-Link-TUB1-SSA1t-HomR (AmpR)
Plasmid#179033PurposeVector containing a yeast integration cassette for RPL18B-driven expression of Neon(gfp)-Link-TUB1, a marker for the microtubulesDepositorInsertRPL18Bp-Neon(gfp)-Link-TUB1
ExpressionYeastAvailable SinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos
Plasmid#211842PurposePlant transient expression cassette for a Dexamethasone-inducible E3-DART ligaseDepositorInsertsLRR-NEL
GAL4-VP16-GR
UseSynthetic BiologyTagsHA, Myc, and mCherryExpressionPlantPromoter6xUAS-35Smini and pUBQ10Available SinceMarch 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-Tnos
Plasmid#211845PurposePlant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.DepositorInsertsLRR-NEL
GAL4-VP16-GR
UseSynthetic BiologyTagsHA, Myc, and mCherryExpressionPlantMutationC492APromoter6xUAS-35Smini and pUBQ10Available SinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only