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pTK_BsmBI_LacZ_Citrine_Nanotag_51

Plasmid

#130526

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_32

Plasmid

#130523

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_24

Plasmid

#130522

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_70

Plasmid

#130532

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_75

Plasmid

#130534

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_9

Plasmid

#130516

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_5

Plasmid

#130515

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cherry_Nanotag_109

Plasmid

#130548

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_19_neg_Control

Plasmid

#130561

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_32_neg_Control

Plasmid

#130563

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_66

Plasmid

#130531

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_58

Plasmid

#130527

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Citrine_Nanotag_35

Plasmid

#130524

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_18

Plasmid

#130519

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_17

Plasmid

#130518

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_11

Plasmid

#130517

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac K-Flag M33

Plasmid

#1992

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

M33 (Cbx2 Mouse)

Tags

Flag

Expression

Insect

Mutation

Kozak sequence added before ATG

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pX459V2.0+K848A

Plasmid

#108296

Purpose

pX459 V2.0 (Plasmid #62988) with the K848A mutation

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pFastBac K-M33-Flag

Plasmid

#1993

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

M33 (Cbx2 Mouse)

Tags

Flag

Expression

Insect

Mutation

Kozak sequence added before ATG

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
pcDNA3-PM-deltaCKAR

Plasmid

#222429

Purpose

FRET-based reporter for monitoring deltaPKC activity at the plasma membrane in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Plasma membrane delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Nuc-deltaCKAR

Plasmid

#222430

Purpose

FRET-based reporter for monitoring deltaPKC activity at the nucleus in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Nucleus delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Golgi-deltaCKAR

Plasmid

#222428

Purpose

FRET-based reporter for monitoring deltaPKC activity at the Golgi in cells.

Depositor

Alexandra Newton

Article

Kajimoto et al J Biol Chem. 2010 Dec 31. 285(53):41896-

Insert

Golgi delta-selective C kinase activity reporter

Tags

CFP and YFP

Expression

Mammalian

Available Since

Nov. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA3-2xMARS-PLD

Plasmid

#205237

Purpose

Expresses two repeats of PLEKAH5 aa 143-271 (K163A and R164A) fused to phospholipase D from Streptomyces sp. PMF

Depositor

Jeremy Baskin

Inserts

2xPLEKHA5(143-271)-K163A/R164A-HA-nGFP (PLEKHA5 Human)
phospholipase D (PLD)

Tags

Nuclear Export Sequence and mCherry

Expression

Mammalian

Mutation

amino acids 143-271 of PLEKHA5 (GenBank reference…

Promoter

CMV

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pRAR119

Plasmid

#185051

Purpose

J23108(spel), pHP14 stability hairpin, strong RBS, L34 coding region, trrnB

Depositor

Julius Lucks

Article

Rasmussen et al Nucleic Acids Res. 2022 Dec 9;50(22):127

Insert

L34

Expression

Bacterial

Available Since

July 15, 2024

Availability

Academic Institutions and Nonprofits only

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pTcI39-PRM-wasabi

Plasmid

#195555

Purpose

Temperature-dependent expression of mWasabi reporter with transcription activation by TcI39

Depositor

Mikhail Shapiro

Article

Xiong et al ACS Synth Biol. 2022 Jul 15;11(7):2518-2

Inserts

mWasabi
TcI39

Tags

6x His and AANDENYADAS degradation tag

Expression

Bacterial

Mutation

A67T

Available Since

March 25, 2024

Availability

Academic Institutions and Nonprofits only

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Modular BE4max for GG insertion

Plasmid

#198891

Purpose

Easily insert deaminase of choice into BE4max using Golden Gate cloning

Depositor

Reuben Harris

Article

Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

Insert

CRISPR associated protein 9 (cas9 Streptococcus pyogenes)

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLY171

Plasmid

#184145

Purpose

sgRNA with 3 RNA aptemers BoxB at the 3'-end for CRISPRa

Depositor

Baojun Wang

Article

Liu et al Nat Commun. 2022 Apr 11;13(1):1937. doi:

Insert

sgRNA-LEA2-ex3

Use

Synthetic Biology

Promoter

Plux2

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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SH2-free

Plasmid

#175800

Purpose

Human Nck2 SH2 domain

Depositor

Barry Stoddard

Article

Hallinan et al Commun Biol. 2021 Oct 29;4(1):1240. doi:

Insert

human Nck2 SH2

Tags

6xHis

Expression

Bacterial

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-FLAG-NLS-KRAB-MCS_N-term

Plasmid

#136888

Purpose

Doxycycline-inducible vector backbone containing the KRAB repressor domain with FLAG and NLS sequences, followed by a multiple cloning site (MCS)

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

KRAB repressor domain

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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TetO-FUW-MCS-KRAB_C-term

Plasmid

#136889

Purpose

Doxycycline-inducible vector backbone containing a multiple cloning site (MCS), the KRAB repressor domain and a STOP codon

Depositor

Moritz Mall

Article

Adrian-Segarra et al Methods Mol Biol. 2021;2352:227-236. doi

Insert

KRAB repressor domain

Use

Lentiviral

Tags

None

Expression

Mammalian

Available Since

Aug. 2, 2021

Availability

Academic Institutions and Nonprofits only

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