We narrowed to 9,405 results for: Coli
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Plasmid#179243Purposeprotein purification in E. coliDepositorAvailable SinceMarch 4, 2022AvailabilityAcademic Institutions and Nonprofits only
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pET28A-p53 (1-73) (P12A P13A)
Plasmid#62066PurposeExpression of human p53 (residues 1-73) (P12A, P13A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P12A, P13APromoterT7Available SinceFeb. 23, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P to A)
Plasmid#62069PurposeExpression of human p53 (1-73) (all P to A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; all Prolines changed …PromoterT7Available SinceJan. 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
t1-400
Plasmid#166577PurposeFor expression of human talin head (residues 1-400) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.DepositorInsertTln1 Head (1-400) (TLN1 Human)
UseTagsHis6-tag, Xpress-epitope (DLYDDDDK) and enterokin…ExpressionBacterialMutationresidues 1-400 onlyPromoterAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28A-p53 (1-73) (P27A)
Plasmid#62068PurposeExpression of human p53 (1-73) (P27A) in e. coliDepositorInsertp53 (TP53 Human)
UseTagsHisExpressionBacterialMutationContains TAD residues 1-73; P27APromoterT7Available SinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJExpress401-KDAC4 CD
Plasmid#224251PurposeExpresses human KDAC4 (HDAC4) catalytic domain in E. coliDepositorInsertKDAC4 (HDAC4 Human)
UseTagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 648-1083 (catalytic domain…PromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress401-KDAC7 CD
Plasmid#224341PurposeExpresses human KDAC7 (HDAC7) catalytic domain in E. coliDepositorInsertKDAC7 (HDAC7 Human)
UseTagsTEV-cleavable His6ExpressionBacterialMutationOnly includes residues 521-942 (catalytic domain).PromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJExpress401-KDAC8 H143A
Plasmid#224342PurposeExpresses human KDAC8 (HDAC8) H143A in E. coliDepositorInsertKDAC8 H143A (HDAC8 Human)
UseTagsTEV-cleavable His6ExpressionBacterialMutationHistidine 143 mutated to alaninePromoterT5Available SinceOct. 22, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28:LanIV-Chondromyces
Plasmid#225078PurposeExpresses precursor peptide and lanthipeptide synthetase from Chondromyces LanIV (FAST-RiPPs) in E. coliDepositorInsertLanA (LanIV-Chondromyces)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-56
Plasmid#225073PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-56 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-56)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanI-101
Plasmid#225065PurposeExpresses precursor peptide, lanthipeptide cyclase, and lanthipeptide dehydratase from LanI-101 (FAST-RiPPs) in E. coliDepositorInsertLanA (LanI-101)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2E
Plasmid#225072PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2E (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2E)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET28:LanII-2D
Plasmid#225071PurposeExpresses precursor peptide and lanthipeptide synthetase from LanII-2D (FAST-RiPPs) in E. coliDepositorInsertLanA (LanII-2D)
UseTagsHis6ExpressionBacterialMutationWTPromoterT7Available SinceOct. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔPrD(kf)-SNAP
Plasmid#166150PurposeExpression of N. furzeri DDX5ΔPrD(1-535) mutant in E. coliDepositorInsertDDX5ΔPrD(1-535)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔPrD(1-535)PromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-Smt3-DDX5ΔIDR(kf)-SNAP
Plasmid#166151PurposeExpression of N. furzeri DDX5ΔIDR(1-483) mutant in E. coliDepositorInsertDDX5ΔIDR(1-483)
UseTagsHis10-Smt3 and SNAPExpressionBacterialMutationΔIDR(1-483)PromoterT7Available SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET-hMICU1EF
Plasmid#199180PurposeThis plasmid is used to express a EF-hand disabled human MICU1 protein in E. coli. The protein is fused with a maltose binding protein in the N-terminus, and also contains a C-terminal His6 tag.DepositorInsertMICU1 (MICU1 Human)
UseTagsHis6 and MBP, Thrombin site, TEV siteExpressionMutationD421A, E432K, F433W, F453WPromoterAvailable SinceAug. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSF-G1TMSNKRS
Plasmid#198323PurposetRNA synthetase/tRNA pair for the in vivo incorporation of N6-(((trimethylsilyl)methyl)carbamoyl)-L-lysine (TMSNK), into proteins in E. coli in response to the amber (TAG) codonDepositorInserttRNA synthetase
UseTagsExpressionBacterialMutationPromoterT7Available SinceMay 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETDuet-MerTK_KRmutant-PTPN1
Plasmid#196447PurposeExpression of MerTK Kinase Domain in E. coli and surface entropy reduction mutant for crystallization, coexpress PTPN1DepositorUseTagsExpressionBacterialMutationK591R, K639R, K702R, K865RPromoterT7 promoterAvailable SinceMarch 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pOPINF Ta-sro1 PARP
Plasmid#192549PurposeE. coli expression vector for Triticum aestivum sro1 PARP domain (amino acids 246-434)DepositorInsertSIMILAR TO RCD ONE 1 (SRO1) PARP domain, cultivar Shanrong No. 3 (SR3)
UseTagsHis6, 3C protease cleavage siteExpressionBacterial, Insect, and Mamm…MutationPromoterT7 promoterAvailable SinceDec. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
HisGB1-CITED(216-242)+CBPTAZ1(340-439)
Plasmid#189961PurposeCoexpression of CBP TAZ1 and CITED in E coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6GB1-CITED2(216-269APAA) + CBP TAZ1(340-439)
Plasmid#189963PurposeCoexpression of CBP TAZ1 and CITED2 in E coliDepositorUseTagsExpressionBacterialMutationL243A, E245A, L246APromotert7Available SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)
Plasmid#189962PurposeExpression of CBP TAZ1 and CITED2 in E coliDepositorUseTagsExpressionBacterialMutationPromotert7Available SinceNov. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-HsSyx3
Plasmid#179289PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with N-terminal His6 + GFP + TEV protease cleavage siteDepositorInsertSyx
UseTagsHis6 + GFP + TEV protease cleavage siteExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHsSyx3-MBP
Plasmid#179286PurposeE. coli expression plasmid (T7 promoter) for expression-optimized DNA of human Syx (aa 393-792) with C-terminal TEV protease cleavage site + mature maltose binding protein + His6DepositorInsertSyx
UseTagsTEV protease cleavage site + mature maltose bindi…ExpressionBacterialMutationresidues 393-792 from accession number NP_0010361…PromoterT7Available SinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-SU(Z)12(624-900)-H6
Plasmid#183772PurposeExpress of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.DepositorInsertSU(Z)12 residues 624-900 (Su(z)12 Fly)
UseTagsHis tag at the C-terminusExpressionBacterialMutationPromoterT7Available SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-CBP(1603-2678)
Plasmid#183774PurposeExpress GST-CBP(1603-2678) in E. coli. Purified GST-CBP(1603-2678) was used for in vitro acetylation assays.DepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX-2T-TRX-C751-E3616S
Plasmid#182844Purposemutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG inductionDepositorAvailable SinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSMT3-FBF2 SS/Y
Plasmid#166973PurposeExpresses FBF-2 with SS/Y mutations in repeat 5 in E. coli for purification and crystallization studiesDepositorInsertFBF-2 (fbf-2 Nematode)
UseTagsHis-SUMOExpressionBacterialMutationFBF-2 PUM RNA binding domain; amino acids 164-575…PromoterT7Available SinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pQE30-VRN1-R249E-R289E-R296E
Plasmid#159532PurposeExpresses Arabidopsis thaliana VERNALIZATION1 mutant, with R249E, R289E and R296E mutations, in E. coliDepositorInsertVERNALIZATION1 (VRN1 Mustard Weed)
UseTagsSix-Histidine tagExpressionBacterialMutationThree charge reversal mutations, namely R249E R28…PromoterT5Available SinceDec. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pNB1-LOX
Plasmid#154058PurposeLevel 1, Position 3 Golden Gate vector. ZmUbi-5'UTR:loxP-GUS-nosT-loxP-NAM-B1-nosTDepositorInsertLoxP-flanked GUS and TtNAM-B1
UseSynthetic BiologyTagsExpressionBacterialMutationThe TtNAM-B1 gene sequence was domesticated to re…PromoterZmUbiAvailable SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only