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pRN3P_membrane_EGFP

Plasmid

#139402

Purpose

in vitro transcription for mRNA

Depositor

Maria-Elena Torres-Padilla

Article

Borsos et al Nature. 2019 May;569(7758):729-733. doi:

Insert

membrane-EGFP

Use

In vitro transcription

Tags

EGFP

Expression

Mutation

Promoter

T3

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBR_attB(bxb)_lox

Plasmid

#183762

Purpose

Bxb1-specific donor plasmid for the cloning of payloads <20 kb

Depositor

Richard Davis

Article

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Type

Empty backbone

Use

Cre/Lox and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-S5E2-GCaMP6f

Plasmid

#135632

Purpose

AAV vector to drive the expression of GCaMP6f in PV cortical interneuronsunder the control of the E2 regulatory element

Depositor

Jordane Dimidschstein

Article

Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.

Has Service

AAV PHP.eB, AAV1, and AAV9

Insert

GCaMP6f

Use

AAV

Tags

Expression

Mutation

None

Promoter

Available since

Aug. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pRK5 FLAG wildtype, catalytic active lipin 1

Plasmid

#32005

Depositor

David Sabatini

Article

Peterson et al Cell. 2011 Aug 5;146(3):408-20.

Insert

lipin1 (Lpin1 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV, SP6

Available since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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OmpA-mNG-sfTq2ox

Plasmid

#124220

Purpose

Periplasmic OM bound mNeongreen superfolder mTurquoise2 ox tandem, ~40 % energy transfer. pNM092 - pTHV037-Ompa-SA1-177-(SA-1)-LEDPPAEL-mNG-sfTq2ox,

Depositor

Tanneke den Blaauwen

Article

Meiresonne et al Mol Microbiol. 2019 Apr;111(4):1025-1038

Insert

OmpA-mNG-sfTq2ox

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3-3XFLAG-AGO2

Plasmid

#136687

Purpose

Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

Depositor

David Bartel

Article

McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

Insert

argonaute RISC Catalytic Component 2 (AGO2 Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5-CEP41_Halo

Plasmid

#135446

Purpose

Mamalian cell expression of HaloTag7 localized at the centrosome

Depositor

Kai Johnsson

Article

Frei et al Nat Commun. 2019 Oct 8;10(1):4580. doi:

Insert

CEP41-HaloTag7

Use

Tags

CEP41

Expression

Mammalian

Mutation

Promoter

CMV/TetO

Available since

Jan. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT wild type

Plasmid

#13639

Depositor

Luca Pellegrini

Article

Sik et al J Biol Chem. 2004 Apr 9. 279(15):15323-9

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pDONR223-MAP4K4

Plasmid

#23486

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K4 (MAP4K4 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only