We narrowed to 5,913 results for: pcDNA 3.1

1. CoV2-N-P.1

   Plasmid

   #177959

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (P80R, R203K, G204R) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   P80R,R203K,G204R

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. FKBP-CD28TMD-NTEVp(H75E)

   Plasmid

   #137829

   Purpose

   MESA split-TEV protease chain with FKBP Rapamycin ectodomain, CD28 transmembrane domain, N-terminal H75E mutant TEV protease

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FKBP-CD28TMD-NTEVp(H75E)
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Histidine 75 to Glutamic Acid in NTEVp fr…

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pY095

   Plasmid

   #84744

   Purpose

   Expresses huLbCpf1-T2A-GFP and crRNA guide

   Depositor

   Feng Zhang

   Article

   Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

   Inserts

   huLbCpf1
   GFP
   

   Use

   CRISPR

   Tags

   3xHA, NLS, and T2A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. DN-LOXL2

   Plasmid

   #200062

   Purpose

   LOXL2 deletion mutant lacking 1-338 amino acids

   Depositor

   Lakshmi Santhanam

   Article

   Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

   Insert

   human lysyl oxidase like 2 (LOXL2 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   N-terminus amino acids 1-338 truncated.

   Promoter

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. R338G/V339P-LOXL2

   Plasmid

   #200063

   Purpose

   Point mutant of hLOXL2 resistant to processing by Factor Xa

   Depositor

   Lakshmi Santhanam

   Article

   Wang et al Commun Biol. 2023 Apr 7;6(1):375. doi: 1

   Insert

   human lysyl oxidase like 2 (LOXL2 Human)
   

   Use

   Tags

   His

   Expression

   Mammalian

   Mutation

   S300P mutation

   Promoter

   Available since

   April 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA

   Plasmid

   #137830

   Purpose

   MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal L190K mutant TEV protease, M cleavage sequence, and tTA transcription factor

   Depositor

   Joshua Leonard

   Article

   Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

   Insert

   FRB-CD28TMD-CTEVp(L190K)-CS(M)-tTA
   

   Use

   Tags

   3x FLAG

   Expression

   Mammalian

   Mutation

   Mutated Leucine 71 to Lysine in CTEVp fragment

   Promoter

   CMV

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GLG1-2Strep

   Plasmid

   #210362

   Purpose

   Expresses GLG1 fused with 2Strep in mammalian cells

   Depositor

   Michele Pagano

   Article

   Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

   Insert

   GLG1 (GLG1 Human)
   

   Use

   Tags

   2Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Diacylglycerol Kinase Zeta

   Plasmid

   #85454

   Purpose

   expression of Diacylglycerol Kinase Zeta

   Depositor

   Stephen Gee

   Article

   Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

   Insert

   Diacylglycerol Kinase Zeta (DGKZ Human)
   

   Use

   Tags

   3x HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CoV2-N-R203K

   Plasmid

   #177951

   Purpose

   Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (R203K). Used for generating RNA packaging virus-like particles.

   Depositor

   Jennifer Doudna

   Article

   Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

   Insert

   SARS-CoV-2 Nucleocapsid (R203K) (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R203K

   Promoter

   Available since

   Nov. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. HA-EGFP-NEMO-D304N

    Plasmid

    #187898

    Purpose

    Mammalian cell expression of D304N mutation in NEMO

    Depositor

    Erika Holzbaur

    Article

    Harding et al Mol Cell. 2023 Sep 7;83(17):3188-3204.e7

    Insert

    HA-EGFP-NEMO-D304N (Ikbkg Mouse)
    

    Use

    Tags

    EGFP and HA

    Expression

    Mammalian

    Mutation

    D304N

    Promoter

    CMV

    Available since

    April 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Mfn1(K88T)-10xmyc

    Plasmid

    #26050

    Depositor

    David Chan

    Article

    Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

    Insert

    Mfn1 (Mfn1 Mouse)
    

    Use

    Tags

    10xMyc

    Expression

    Mammalian

    Mutation

    K88T GTPase mutant

    Promoter

    Available since

    Aug. 20, 2010

    Availability

    Academic Institutions and Nonprofits only

12. eGFP-SUN1

    Plasmid

    #213515

    Purpose

    Expresses human eGFP-SUN1 in mammalian cells

    Depositor

    Karim Mekhail

    Article

    Shokrollahi et al bioRxiv 2023.05.07.539750

    Insert

    SUN1 (SUN1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    SUN1 has a silent mutation on its gene (nucleotid…

    Promoter

    CMV

    Available since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. ATG9A-2STrep

    Plasmid

    #210359

    Purpose

    Expresses ATG9A fused with 2Strep in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    ATG9A (ATG9A Human)
    

    Use

    Tags

    2Strep

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. NSP3C-Flag

    Plasmid

    #210349

    Purpose

    Expresses the C terminal of SARS-CoV-2 NSP3 fused with Flag in mammalian cells

    Depositor

    Michele Pagano

    Article

    Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

    Insert

    C terminal of SARS-CoV-2 NSP3 (ORF1ab )
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Many synonymous changes due to codon optimization

    Promoter

    Available since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. CoV2-N-G204R

    Plasmid

    #177953

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Nucleocapsid (G204R). Used for generating RNA packaging virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Nucleocapsid (G204R) (N )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    G204R

    Promoter

    Available since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. FLAG HDAC5 S259A/S498A

    Plasmid

    #32216

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser259Ala, Ser498Ala

    Promoter

    CMV

    Available since

    Oct. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

17. mCherry-Climp63(1–192)

    Plasmid

    #186955

    Purpose

    Expresses mCherry-Climp63 with partial luminal domain

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Ckap4 (Ckap4 Mouse)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Only remain partial luminal domain

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBCMV-HaloTag-VPg(FCV)

    Plasmid

    #167311

    Purpose

    Template pDNA to prepare a split CaVT component mRNA

    Depositor

    Hirohide Saito

    Article

    Nakanishi et al Cell Chem Biol. 2021 Jan 20. pii: S2451-

    Insert

    HaloTag-VPg(FCV)
    

    Use

    Synthetic Biology

    Tags

    DYK-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. YFP Rab4DN

    Plasmid

    #99019

    Purpose

    Localization and visualization of the dominant negative form of Rab4

    Depositor

    Narasimhan Gautam

    Article

    O'Neill et al Dev Cell. 2018 Jun 15. pii: S1534-5807(1

    Insert

    Rab4 DN (Rab4 Synthetic, Fly)
    

    Use

    Tags

    YFP

    Expression

    Mammalian

    Mutation

    S22N Dominant negative

    Promoter

    CMV

    Available since

    June 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Dr-EGFR

    Plasmid

    #184004

    Purpose

    Dr-EGFR expression in mammalian cells

    Depositor

    Vladislav Verkhusha

    Article

    Leopold et al J Mol Biol. 2020 Jun 12;432(13):3749-376

    Insert

    Dr-EGFR (EGFR Human)
    

    Use

    Tags

    Deinococcus radiodurans bacterial phytochrome

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge