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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 11,803 results for: KIN

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Showing: 5181 - 5200 of 11803 results

  5. qTAG-N-Puro-mStayGold-LMNB1

    Plasmid
    #227329
    Purpose
    Donor template for Puro-2A-mStayGold insertion into the N-terminus of the LMNB1 locus. For nuclear envelope visualization. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 (Addgene #207770)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Puro-2A-mStayGold (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227329-plasmid-map-sequence-id-451729

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  6. CCR5-DuET

    Plasmid
    #213201
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CCR5 (CCR5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213201-plasmid-map-sequence-id-430764

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  7. qTAG-N-Blast-dTAG-LMNB1

    Plasmid
    #207776
    Purpose
    Donor template for Blast-2A-dTAG insertion into the N-terminus of the LMNB1 locus. To be co-transfected with sgRNA plasmid px330-PITCh-LMNB1 Addgene #207770
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    LMNB1 Homology Arms flanking a Blast-dTAG Cassette (LMNB1 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Available Since
    Nov. 29, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    207776-plasmid-map-sequence-id-416195

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  11. pcDNA3-PKCsub-YPet-NES

    Plasmid
    #138207
    Purpose
    Encodes C-terminal (substrate) fragment of FRET-based bimolecular PKC activity reporter (bimCKAR); cytosol targeted. Use in conjunction with pcDNA3-Cerulean-FHA1-NES
    Depositor
    Jin Zhang
    Article
    Herbst et al J Am Chem Soc. 2011 Apr 20;133(15):5676-
    Insert
    PKCsub-YPet-NES
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    138207-plasmid-map-sequence-id-307410

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  12. pcDNA5-LAP-KNL1-M3

    Plasmid
    #115896
    Purpose
    Used for expression of siRNA-resistant KNL1-M3 (modified codons 258 and 259) from the FRT site of FlpIn cells upon addition of doxycyclin.
    Depositor
    Geert Kops
    Article
    Vleugel et al J Cell Biol. 2013 Dec 23;203(6):943-55.
    Insert
    KNL1-M3 (KNL1 Human)
    Tags
    EYFP
    Expression
    Mammalian
    Mutation
    Fragment of KNL1 containing three KNL1 repeat seq…
    Available Since
    Oct. 12, 2018
    Availability
    Academic Institutions and Nonprofits only
    115896-plasmid-map-sequence-id-224847

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  14. pET-30a(+)-Syk-tSH2

    Plasmid
    #111269
    Purpose
    express murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form, in E.coli strain Rosetta 2 (DE3)
    Depositor
    Carol Post
    Article
    Feng et al Phys Chem Chem Phys. 2016 Feb 17;18(8):5
    Insert
    murine Syk tandem SH2 domains (Ser 8 to Gln 264), the unphosphorylated form (Syk Mouse)
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    July 17, 2018
    Availability
    Academic Institutions and Nonprofits only
    111269-plasmid-map-sequence-id-215599

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  20. pHRIG-PRAS40

    Plasmid
    #53599
    Purpose
    lentiviral expression of constitutively active PRAS40 and EGFP
    Depositor
    Heng Zhao
    Article
    Xiong et al Neurobiol Dis. 2014 Jun;66:43-52. doi: 1
    Insert
    PRAS40 (AKT1S1 Human)
    Use
    Lentiviral
    Tags
    IRES-EGFP and myc
    Expression
    Mammalian
    Mutation
    constitutively active (myristoylated version)
    Promoter
    CMV
    Available Since
    Sept. 8, 2014
    Availability
    Academic Institutions and Nonprofits only
Showing: 5181 - 5200 of 11803 results