We narrowed to 6,088 results for: cat.2

1. dCas9-dMSK1

   Plasmid

   #165602

   Purpose

   Expresses Sp dCas9 fused to truncated human MSK1 (42-802)

   Depositor

   Isaac Hilton

   Article

   Li et al Nat Commun. 2021 Feb 9;12(1):896. doi: 1

   Insert

   S. Pyogenes dCas9 with c-terminal truncated human Mitogen- and stress-activated protein kinase-1 (42-802) (RPS6KA5 S. Pyogenes, Synthetic, Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG Tag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES2-eGFP:HA-mTREK-1

   Plasmid

   #224773

   Purpose

   Mammalian expression vector for generating mouse TREK-1

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEGFPN1-gamma-tubulin

   Plasmid

   #87956

   Purpose

   Expresses human gamma-tubulin with a GFP-tag on the C-terminal of the protein

   Depositor

   Maria Alvarado-Kristensson, Ana Carrera

   Article

   Alvarado-Kristensson et al Nat Cell Biol. 2009 Sep;11(9):1081-92. d

   Insert

   gamma-tubulin 1 (TUBG1 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   The protein is a sh-gamma-tubulin resistant gene …

   Promoter

   Available since

   Feb. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET28a-6XHis-SUMO-SIRT2

   Plasmid

   #102622

   Purpose

   Expresses a truncation (aa38-356) of human SIRT2 isoform 1 in bacteria for purification

   Depositor

   Hening Lin

   Article

   Jing et al Cancer Cell. 2016 Mar 14;29(3):297-310.

   Insert

   SIRT2 (SIRT2 Human)
   

   Use

   Tags

   6XHis-SUMO

   Expression

   Bacterial

   Mutation

   Deleted amino acids 1-37 and 357-389

   Promoter

   Available since

   Feb. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pQFlag-USP7 CS puroR

   Plasmid

   #46752

   Purpose

   Retroviral vector that expresses catalytically inactive form of Flag-tagged human USP7

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQHA-USP7 CS puroR

   Plasmid

   #46754

   Purpose

   Retroviral vector that expresses catalytically inactive form of HA-tagged human USP7

   Depositor

   Gordon Peters, Goedele Maertens

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   C223S--catalytically inactive

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG426GAL-EGFP

   Plasmid

   #166143

   Purpose

   EGFP expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG426GAL-mRuby3

   Plasmid

   #166144

   Purpose

   mRuby3 expression under the control of the Galactose-inducible promoter in yeast. Contains 2 um element for high copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   mRuby3
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLentiCRISPRv1_SDHB

   Plasmid

   #177981

   Purpose

   lentiviral vector expressing Cas9 and a sgRNA targeting SDHB

   Depositor

   Jessica Spinelli, David Sabatini

   Article

   Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

   Insert

   sgRNA targeting SDHB (SDHB Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   U6

   Available since

   Dec. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLentiCRISPRv1_SDHA

    Plasmid

    #177980

    Purpose

    lentiviral vector expressing Cas9 and a sgRNA targeting SDHA

    Depositor

    Jessica Spinelli, David Sabatini

    Article

    Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

    Insert

    sgRNA targeting SDHA (SDHA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    U6

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pFastBac HTB DPP9S S730A

    Plasmid

    #169946

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    S730A

    Promoter

    polyhedrin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pFastBac HTB DPP9S D808N

    Plasmid

    #169947

    Purpose

    Purification of catalytically-dead DPP9S from insect cells

    Depositor

    Hao Wu

    Article

    Hollingsworth et al Nature. 2021 Mar 17. pii: 10.1038/s41586

    Insert

    DPP9 (DPP9 Human)
    

    Use

    Tags

    HIS-TEV

    Expression

    Insect

    Mutation

    D808N

    Promoter

    polyhedrin

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPICZ-aC-KHC-BZ

    Plasmid

    #160427

    Purpose

    Expression of c-luciferase from sp. Kornikeria hastingsi carriebowae (Belize)

    Depositor

    Todd Oakley

    Article

    Hensley et al Mol Ecol. 2020 Oct 8. doi: 10.1111/mec.1

    Insert

    luciferase
    

    Use

    Luciferase

    Tags

    6x Histidine tag, Yeast alpha secretion factor, c…

    Expression

    Yeast

    Mutation

    Promoter

    AOX

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. 200 bp Axin2 mutant

    Plasmid

    #25704

    Depositor

    Eric Fearon

    Article

    Leung et al J Biol Chem. 2002 Jun 14. 277(24):21657-

    Insert

    Axin 2 promoter (AXIN2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    TCF DNA binding site at bp -108 to -102 mutated …

    Promoter

    Available since

    June 24, 2010

    Availability

    Academic Institutions and Nonprofits only

15. pCLASP_Crz1(19A)_CLASP_RGS2membrane

    Plasmid

    #133085

    Purpose

    Plasmid contains Crz1* with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-Crz1*-yeLANS). CLASP modulates nuclear localization of Crz1* in response to blue light.

    Depositor

    Hana El-Samad

    Article

    Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

    Insert

    Crz1*-CLASP
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    pADH1

    Available since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGFP AH2 tensin

    Plasmid

    #50514

    Purpose

    This plasmid contains GFP21, which has a sequence similar to YFP, but emission/excitation similar to GFP.

    Depositor

    Kenneth Yamada

    Article

    Pankov et al J Cell Biol. 2000 Mar 6;148(5):1075-90.

    Insert

    Tensin (TNS1 Chicken)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    AA659--761, including actin homology 2 domain of …

    Promoter

    CMV

    Available since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pTol2-DEST-Hsp70-zCreI-mTagBFP2d-2xins (JDW 1002)

    Plasmid

    #229817

    Purpose

    A gateway compatible Tol2 destination vector containing the Hsp70 promoter driving Cre and TagBFP followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

    Plasmid

    #229831

    Purpose

    A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Type

    Empty backbone

    Use

    Tol2 destination vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. PCDNA3.1:mTRESK

    Plasmid

    #224771

    Purpose

    In vitro transcription for Oocyte expression of mTRESK

    Depositor

    Dan Minor

    Article

    Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

    Insert

    Potassium channel subfamily member 18 (Kcnk18 Mouse)
    

    Use

    In vitro transcription for oocyte expression

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Jan. 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5E-EFS (EF1a core) (JDW 1319)

    Plasmid

    #224474

    Purpose

    Gateway compatible 5' entry clone with human alpha 2 globin pause site (to prevent transcription readthrough) and EF1a core promoter. Lacks downstream intron for a lower-activity, compact promoter.

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv 2024.07.13.603267

    Insert

    EFS promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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